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Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
9900818
负责人：
Terri Lane Foster Bruce
金额：
$ 18.13万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2022-07-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9900818
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Development Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Microscopy Fluorescence-Activated Cell Sorting Fostering Funding Generations Goals Grant Guava Fruit Image Imaging Techniques Infrastructure Institution Laboratories Light Light Microscope Location Microscope Microscopy Organelles Preparation Research Research Personnel Research Project Grants Research Technics Resolution Running Sorting - Cell Movement South Carolina Specimen Students Targeted Research Technical Expertise Techniques Technology Time Training Training Support United States National Institutes of Health Universities Visualization Work base collaborative environment confocal imaging design equipment training experimental study fluorescence activated cell sorter device graduate student imaging facilities imaging system improved innovation instrumentation interest light microscopy member multiphoton microscopy pathogen polarized light symposium tool university student user-friendly

项目摘要

Summary: The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the new Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes, a Zeiss LSM 510 confocal microscope, a CytoViva Hyperspectral Imaging System, a Leica DM 750 P polarized light microscope, a Leica DM IL inverted contrasting microscope, a Leica M80 stereomicroscope, a Leica TCS SPE spectral confocal and a Leica SP8X multiphoton spectral confocal microscope. Additionally, the CLIF houses cell counting and sorting equipment, including a Guava EasyCyte HT and a BD Influx FACS cell sorter. The CLIF includes a multi-user specimen preparation laboratory, and a dedicated 20-seat classroom equipped with video conferencing and recording capabilities. The specific aims of the Imaging and Cell Sorting Core are 1) to enhance the cell sorting capabilities of the Clemson Light Imaging Facility (CLIF); 2) to promote and facilitate the research undertaken by the target PIs of this COBRE; and 3) to promote and facilitate research at Clemson University and the state of South Carolina. Aim 1 will be addressed by the addition of a BioRad S3 Cell Sorter (funds from CU Provost) and a dedicated cell sorting technician. Aims 2&3 will be addressed through providing assistance to target investigators with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on multiphoton/confocal imaging and advanced cell sorting.

总结：克莱姆森光成像设备（CLIF）将作为成像和细胞分选核心，史诗眼镜蛇。CLIF是位于新的克莱姆森大学生命科学学院的多用户核心设施设施。核心是在博士特里布鲁斯的指导下，并设有一套尼康显微镜，蔡司 LSM 510共聚焦显微镜，CytoViva高光谱成像系统，Leica DM 750 P偏振光显微镜、Leica DM IL倒置对比显微镜、Leica M80立体显微镜、Leica TCS SPE 光谱共聚焦显微镜和Leica SP 8X多光子光谱共聚焦显微镜。此外，CLIF房屋细胞计数和分选设备，包括Guava EasyCyte HT和BD Influx FACS细胞分选仪。的 CLIF包括一个多用户标本制备实验室和一个专用的20个座位的教室，视频会议和录制功能。成像和细胞分选核心的具体目标是：1）增强细胞分选能力，克莱姆森光成像设施（CLIF）; 2）促进和促进目标PI进行的研究，本COBRE;和3）促进和促进克莱姆森大学和南卡罗来纳州的研究。目标1将通过增加BioRad S3细胞分选仪（由CU Provost提供资金）和专用细胞分选技术员目标2和3将通过向目标调查人员提供援助来实现，设计和实施可视化和细胞分选实验，提供先进的技术和设备培训，并举办多光子/共聚焦成像和先进细胞成像讲习班分类