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Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
9900818
负责人：
Terri Lane Foster Bruce
金额：
$ 18.13万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2022-07-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9900818
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Development Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence Microscopy Fluorescence-Activated Cell Sorting Fostering Funding Generations Goals Grant Guava Fruit Image Imaging Techniques Infrastructure Institution Laboratories Light Light Microscope Location Microscope Microscopy Organelles Preparation Research Research Personnel Research Project Grants Research Technics Resolution Running Sorting - Cell Movement South Carolina Specimen Students Targeted Research Technical Expertise Techniques Technology Time Training Training Support United States National Institutes of Health Universities Visualization Work base collaborative environment confocal imaging design equipment training experimental study fluorescence activated cell sorter device graduate student imaging facilities imaging system improved innovation instrumentation interest light microscopy member multiphoton microscopy pathogen polarized light symposium tool university student user-friendly

项目摘要

Summary: The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the new Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes, a Zeiss LSM 510 confocal microscope, a CytoViva Hyperspectral Imaging System, a Leica DM 750 P polarized light microscope, a Leica DM IL inverted contrasting microscope, a Leica M80 stereomicroscope, a Leica TCS SPE spectral confocal and a Leica SP8X multiphoton spectral confocal microscope. Additionally, the CLIF houses cell counting and sorting equipment, including a Guava EasyCyte HT and a BD Influx FACS cell sorter. The CLIF includes a multi-user specimen preparation laboratory, and a dedicated 20-seat classroom equipped with video conferencing and recording capabilities. The specific aims of the Imaging and Cell Sorting Core are 1) to enhance the cell sorting capabilities of the Clemson Light Imaging Facility (CLIF); 2) to promote and facilitate the research undertaken by the target PIs of this COBRE; and 3) to promote and facilitate research at Clemson University and the state of South Carolina. Aim 1 will be addressed by the addition of a BioRad S3 Cell Sorter (funds from CU Provost) and a dedicated cell sorting technician. Aims 2&3 will be addressed through providing assistance to target investigators with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on multiphoton/confocal imaging and advanced cell sorting.

摘要：克莱姆森光成像设备(CLIF)将作为史诗科布雷。CLIF是一个多用户核心设施，位于新的克莱姆森大学生命科学学院设施。核心是在特里·布鲁斯博士的指导下，并容纳了一套尼康显微镜，一台蔡司 LSM 510共聚焦显微镜，Cytoviva高光谱成像系统，徕卡DM 750P偏振光显微镜，徕卡DM IL倒置对比显微镜，徕卡M80立体显微镜，徕卡TCS SPE 光谱共焦显微镜和徕卡SP8X多光子光谱共焦显微镜。此外，克里夫的房子细胞计数和分选设备，包括一台番石榴EasyCyte HT细胞分选机和一台BD Inflow FACS细胞分选机。这个 CLIF包括一个多用户标本制备实验室和一个配备有视频会议和录制功能。成像和细胞分选核心的具体目标是1)加强克莱姆森光成像设备(CLIF)；2)促进和促进由目标私人投资机构进行的研究 3)促进和促进克莱姆森大学和南卡罗来纳州的研究。目标1将通过增加一个BioRad S3细胞分类器(由CU教务长提供资金)和一个专门的细胞分选技术员。目标2和目标3将通过向目标调查人员提供协助来解决可视化和细胞分选实验的设计和实现，提供先进的技术和设备培训，提供关于多光子/共焦成像和先进细胞的有组织的讲习班分类。