African-American Susceptibility to Periodontal Disease due to Electronic Nicotine Delivery Systems (ENDS) Usage

项目摘要 非裔美国人（AA）接受和使用电子尼古丁输送系统（ENDS） 社区可能会扩大与烟草有关的健康差距，如牙龈炎症（GI）， 牙周病然而，使用ENDS如何以及为什么会造成GI风险尚不清楚，因为缺乏 关于AA ENDS使用者吸烟行为、暴露和疾病结局的综合数据。我们 假设AA ENDS使用者可能更容易受到ENDS介导的口腔健康结果， ENDS偏好，使用和生物反应的变化。为了解决这个问题，我们将建立一个 行为-暴露-毒性-疾病范式，利用专门设计的目标来评估行为（目标1）， 常见的ENDS产品（目标2）和易感性（目标3）。这种创新的方法将有助于 确定可应用于疾病预防策略的风险因素和生物标志物。 在目标1中，我们将招募目前AA和高加索ENDS使用者以及从不吸烟者的队列， 评估ENDS使用行为、口腔健康和风险因素的模式。为此，我们将采用 问卷调查和移动的生态瞬时评估，再加上实时监测， 结束每组内的抽吸地形图。通过使用这种混合方法，我们将限制回忆偏差， 还捕捉可能在这两个ENDS用户组中不同地发生的瞬间现象。到 阐明特定群体和基于产品的ENDS暴露场景的作用，我们将利用体外空气 Aim 2中的液界面细胞模型系统。使用特定的群体暴露情景（通过抽吸识别 目标1中的拓扑表征）和比较毒性评估的既定方案，我们将 识别与ENDS气溶胶暴露相关的差异生物标志物。具体而言，这将包括 比较不同ENDS代产品和品牌以及使用方法诱导的细胞反应 模式.在目标3中，我们将利用分子流行病学方法来评估差异化 通过使用参与者唾液和牙龈细胞来识别我们的AA和高加索人ENDS队列中的暴露， 氧化应激的生物标志物，DNA损伤和其他常见于GI的指标。具体来说，我们的目标是 建立ENDS偏好、抽吸地形和导致GI的毒理学途径之间的途径 可能在AA人群中加剧的发展。我们的项目成果将是一个生物标志物小组， 可能为AA中ENDS介导的GI易感性提供重要见解。

Project Summary The acceptance and use of electronic nicotine delivery systems (ENDS) within the African-American (AA) community may widen tobacco-related health disparities such as gingival inflammation (GI), the precursor to periodontal disease. However, how and why ENDS use may pose GI risk is unclear as there is a lack of comprehensive data on smoking behaviors, exposure, and disease outcomes in AA ENDS users. We hypothesize that AA ENDS users may be more susceptible to ENDS-mediated oral health outcomes due to variations in ENDS preference, usage, and biological responses. To address this hypothesis, we will establish a behavior-exposure-toxicity-disease paradigm utilizing aims specifically designed to evaluate behaviors (Aim 1), common ENDS products (Aim 2), and susceptibility (Aim 3). This innovative approach will facilitate the identification of risk factors and biomarkers that can be applied to disease prevention strategies. In Aim 1, we will recruit cohorts of current AA and Caucasian ENDS users and never-tobacco users to evaluate patterns of ENDS usage behaviors, oral health, and risk factors. In this aim, we will employ questionnaires and mobile ecological momentary assessments coupled with real-time monitoring to examine ENDS puff topography within each group. By using this mixed methods approach, we will limit recall bias and also capture moment to moment phenomena that may occur differentially in these two ENDS user groups. To elucidate the role of specific group and product-based ENDS exposure scenarios we will utilize an in vitro air liquid interface cellular model system in Aim 2. Using specific group exposure scenarios (identified by puff topography characterization in Aim 1) and established protocols for comparative toxicity assessments we will identify differential biomarkers associated with ENDS aerosol exposures. Specifically, this will include comparisons of cellular responses induced by differing ENDS generation products and brands as well as usage patterns. In Aim 3, we will utilize a molecular epidemiology approach to assess the impact of differential exposures in our AA and Caucasian ENDS cohorts by using participant saliva and gingival cells to identify biomarkers of oxidative stress, DNA damage, and other indicators commonly found in GI. Specifically, we aim to establish a pathway between ENDS preference, puff topography, and toxicological pathways contributing GI development that may be exacerbated in the AA population. Our project outcomes will be a biomarker panel that may provide critical insight into ENDS-mediated GI susceptibility in AA.