Development of Optofluidic Resonators for Filoviral Detection

项目摘要/摘要 这份建议书描述了私人助理亚伯拉罕·J·卡维博士、医学博士和博士的四年职业发展计划。 目标是为他的独立研究生涯做准备，成为一名内科科学家。该计划包括扩展 培训机会和通往独立职业的途径，包括发展和实施 传感器技术及其对丝状病毒，即埃博拉病毒的应用。该专业毕业于 来自加州大学欧文分校的生物化学和分子生物学及化学。然后他报名参加了 伊利诺伊大学厄巴纳-香槟分校医学学者计划，在那里他获得了医学博士和博士学位 在化学方面。Qavi博士继续他的医学培训，作为临床病理医生科学家培训的一部分 圣路易斯华盛顿大学和巴恩斯-犹太医院联盟的项目。在他的住院期间 选修时间，他开始在兰阳博士的实验室进行研究，兰阳博士将与他一起担任他的共同导师 阿马拉辛哈医生。杨博士是埃德温·H·斯金纳和弗洛伦斯·G·斯金纳电气与系统系教授 以及国际知名的光子学研究人员。2019年，卡维博士又增加了一项 与病理学和免疫学教授Gaya Amarasinghe博士共同指导下的培训部分， 以扩大他之前开发的传感器技术在传染病领域的应用。 本研究的目标是开发一种快速、多路复用型光流传感器平台，用于 病原体的快速检测，最初的重点是埃博拉。丝状病毒，如埃博拉病毒，属于 大多数致命的人类病原体，在暴发期间病死率很高。关键在于身份识别和 暴发管理是可靠的检测方法，可以快速而灵敏地实施。至 为了满足这一关键需求，将利用密码库模式(WGM)传感器。WGM设备是一类 其中光被限制在微米级体积内的光学传感器。这些设备有令人难以置信的高 该器件具有灵敏度高、传感器占地面积小、易于与传统电子设备集成以及低制造成本等优点。 微泡谐振器(MBR)是WGM传感器的一个子类，具有传统WGM的优点 同时使光学组件和流体组件能够耦合到单个组件中。这样做的目的是 建议使用基于MBR的光流体传感器平台，用于快速、多路检测埃博拉病毒。 工作流程和进展，包括MBR开发、工程抗体、生物物理验证 和多路复用，将提供框架，将工作扩展到最初的目标之外，并促进便利性 卡维博士向独立职业生涯的过渡。

Project Summary/Abstract This proposal describes a 4-year career development plan for the PI, Dr. Abraham J. Qavi, MD, PhD, with the goal of preparing him for an independent research career as a physician scientist. This plan includes expand training opportunities and a pathway to an independent career that includes the development and implementation of sensor technologies and its application towards filoviruses, namely Ebola. The PI graduated with degrees in Biochemistry & Molecular Biology and Chemistry from the University of California, Irvine. He then enrolled in the Medical Scholars Program at the University of Illinois at Urbana-Champaign, where he earned his MD and PhD in Chemistry. Dr. Qavi continued his medical training as part of the Clinical Pathology Physician Scientist Training Program at Washington University in St. Louis and the Barnes-Jewish Hospital consortium. During his residency elective time, he began research in the laboratory of Dr. Lan Yang, who will serve as his co-mentor together with Dr. Amarasinghe. Dr. Yang is the Edwin H. and Florence G. Skinner Professor of Electrical & Systems Engineering, and an internationally renowned researcher in photonics. In 2019, Dr. Qavi added an additional training component under the co-mentorship with Dr. Gaya Amarasinghe, Professor of Pathology & Immunology, to expand the application of his previously developed sensor technology to infectious disease reach. The goal of this study is the development of a rapid, multiplexed optofluidic sensor platform for the rapid detection of pathogens, with an initial focus on Ebola. Filoviruses, such as Ebola, are among the most lethal human pathogens, with high case fatality rates during outbreaks. Critical in the identification and management of outbreaks are robust detection methods that can be implemented rapidly and sensitively. To address this critical need, Whispering Gallery Mode (WGM) sensors will be leveraged. WGM devices are a class of optical sensors in which light is confined within a micron-scale volume. These devices have incredibly high sensitivity, small sensor footprint, ease of integration with conventional electronics, and low fabrication costs. Microbubble resonators (MBRs), a subclass of WGM sensors, offer the advantages of conventional WGM devices while enabling coupling of optical and the fluidic components into a single component. The goal of this proposal is the use of an optofluidic sensor platform based on MBRs for the rapid, multiplexed detection of Ebola. The workflow and advancements, including MBR development, engineered antibodies, biophysical validation and multiplexing, will provide the framework to extend the work beyond the initial goals and promote facile transition to an independent career for Dr. Qavi.