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Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
10666667
负责人：
Terri Lane Foster Bruce
金额：
$ 29.79万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666667
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Data Analyses Development Education Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Fluorescence-Activated Cell Sorting Fostering Funding Generations Grant Human Resources Image Imaging Techniques Institution Light Light Cell Location Microscope Microscopy Organelles Phase Positioning Attribute Recording of previous events Research Research Personnel Resources Sorting South Carolina Students Targeted Research Technical Expertise Techniques Technology Training United States National Institutes of Health Universities Visualization Work base cell analyzer collaborative environment design equipment training experimental study imaging facilities improved innovation interest light microscopy member multi-photon pathogen skills success superresolution microscopy tool ultra high resolution university student web page

项目摘要

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.

项目概要/摘要克莱姆森光学成像设施 (CLIF) 将作为成像和细胞分选核心史诗般的 COBRE。 CLIF 是位于克莱姆森大学生活中心的多用户核心设施科学设施。核心区由 Terri Bruce 博士负责，并设有一套 Nikon 设备显微镜以及多种具有共焦、多光子光谱的高端徕卡显微镜共焦、STED 超分辨率和 HyVolution® 功能。核心还具有较高的内容筛选仪 (GE InCell Analyzer 2500 HS) 和细胞分选设备 (BioRad S3e)。这成像和细胞分选核心的具体目标是 1) 促进和促进研究由本次 COBRE 的项目负责人承担； 2）加强技术援助 CLIF 的能力； 3）促进和促进克莱姆森大学的研究大学和南卡罗来纳州。目标 1 将通过提供援助来实现设计和实施可视化和细胞分选实验的项目负责人，提供先进技术和设备培训，并组织研讨会显微镜和先进的细胞分选。目标2将通过添加第二个完整的解决方案来解决时间技术员，将协助实验开发、高级成像和细胞计数。目标 3 将通过举办先进光成像教育研讨会来实现技术、流式细胞术和分选的原理和用途以及数据分析。