THIS TASK ORDER IS FOR THE PROTEOMIC CHARACTERIZATION LABORATORY.

美国国家癌症研究所（NCI）的临床蛋白质组学肿瘤分析联盟（CPTAC）是一项全国性的努力，旨在通过大规模蛋白质组学和基因组分析或蛋白质基因组学的应用来加速对癌症分子基础的理解。NCI支持由Leidos生物医学研究公司（Leidos Biomed）运营的抗体项目，该项目通过位于马里兰州弗雷德里克弗雷德里克国家癌症研究实验室（FNLCR）的Leidos Biomed抗体表征实验室（ACL）全面表征用于癌症研究的亲和试剂。建立了蛋白质组学表征实验室（PCL），用于基于质谱（MS）的全面蛋白质组学表征（全局和磷酸化蛋白质组学）和靶向（适合目的）分析。该任务令（TO）不仅旨在扩大Leidos Biomed PCL分析的检测数量，而且还通过开发额外的翻译后修饰（PTMs）方法来扩大能力，这些方法适合用于iMRM和定制检测开发等应用的目的抗体。Leidos Biomed PCL将为CPTAC/DCTD内的项目开发和实施标准化的蛋白质组学测量（全面和有针对性）。Leidos Biomed PCL配备了两台最先进的质谱仪，为蛋白质和肽分析提供最佳的灵敏度和准确性，并与CPTAC蛋白质组表征中心（PCCs）协调进行蛋白质组学能力基准测试。Leidos Biomed PCL将为NCI研究界提供，以加强转化性癌症研究，优先考虑CPTAC和其他DCTD项目。

The National Cancer Institute (NCI)’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) is a national effort to accelerate the understanding of the molecular basis of cancer through the application of large-scale proteome and genome analysis, or proteogenomics. NCI supports an Antibody Program, operated by Leidos Biomedical Research, Inc (Leidos Biomed), that comprehensively characterizes affinity reagents for cancer research via the Leidos Biomed Antibody Characterization Laboratory (ACL) located at the Frederick National Laboratory for Cancer Research (FNLCR) in Frederick, MD. Within the Leidos Biomed ACL, a Proteome Characterization Laboratory (PCL) was established for comprehensive Mass Spectrometry (MS)-based proteomic characterization (global- and phospho-proteomes) and targeted (fit-for-purpose) assays. This Task Order (TO) aims not only to expand both the number of assays analyzed by the Leidos Biomed PCL, but also broadens the capabilities with method development of additional Post Translational Modifications (PTMs), fit for purpose antibodies for application such as iMRM and custom assay development. The Leidos Biomed PCL will develop and implement standardized proteomic measurements (comprehensive and targeted) for projects within CPTAC/DCTD. The Leidos Biomed PCL is equipped with two state-of-art mass spectrometers to provide the best sensitivity and accuracy for protein and peptide analysis and benchmarked for proteomic competency in coordination with CPTAC Proteome Characterization Centers (PCCs). The Leidos Biomed PCL will be available for the NCI research community to enhance translational cancer research, with priority given to CPTAC and other DCTD programs.