权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanisms of Pathogenesis in Giardiasis

贾第鞭毛虫病的发病机制

基本信息

批准号：
10495245
负责人：
STEVEN M SINGER
金额：
$ 19.5万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-24 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10495245
关键词：
Adjuvant Age Animal Model Biological Assay Body Weight decreased Celiac Disease Cell Culture System Cell Culture Techniques Cell Line Child Chronic Crohn&apos s disease Cysteine Proteinase Inhibitors Data Defect Development Diagnosis Diarrhea Electrical Resistance Enzymes Epithelial Epithelial Cells Fluorescence Microscopy Fractionation Giardia Giardia lamblia Giardiasis Goals Growth Health Herpes zoster disease Human Immune response Immunize Immunoglobulin A In Vitro Income Infection Intervention Intestines Irritable Bowel Syndrome Laboratories Link Malabsorption Syndromes Malnutrition Measurement Monitor Mucous Membrane Mus Myosin Light Chain Kinase Myosin Light Chains Names Nutrient Organoids PAR-2 Receptor Parasites Parasitic infection Pathogenesis Pathology Pathway interactions Peptide Hydrolases Phosphotransferases Pichia Protease Inhibitor Proteinase-Activated Receptors Proteins Protozoan Infections Receptor Signaling Recombinants Recurrence Reporting Role Serine Serine Protease Serine Proteinase Inhibitors Signal Pathway Signal Transduction Structure System Testing Therapeutic Tight Junctions United States Vaccinated Vaccines Virulence Virulence Factors Weight Gain Work antagonist base cognitive development enteric infection fluorescein isothiocyanate dextran immunoregulation in vivo inhibitor insight intestinal barrier intestinal epithelium monolayer novel receptor response

项目摘要

Project Summary Infection with Giardia intestinalis is one of the most common protozoan infections in the intestines of humans worldwide. Recent work has identified chronic and recurrent Giardia infection as a major contributor to stunting in children, and epithelial barrier disfunction is considered a key mechanism linking enteric infections with malnutrition and stunting. Defects in intestinal barrier function have been observed in humans, animal models and epithelial cell cultures infected with Giardia, but the mechanisms responsible for these defects remain poorly described. In the first aim of this proposal we will test the role of specific host signaling pathways in contributing to intestinal carrier disfunction using in vitro and animal models. In the second aim we will determine if parasite proteases are virulence determinants that trigger pathology in vitro and in vivo. Blocking these pathways could greatly reduce the pathology produced during giardiasis.

项目摘要肠贾第鞭毛虫感染是人类肠道中最常见的原虫感染之一全世界。最近的研究发现，慢性和复发性贾第虫感染是导致发育迟缓的主要原因在儿童中，上皮屏障功能障碍被认为是将肠道感染与营养不良和发育迟缓。已在人类和动物模型中观察到肠道屏障功能缺陷以及感染贾第虫的上皮细胞培养物，但导致这些缺陷的机制仍然存在描述得很差。在这项提案的第一个目标中，我们将测试特定的宿主信号通路在使用体外和动物模型导致肠道携带者功能障碍。在第二个目标中，我们将确定寄生虫蛋白酶是否是在体外和体内触发病理的毒力决定因素。阻止这些途径可以极大地减少贾第虫病期间产生的病理。