权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
10494465
负责人：
Terri Lane Foster Bruce
金额：
$ 28.72万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10494465
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Data Analyses Development Education Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Fluorescence-Activated Cell Sorting Fostering Funding Generations Grant Human Resources Image Image Cytometry Imaging Techniques Institution Light Light Cell Location Microscope Microscopy Organelles Phase Positioning Attribute Recording of previous events Research Research Personnel Resolution Resources Sorting - Cell Movement South Carolina Students Targeted Research Technical Expertise Techniques Technology Time Training United States National Institutes of Health Universities Visualization Work base collaborative environment design equipment training experimental study imaging facilities improved innovation interest light microscopy member pathogen success tool university student web page

项目摘要

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.

项目总结/摘要克莱姆森光成像设备（CLIF）将作为成像和细胞分选核心，史诗级眼镜蛇CLIF是位于克莱姆森大学生活的多用户核心设施科学设施。核心是根据博士特里布鲁斯的方向，并设有一套尼康显微镜以及几种高端徕卡显微镜，具有共焦，多光子光谱共焦、STED超分辨率和HyVolution®功能。核心还具有高内容筛选器（GE InCell分析仪2500 HS）和细胞分选设备（BioRad S3 e）。的成像和细胞分选核心的具体目标是：1）促进和促进研究 2）加强技术援助， CLIF的能力;以及3）促进和促进克莱姆森的研究大学和南卡罗来纳州。为实现目标1，将向下列方面提供援助：设计和实施可视化和细胞分选实验的项目负责人，提供先进的技术和设备培训，并提供有组织的讲习班，显微镜和先进的细胞分选。目标2将通过增加第二个完整的- 时间技术员，将协助实验开发，先进的成像和细胞计数。目标3将通过举办关于先进光成像的教育讲习班来实现技术，流式细胞术和分选的原理和用途，以及数据分析。