Development of Optofluidic Resonators for Filoviral Detection

用于丝状病毒检测的光流控谐振器的开发

• 批准号: 10668494
• 负责人: Abraham Jaleel Qavi
• 金额: $ 18.97万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10668494
• 关键词: 2019-nCoV; Address; Affinity; Africa; Africa South of the Sahara; Area; Binding; Biochemistry; Biological Assay; Biophysics; Biosensing Techniques; Buffers; COVID-19 pandemic; California; Case Fatality Rates; Cessation of life; Chemistry; Clinical Pathology; Cold Chains; Communicable Diseases; Complex; Coupling; Data; Democratic Republic of the Congo; Detection; Development; Development Plans; Devices; Diagnostic; Disease; Disease Outbreaks; Doctor of Philosophy; Ebola; Ebola Hemorrhagic Fever; Ebola virus; Electronics; Engineering; Enrollment; Enzyme-Linked Immunosorbent Assay; Filovirus; Foundations; Glycoproteins; Goals; Hospitals; Illinois; Immune response; Immunology; Individual; Infection; International; Laboratories; Lasers; Lassa virus; Lead; Light; Liquid substance; Measurement; Medical; Mentors; Mentorship; Methods; Microbubbles; Molecular Biology; Monoclonal Antibodies; Optics; Output; Pathogen detection; Pathogenicity; Pathology; Pathway interactions; Performance; Phase; Physicians; Positioning Attribute; Process; Protein Biochemistry; Protein Engineering; RNA Viruses; Reagent; Reporter; Research; Research Personnel; Residencies; Sampling; Scholars Program; Scientist; Sentinel; Serology; Signal Transduction; Source; System; Techniques; Testing; Time; Training; Universities; Validation; Vesicular stomatitis Indiana virus; Viral; Viral Genome; Viral Hemorrhagic Fevers; Viral Markers; Viral Proteins; Virus; Virus Diseases; Washington; Work; analytical tool; antibody engineering; antibody mimetics; career; career development; design; detection method; diagnostic assay; emerging pathogen; follower of religion Jewish; human pathogen; manufacturing cost; multiplex detection; nanoparticle; new outbreak; novel; novel diagnostics; optical sensor; particle; pathogen; photonics; physician-scientist training program; professor; programs; rapid detection; response; sensor; sensor technology; standard of care; training opportunity; viral detection; virology

Project Summary/Abstract This proposal describes a 4-year career development plan for the PI, Dr. Abraham J. Qavi, MD, PhD, with the goal of preparing him for an independent research career as a physician scientist. This plan includes expand training opportunities and a pathway to an independent career that includes the development and implementation of sensor technologies and its application towards filoviruses, namely Ebola. The PI graduated with degrees in Biochemistry & Molecular Biology and Chemistry from the University of California, Irvine. He then enrolled in the Medical Scholars Program at the University of Illinois at Urbana-Champaign, where he earned his MD and PhD in Chemistry. Dr. Qavi continued his medical training as part of the Clinical Pathology Physician Scientist Training Program at Washington University in St. Louis and the Barnes-Jewish Hospital consortium. During his residency elective time, he began research in the laboratory of Dr. Lan Yang, who will serve as his co-mentor together with Dr. Amarasinghe. Dr. Yang is the Edwin H. and Florence G. Skinner Professor of Electrical & Systems Engineering, and an internationally renowned researcher in photonics. In 2019, Dr. Qavi added an additional training component under the co-mentorship with Dr. Gaya Amarasinghe, Professor of Pathology & Immunology, to expand the application of his previously developed sensor technology to infectious disease reach. The goal of this study is the development of a rapid, multiplexed optofluidic sensor platform for the rapid detection of pathogens, with an initial focus on Ebola. Filoviruses, such as Ebola, are among the most lethal human pathogens, with high case fatality rates during outbreaks. Critical in the identification and management of outbreaks are robust detection methods that can be implemented rapidly and sensitively. To address this critical need, Whispering Gallery Mode (WGM) sensors will be leveraged. WGM devices are a class of optical sensors in which light is confined within a micron-scale volume. These devices have incredibly high sensitivity, small sensor footprint, ease of integration with conventional electronics, and low fabrication costs. Microbubble resonators (MBRs), a subclass of WGM sensors, offer the advantages of conventional WGM devices while enabling coupling of optical and the fluidic components into a single component. The goal of this proposal is the use of an optofluidic sensor platform based on MBRs for the rapid, multiplexed detection of Ebola. The workflow and advancements, including MBR development, engineered antibodies, biophysical validation and multiplexing, will provide the framework to extend the work beyond the initial goals and promote facile transition to an independent career for Dr. Qavi.

项目概要/摘要 该提案描述了 PI（医学博士、博士）Abraham J. Qavi 博士的 4 年职业发展计划，以及 目标是为他作为一名医师科学家的独立研究生涯做好准备。该计划包括扩大 培训机会和独立职业之路，包括开发和实施 传感器技术及其在丝状病毒（即埃博拉病毒）中的应用。 PI 毕业并获得学位 加州大学欧文分校的生物化学与分子生物学和化学。随后他报名参加了 伊利诺伊大学厄巴纳-香槟分校医学学者计划，他在那里获得医学博士和博士学位 在化学中。 Qavi 博士继续接受医学培训，作为临床病理学医师科学家培训的一部分 圣路易斯华盛顿大学和巴恩斯犹太医院联盟的项目。在他居住期间 选修时间，他开始在杨兰博士的实验室进行研究，杨兰博士将与他一起担任他的共同导师 阿马拉辛格博士。杨博士是 Edwin H. 和 Florence G. Skinner 电气与系统教授 工程学，国际知名的光子学研究员。 2019 年，Qavi 博士又添加了一个 与病理学和免疫学教授 Gaya Amarasinghe 博士共同指导下的培训部分， 将他之前开发的传感器技术的应用范围扩大到传染病领域。 本研究的目标是开发一种快速、多路复用光流控传感器平台，用于 快速检测病原体，最初重点关注埃博拉病毒。丝状病毒，例如埃博拉病毒，属于 最致命的人类病原体，爆发期间病死率很高。对于识别和识别至关重要 疫情管理是可以快速、灵敏地实施的强有力的检测方法。到 为了满足这一关键需求，将利用回音壁模式 (WGM) 传感器。 WGM 设备是一类 光被限制在微米级体积内的光学传感器。这些设备具有令人难以置信的高 灵敏度高、传感器占地面积小、易于与传统电子设备集成以及制造成本低。 微泡谐振器 (MBR) 是 WGM 传感器的一个子类，具有传统 WGM 的优点 装置，同时能够将光学和流体组件耦合到单个组件中。此举的目标 该提案是使用基于 MBR 的光流控传感器平台来快速、多重检测埃博拉病毒。 工作流程和进步，包括 MBR 开发、工程抗体、生物物理验证 和多路复用，将提供框架，将工作扩展到最初目标之外，并促进便利 Qavi 博士转向独立职业。