African-American Susceptibility to Periodontal Disease due to Electronic Nicotine Delivery Systems (ENDS) Usage

项目摘要 非裔美国人(AA)对电子尼古丁传递系统的接受和使用(完) 社区可能会扩大与烟草相关的健康差距，如牙周炎(GI)，这是 牙周病。然而，终端使用如何以及为什么可能构成胃肠道疾病风险尚不清楚，因为缺乏 关于AA最终用户的吸烟行为、暴露和疾病后果的综合数据。我们 假设AA终端用户可能更容易受到END中介的口腔健康结果的影响，因为 末端偏好、用途和生物反应的变化。为了解决这一假设，我们将建立一个 利用专门设计用于评估行为的目标的行为-暴露-毒性-疾病范例(目标1)， 共同终端产品(目标2)和易感性(目标3)。这种创新的方法将促进 确定可应用于疾病预防策略的风险因素和生物标志物。 在目标1中，我们将招募一批目前的AA和高加索最终用户和从不吸烟的用户 评估终端使用行为、口腔健康和风险因素的模式。为了达到这一目标，我们将采用 问卷调查和移动生态瞬时评估与实时监测相结合，以审查 结束每个组内的膨胀地形。通过使用这种混合方法，我们将限制召回偏见和 还可以捕捉在这两个终端用户组中可能出现的不同现象。至 阐明特定组和基于产品的末端暴露情景的作用，我们将利用体外空气 AIM中的液体界面细胞模型系统2.使用特定的组暴露场景(由Puff识别 目标1)中的地形特征和建立的比较毒性评估方案，我们将 确定与结束气溶胶暴露相关的差异生物标记物。具体而言，这将包括 不同终端、代产品、品牌和用法引起的细胞反应的比较 模式。在目标3中，我们将利用分子流行病学方法来评估差异的影响。 在我们的再生障碍性贫血和高加索人群中，暴露通过使用参与者唾液和牙龈细胞来识别 氧化应激、DNA损伤的生物标记物和其他在胃肠道疾病中常见的指标。具体来说，我们的目标是 在末端偏好、泡腾地形和毒理学途径之间建立一条导致GI的途径 在再生障碍性贫血人群中可能会加剧的发育。我们的项目成果将是一个生物标志物小组 可能对再生障碍性贫血中END介导的胃肠道易感性提供关键的洞察力。

Project Summary The acceptance and use of electronic nicotine delivery systems (ENDS) within the African-American (AA) community may widen tobacco-related health disparities such as gingival inflammation (GI), the precursor to periodontal disease. However, how and why ENDS use may pose GI risk is unclear as there is a lack of comprehensive data on smoking behaviors, exposure, and disease outcomes in AA ENDS users. We hypothesize that AA ENDS users may be more susceptible to ENDS-mediated oral health outcomes due to variations in ENDS preference, usage, and biological responses. To address this hypothesis, we will establish a behavior-exposure-toxicity-disease paradigm utilizing aims specifically designed to evaluate behaviors (Aim 1), common ENDS products (Aim 2), and susceptibility (Aim 3). This innovative approach will facilitate the identification of risk factors and biomarkers that can be applied to disease prevention strategies. In Aim 1, we will recruit cohorts of current AA and Caucasian ENDS users and never-tobacco users to evaluate patterns of ENDS usage behaviors, oral health, and risk factors. In this aim, we will employ questionnaires and mobile ecological momentary assessments coupled with real-time monitoring to examine ENDS puff topography within each group. By using this mixed methods approach, we will limit recall bias and also capture moment to moment phenomena that may occur differentially in these two ENDS user groups. To elucidate the role of specific group and product-based ENDS exposure scenarios we will utilize an in vitro air liquid interface cellular model system in Aim 2. Using specific group exposure scenarios (identified by puff topography characterization in Aim 1) and established protocols for comparative toxicity assessments we will identify differential biomarkers associated with ENDS aerosol exposures. Specifically, this will include comparisons of cellular responses induced by differing ENDS generation products and brands as well as usage patterns. In Aim 3, we will utilize a molecular epidemiology approach to assess the impact of differential exposures in our AA and Caucasian ENDS cohorts by using participant saliva and gingival cells to identify biomarkers of oxidative stress, DNA damage, and other indicators commonly found in GI. Specifically, we aim to establish a pathway between ENDS preference, puff topography, and toxicological pathways contributing GI development that may be exacerbated in the AA population. Our project outcomes will be a biomarker panel that may provide critical insight into ENDS-mediated GI susceptibility in AA.