Assessment of Cured-In-Place Pipe Installation Emissions and Toxicity

项目摘要 在全国和全球范围内，对卫生下水道、雨水下水道和饮用水的维修需求不断增长 管.现场固化管道（CIPP）法已成为修复水质恶化的最常用方法 管.该过程涉及在管道内现场化学制造聚合物复合塑料管， 管道损坏。最近的证据表明，公共卫生事件与化学品空气污染有关 从CIPP站点发出。这些事件跨越29个州，并促使办公楼撤离， 家庭和学校我们最近对CIPP工地的评估发现， 有害的空气污染物，致癌物质，内分泌干扰化合物和其他正在释放到 环境保护此外，我们对排放相关毒性的初步体外检查表明， 表明CIPP操作程序影响的具体答复。具体来说， 通过细胞毒性的改变以及细胞毒性标志物中基因和蛋白质表达的变化来确定。 炎症和氧化应激。树脂的种类、固化方法和环境条件 沿着工作场所的瞬态特性，影响执行系统排放评估的能力 和毒性。因此，我们生产了一种新型的固化室来表征CIP相关的排放， 检查不良的生物反应。本提案的中心目标是调查CIPP的运作情况， 影响排放和毒性的程序。我们的假设是，通常使用的树脂材料 （苯乙烯基或非苯乙烯基）在固化过程中产生不同的排放， 吸入后的毒性。为了解决我们的假设，我们提出了以下具体目标：1。评价 由于树脂材料的差异CIP相关的发射曲线，和2.评估对健康的不利影响 在吸入暴露于CIP排放物之后。在目标1中，用苯乙烯或非苯乙烯浸渍的管 基树脂将在固化室内进行热固化， 光电离检测器和气相色谱-质谱法。在目标2中，将雄性和雌性小鼠 暴露于过滤空气（对照）、CIPP排放物或仅苯乙烯，并检查肺和肝毒性 （炎症和氧化应激）以及血清代谢物的改变。在该项目完成时， 我们期望我们已经开始阐明CIPP树脂材料、排放 特征和毒性。最终，这项研究的长期影响将是监管风险的潜力， 通过界定安全操作程序，减少公众接触CIPP排放物。

Project Abstract Nationwide and globally there is a growing need for repair of sanitary sewer, storm sewer, and drinking water pipes. The cured-in-place pipe (CIPP) method has become the most popular method to repair deteriorated water pipes. This process involves the onsite chemical fabrication of a polymer composite plastic pipe within the damaged pipe. Recent evidence has indicated public health incidences related to chemical air contamination emitted from CIPP sites. These incidents span 29 states and have prompted evacuations of office buildings, homes, and schools. Our recent evaluations of CIPP worksites have identified emissions containing numerous hazardous air pollutants, carcinogens, endocrine disrupting compounds, and others that are being released into the environment. Further, our preliminary in vitro examination of emission-related toxicity demonstrated site- specific responses suggesting the impact of CIPP operational procedures. Specifically, the differential toxicity was determined via alterations in cytotoxicity, as well as gene and protein expression changes in markers of inflammation and oxidative stress. The multitude of resins, curing approaches, and environmental conditions along with the transient nature of worksites, impacts the ability to perform systematic evaluation of emissions and toxicity. Therefore, we have produced a novel curing chamber to characterize CIPP-related emissions and examine adverse biological responses. The central objective of this proposal is to investigate CIPP operational procedures that influence emissions and toxicity. Our hypothesis is that commonly utilized resin materials (styrene or non-styrene based) produce distinct emissions during the curing process that result in differential toxicity following inhalation. To address our hypothesis, we propose the following specific Aims: 1. Evaluation of differential CIPP-related emission profiles due to resin materials, and 2. Assessment of adverse health effects following inhalation exposure to CIPP-emissions. In Aim 1, tubing impregnated with either styrene or non-styrene based resin will be thermally cured within the curing chamber and emissions will be characterized via photoionization detectors and gas chromatography-mass spectrometry. In Aim 2, male and female mice will be exposed to filtered air (controls), CIPP emissions, or styrene-only and examined for pulmonary and liver toxicity (inflammation and oxidative stress) as well as alterations in serum metabolites. At the completion of this project, it is our expectation that we will have begun to elucidate the relationship between CIPP resin materials, emission profiles, and toxicity. Ultimately, the long-term impact of this research will be the potential to regulate risk, related to CIPP emission exposures by the general public through defining safe operational procedures.