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Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
10494465
负责人：
Terri Lane Foster Bruce
金额：
$ 28.72万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10494465
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Data Analyses Development Education Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Fluorescence-Activated Cell Sorting Fostering Funding Generations Grant Human Resources Image Image Cytometry Imaging Techniques Institution Light Light Cell Location Microscope Microscopy Organelles Phase Positioning Attribute Recording of previous events Research Research Personnel Resolution Resources Sorting - Cell Movement South Carolina Students Targeted Research Technical Expertise Techniques Technology Time Training United States National Institutes of Health Universities Visualization Work base collaborative environment design equipment training experimental study imaging facilities improved innovation interest light microscopy member pathogen success tool university student web page

项目摘要

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.

项目总结/文摘