Clemson Light Imaging Facility (CLIF)

项目概要/摘要 克莱姆森光成像设备（CLIF）将作为成像和细胞分选核心， 史诗级眼镜蛇CLIF是位于克莱姆森大学生活的多用户核心设施 科学设施。核心是根据博士特里布鲁斯的方向，并设有一套尼康 显微镜以及几种高端徕卡显微镜，具有共焦，多光子光谱 共焦、STED超分辨率和HyVolution®功能。核心还具有高 内容筛选器（GE InCell分析仪2500 HS）和细胞分选设备（BioRad S3 e）。的 成像和细胞分选核心的具体目标是：1）促进和促进研究 2）加强技术援助， CLIF的能力;以及3）促进和促进克莱姆森的研究 大学和南卡罗来纳州。为实现目标1，将向下列方面提供援助： 设计和实施可视化和细胞分选实验的项目负责人， 提供先进的技术和设备培训，并提供有组织的讲习班， 显微镜和先进的细胞分选。目标2将通过增加第二个完整的- 时间技术员，将协助实验开发，先进的成像和细胞计数。 目标3将通过举办关于先进光成像的教育讲习班来实现 技术，流式细胞术和分选的原理和用途，以及数据分析。

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.