Clemson Light Imaging Facility (CLIF)

项目摘要/摘要 克莱姆森光成像设备(CLIF)将作为 史诗般的科布雷。CLIF是一个位于克莱姆森大学生活中心的多用户核心设施 科学设施。核心是在特里·布鲁斯博士的指导下，并容纳了一套尼康 显微镜以及几种具有共焦多光子光谱的高端徕卡显微镜 共焦、STED超分辨率和HyVolution®功能。核心也有很高的 内容筛选器(GE Incell Analyzer 2500 HS)和细胞分类设备(BioRad S3e)。这个 成像和细胞分选核心的具体目标是1)促进和促进研究 由科布雷项目负责人承担；2)加强技术援助 CLIF的能力/能力；以及3)促进和促进克莱姆森的研究 大学和南卡罗来纳州。目标1将通过提供援助来实现 设计和实施可视化和细胞分选实验的项目负责人， 提供先进的技术和设备培训，并提供有组织的研讨会 显微镜和先进的细胞分类技术。目标2将通过增加第二个完整- 时间技术员，谁将协助实验发展，先进的成像和细胞学。 目标3将通过举办关于先进光成像的教育研讨会来解决 技术，流式细胞术和分类的原理和用途，以及数据分析。

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.