Center for Structural Biology of HIV RNA

核心2。晶体学 总结 CRNA晶体学核心将与CRNA研究人员合作，解决RNA的晶体结构， 蛋白质复合物，RNA和蛋白质。核心的一项主要活动是提供分子生物学方面的专门知识 为RNA和RNA-蛋白质复合物量身定制，包括构建体设计、表达测试和开发 的纯化方案，并在纯化的大分子和复合物的生物物理表征。这些 在分子生物学和生物物理学方面的贡献不仅对核心的晶体学重点至关重要， 但也可根据需要提供给CRNA项目，用于蛋白质或RNA-蛋白质的生产和表征 配合物核心还将提供构建设计，表达质粒和方案，以及纯化的 蛋白质、RNA和RNA-蛋白质复合物。密歇根大学的核心实验室将为 as a repository仓库for materials材料and protocols协议.核心团队由PI Janet Smith（密歇根大学）共同领导 和Jinwei Zhang（NIDDK，NIH），他带来了RNA和RNA-蛋白质复合物设计的专业知识， 晶化核心团队将专注于三个目标。核心将开发或调整尖端方法， 促进表达的RNA设计、RNA-蛋白质共表达、RNA的结晶分子伴侣，以及 建立体外RNA合成能力。为了支持其晶体学使命，内核将 开发尖端的方法来帮助RNA结晶，以提高衍射质量并解决相位 问题，以改善含RNA结构的晶体学细化，和 以促进晶体学和电子显微镜的结合。

Core 2. Crystallography Summary The CRNA Crystallography Core will collaborate with CRNA investigators to solve crystal structures of RNA- protein complexes, RNAs and proteins. A key activity of the Core is to provide expertise in molecular biology tailored for RNAs and RNA-protein complexes, including construct design, expression testing, and development of purification protocols, and in biophysical characterization of purified macromolecules and complexes. These contributions in molecular biology and biophysics are not only critical to the crystallography focus of the Core, but are also available to CRNA Projects as needed for production and characterization of proteins or RNA-protein complexes. The Core will also provide construct designs, expression plasmids and protocols as well as purified proteins, RNAs and RNA-protein complexes to CRNA labs. The Core lab at the University of Michigan will serve as a repository for materials and protocols. The Core team is co-led by PIs Janet Smith (University of Michigan) and Jinwei Zhang (NIDDK, NIH), who brings expertise in the design of RNAs and RNA-protein complexes for crystallization. The Core team will focus on three aims. The Core will develop or adapt cutting-edge methods for RNA designs to facilitate expression, RNA-protein co-expression, crystallization chaperones for RNA, and establishment of an in vitro RNA synthesis capability. In support of its crystallography mission, the Core will develop cutting-edge methods to aid RNA crystallization, to improve the diffraction quality and solve the phase problem for RNA-containing crystals, to improve crystallographic refinement for RNA-containing structures, and to facilitate the combination of crystallography and electron microscopy.