Development of Optofluidic Resonators for Filoviral Detection

用于丝状病毒检测的光流控谐振器的开发

• 批准号: 10668494
• 负责人: Abraham Jaleel Qavi
• 金额: $ 18.97万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668494
• 关键词: 2019-nCoV; Address; Affinity; Africa; Africa South of the Sahara; Area; Binding; Biochemistry; Biological Assay; Biophysics; Biosensing Techniques; Buffers; COVID-19 pandemic; California; Case Fatality Rates; Cessation of life; Chemistry; Clinical Pathology; Cold Chains; Communicable Diseases; Complex; Coupling; Data; Democratic Republic of the Congo; Detection; Development; Development Plans; Devices; Diagnostic; Disease; Disease Outbreaks; Doctor of Philosophy; Ebola; Ebola Hemorrhagic Fever; Ebola virus; Electronics; Engineering; Enrollment; Enzyme-Linked Immunosorbent Assay; Filovirus; Foundations; Glycoproteins; Goals; Hospitals; Illinois; Immune response; Immunology; Individual; Infection; International; Laboratories; Lasers; Lassa virus; Lead; Light; Liquid substance; Measurement; Medical; Mentors; Mentorship; Methods; Microbubbles; Molecular Biology; Monoclonal Antibodies; Optics; Output; Pathogen detection; Pathogenicity; Pathology; Pathway interactions; Performance; Phase; Physicians; Positioning Attribute; Process; Protein Biochemistry; Protein Engineering; RNA Viruses; Reagent; Reporter; Research; Research Personnel; Residencies; Sampling; Scholars Program; Scientist; Sentinel; Serology; Signal Transduction; Source; System; Techniques; Testing; Time; Training; Universities; Validation; Vesicular stomatitis Indiana virus; Viral; Viral Genome; Viral Hemorrhagic Fevers; Viral Markers; Viral Proteins; Virus; Virus Diseases; Washington; Work; analytical tool; antibody engineering; antibody mimetics; career; career development; design; detection method; diagnostic assay; emerging pathogen; follower of religion Jewish; human pathogen; manufacturing cost; multiplex detection; nanoparticle; new outbreak; novel; novel diagnostics; optical sensor; particle; pathogen; photonics; physician-scientist training program; professor; programs; rapid detection; response; sensor; sensor technology; standard of care; training opportunity; viral detection; virology

Project Summary/Abstract This proposal describes a 4-year career development plan for the PI, Dr. Abraham J. Qavi, MD, PhD, with the goal of preparing him for an independent research career as a physician scientist. This plan includes expand training opportunities and a pathway to an independent career that includes the development and implementation of sensor technologies and its application towards filoviruses, namely Ebola. The PI graduated with degrees in Biochemistry & Molecular Biology and Chemistry from the University of California, Irvine. He then enrolled in the Medical Scholars Program at the University of Illinois at Urbana-Champaign, where he earned his MD and PhD in Chemistry. Dr. Qavi continued his medical training as part of the Clinical Pathology Physician Scientist Training Program at Washington University in St. Louis and the Barnes-Jewish Hospital consortium. During his residency elective time, he began research in the laboratory of Dr. Lan Yang, who will serve as his co-mentor together with Dr. Amarasinghe. Dr. Yang is the Edwin H. and Florence G. Skinner Professor of Electrical & Systems Engineering, and an internationally renowned researcher in photonics. In 2019, Dr. Qavi added an additional training component under the co-mentorship with Dr. Gaya Amarasinghe, Professor of Pathology & Immunology, to expand the application of his previously developed sensor technology to infectious disease reach. The goal of this study is the development of a rapid, multiplexed optofluidic sensor platform for the rapid detection of pathogens, with an initial focus on Ebola. Filoviruses, such as Ebola, are among the most lethal human pathogens, with high case fatality rates during outbreaks. Critical in the identification and management of outbreaks are robust detection methods that can be implemented rapidly and sensitively. To address this critical need, Whispering Gallery Mode (WGM) sensors will be leveraged. WGM devices are a class of optical sensors in which light is confined within a micron-scale volume. These devices have incredibly high sensitivity, small sensor footprint, ease of integration with conventional electronics, and low fabrication costs. Microbubble resonators (MBRs), a subclass of WGM sensors, offer the advantages of conventional WGM devices while enabling coupling of optical and the fluidic components into a single component. The goal of this proposal is the use of an optofluidic sensor platform based on MBRs for the rapid, multiplexed detection of Ebola. The workflow and advancements, including MBR development, engineered antibodies, biophysical validation and multiplexing, will provide the framework to extend the work beyond the initial goals and promote facile transition to an independent career for Dr. Qavi.

项目总结/摘要 本提案描述了主要研究者Abraham J. Qavi博士（医学博士、博士）的4年职业发展计划， 他的目标是为他作为一名医生科学家的独立研究生涯做好准备。该计划包括扩大 培训机会和通往独立职业生涯的途径，包括开发和实施 传感器技术及其对丝状病毒，即埃博拉病毒的应用。PI毕业于 生物化学与分子生物学和化学，来自加州大学欧文分校。然后他加入了 伊利诺伊大学厄巴纳-香槟分校的医学学者项目，在那里他获得了医学博士和博士学位 在化学。Qavi博士继续他的医学培训，作为临床病理学医师科学家培训的一部分 圣路易斯华盛顿大学和巴恩斯犹太医院财团的项目。在他住院期间 选修时间，他开始在杨澜博士的实验室进行研究，杨澜博士将担任他的共同导师， 博士阿马拉辛哈杨博士是埃德温·H。和佛罗伦萨G.电气与系统的斯金纳教授 他是国际知名的光子学研究者。2019年，Qavi博士又增加了一个 与病理学和免疫学教授Gaya Amarasinghe博士共同指导的培训部分， 将他先前开发的传感器技术应用到传染病领域。 本研究的目标是开发一种快速、多路复用的光流体传感器平台， 快速检测病原体，最初重点是埃博拉病毒。丝状病毒，如埃博拉病毒，是其中之一， 大多数致命的人类病原体，在疫情期间病死率很高。对于识别和 疫情管理是可以迅速和敏感地实施的强有力的检测方法。到 为了满足这一关键需求，将利用回音壁模式（WGM）传感器。WGM设备是一类 光被限制在微米级体积内的光学传感器。这些设备具有令人难以置信的高 灵敏度高、传感器占地面积小、易于与传统电子器件集成以及制造成本低。 微泡谐振器（MBRs）是WGM传感器的一个子类，具有传统WGM传感器的优点 同时能够将光学部件和流体部件耦合到单个部件中。这个目标 该提案是使用基于MBR的光流体传感器平台来快速、多路检测埃博拉病毒。 工作流程和进展，包括MBR开发，工程抗体，生物物理验证 和多路复用，将提供一个框架，以扩展工作超出最初的目标，并促进容易 为卡维博士的独立事业做好准备