Center for Structural Biology of HIV RNA

HIV RNA结构生物学中心

• 批准号: 10505790
• 负责人: ALICE TELESNITSKY
• 金额: $ 37.39万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-09 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10505790
• 关键词: Biophysics; Budgets; Cell Line; Collaborations; Complex; Cryoelectron Microscopy; Crystallization; Crystallography; Development; Development Plans; Electron Microscopy; HIV; Human Resources; In Vitro; Lead; Location; Methods; Michigan; Mission; Molecular Biology; Molecular Chaperones; National Institute of Diabetes and Digestive and Kidney Diseases; Phase; Plasmids; Production; Proteins; Protocols documentation; RNA; RNA chemical synthesis; RNA purification; Research Personnel; Resolution; Role; Scientist; Services; Structure; Techniques; Testing; United States National Institutes of Health; Universities; X-Ray Crystallography; biophysical properties; design; design and construction; improved; macromolecule; member; protein complex; protein purification; repository; structural biology; technology development; tool

Core 2. Crystallography Summary The CRNA Crystallography Core will collaborate with CRNA investigators to solve crystal structures of RNA- protein complexes, RNAs and proteins. A key activity of the Core is to provide expertise in molecular biology tailored for RNAs and RNA-protein complexes, including construct design, expression testing, and development of purification protocols, and in biophysical characterization of purified macromolecules and complexes. These contributions in molecular biology and biophysics are not only critical to the crystallography focus of the Core, but are also available to CRNA Projects as needed for production and characterization of proteins or RNA-protein complexes. The Core will also provide construct designs, expression plasmids and protocols as well as purified proteins, RNAs and RNA-protein complexes to CRNA labs. The Core lab at the University of Michigan will serve as a repository for materials and protocols. The Core team is co-led by PIs Janet Smith (University of Michigan) and Jinwei Zhang (NIDDK, NIH), who brings expertise in the design of RNAs and RNA-protein complexes for crystallization. The Core team will focus on three aims. The Core will develop or adapt cutting-edge methods for RNA designs to facilitate expression, RNA-protein co-expression, crystallization chaperones for RNA, and establishment of an in vitro RNA synthesis capability. In support of its crystallography mission, the Core will develop cutting-edge methods to aid RNA crystallization, to improve the diffraction quality and solve the phase problem for RNA-containing crystals, to improve crystallographic refinement for RNA-containing structures, and to facilitate the combination of crystallography and electron microscopy.

核心 2. 晶体学 概括 CRNA 晶体学核心将与 CRNA 研究人员合作解决 RNA 的晶体结构- 蛋白质复合物、RNA 和蛋白质。核心的一项关键活动是提供分子生物学方面的专业知识 专为 RNA 和 RNA-蛋白质复合物量身定制，包括构建设计、表达测试和开发 纯化方案以及纯化大分子和复合物的生物物理表征。这些 在分子生物学和生物物理学方面的贡献不仅对核心的晶体学焦点至关重要， 但也可根据需要用于 CRNA 项目，用于蛋白质或 RNA 蛋白质的生产和表征 复合物。核心还将提供构建设计、表达质粒和方案以及纯化的 蛋白质、RNA 和 RNA-蛋白质复合物到 CRNA 实验室。密歇根大学的核心实验室将服务 作为材料和协议的存储库。核心团队由 PI Janet Smith（密歇根大学）共同领导 和 Jinwei Zhang（NIDDK、NIH），他带来了 RNA 和 RNA-蛋白质复合物设计方面的专业知识 结晶。核心团队将专注于三个目标。核心将开发或采用尖端方法 RNA 设计可促进表达、RNA-蛋白质共表达、RNA 结晶伴侣，以及 建立体外RNA合成能力。为了支持其晶体学使命，核心将 开发尖端方法来帮助 RNA 结晶、提高衍射质量并解决相位问题 解决含 RNA 晶体的问题，改善含 RNA 结构的晶体学细化，以及 促进晶体学和电子显微镜的结合。