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Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
10666667
负责人：
Terri Lane Foster Bruce
金额：
$ 29.79万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666667
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Data Analyses Development Education Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Fluorescence-Activated Cell Sorting Fostering Funding Generations Grant Human Resources Image Imaging Techniques Institution Light Light Cell Location Microscope Microscopy Organelles Phase Positioning Attribute Recording of previous events Research Research Personnel Resources Sorting South Carolina Students Targeted Research Technical Expertise Techniques Technology Training United States National Institutes of Health Universities Visualization Work base cell analyzer collaborative environment design equipment training experimental study imaging facilities improved innovation interest light microscopy member multi-photon pathogen skills success superresolution microscopy tool ultra high resolution university student web page

项目摘要

PROJECT SUMMARY/ABSTRACT The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes as well as several high-end Leica microscopes with confocal, multiphoton spectral confocal, STED Super-Resolution, and HyVolution® capabilities. The core also has a high content screener (GE InCell Analyzer 2500 HS) and cell sorting equipment (BioRad S3e). The specific aims of the Imaging and Cell Sorting Core are 1) to promote and facilitate the research undertaken by the project leaders of this COBRE; 2) to enhance the technical assistance capacity/capabilities of the CLIF; and 3) to promote and facilitate research at Clemson University and in the state of South Carolina. Aim 1 will be addressed by providing assistance to project leaders with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on microscopy and advanced cell sorting. Aim 2 will be addressed by the addition of a second full- time technician, who will assist with experiment development, advanced imaging and cytometry. Aim 3 will be addressed by hosting educational workshops on advanced light imaging techniques, the principles and uses of flow cytometry and sorting, and data analysis.

项目摘要/摘要克莱姆森光成像设备(CLIF)将作为史诗般的科布雷。CLIF是一个位于克莱姆森大学生活中心的多用户核心设施科学设施。核心是在特里·布鲁斯博士的指导下，并容纳了一套尼康显微镜以及几种具有共焦多光子光谱的高端徕卡显微镜共焦、STED超分辨率和HyVolution®功能。核心也有很高的内容筛选器(GE Incell Analyzer 2500 HS)和细胞分类设备(BioRad S3e)。这个成像和细胞分选核心的具体目标是1)促进和促进研究由科布雷项目负责人承担；2)加强技术援助 CLIF的能力/能力；以及3)促进和促进克莱姆森的研究大学和南卡罗来纳州。目标1将通过提供援助来实现设计和实施可视化和细胞分选实验的项目负责人，提供先进的技术和设备培训，并提供有组织的研讨会显微镜和先进的细胞分类技术。目标2将通过增加第二个完整- 时间技术员，谁将协助实验发展，先进的成像和细胞学。目标3将通过举办关于先进光成像的教育研讨会来解决技术，流式细胞术和分类的原理和用途，以及数据分析。