Assessment of Cured-In-Place Pipe Installation Emissions and Toxicity

项目摘要 在全国和全球范围内，对卫生下水道、雨水下水道和饮用水的维修需求日益增长 管子。就地固化管道(CIPP)法已成为修复劣化水最受欢迎的方法 管子。这一过程包括在现场化学制造聚合物复合塑料管 管道损坏。最近的证据表明，与化学空气污染有关的公共卫生事件 从CIPP站点排放。这些事件横跨29个州，促使办公楼疏散， 房子和学校。我们最近对CIPP工作场所的评估发现，排放中含有大量 有害的空气污染物、致癌物、内分泌干扰化合物和其他正在被释放到 环境。此外，我们对排放相关毒性的初步体外检测表明- 具体回应建议CIPP业务程序的影响。具体地说，区别毒性 是通过细胞毒性的变化以及标志的基因和蛋白质表达的变化来确定的 炎症和氧化应激。树脂的种类、固化方法和环境条件 加上工作场所的瞬时性，影响了对排放进行系统评估的能力 和毒性。因此，我们生产了一种新型固化室来表征与CIPP相关的排放和 检查不良的生物反应。该提案的中心目标是调查CIPP的运行情况 影响排放和毒性的程序。我们的假设是常用的树脂材料 (苯乙烯或非苯乙烯基)在固化过程中会产生不同的排放，导致不同的 吸入后的毒性。为了解决我们的假设，我们提出了以下具体目标：1.评估 由树脂材料引起的与CIPP相关的不同排放概况，以及2.对健康不利影响的评估 在吸入接触CIPP排放物之后。在目标1中，用苯乙烯或非苯乙烯浸渍的管材 基树脂将在固化室内进行热固化，排放将通过 光电离检测器和气相色谱-质谱仪。在目标2中，雄性和雌性小鼠将被 暴露于过滤空气(对照)、CIPP排放或仅含苯乙烯的环境中，并检查其肺和肝脏毒性 (炎症和氧化应激)以及血清代谢物的改变。在这个项目完成时， 我们的期望是，我们将开始阐明CIPP树脂材料、排放物 个人资料和毒性。最终，这项研究的长期影响将是监管风险的潜力，相关 通过制定安全的操作程序，减少公众对CIPP排放的暴露。

Project Abstract Nationwide and globally there is a growing need for repair of sanitary sewer, storm sewer, and drinking water pipes. The cured-in-place pipe (CIPP) method has become the most popular method to repair deteriorated water pipes. This process involves the onsite chemical fabrication of a polymer composite plastic pipe within the damaged pipe. Recent evidence has indicated public health incidences related to chemical air contamination emitted from CIPP sites. These incidents span 29 states and have prompted evacuations of office buildings, homes, and schools. Our recent evaluations of CIPP worksites have identified emissions containing numerous hazardous air pollutants, carcinogens, endocrine disrupting compounds, and others that are being released into the environment. Further, our preliminary in vitro examination of emission-related toxicity demonstrated site- specific responses suggesting the impact of CIPP operational procedures. Specifically, the differential toxicity was determined via alterations in cytotoxicity, as well as gene and protein expression changes in markers of inflammation and oxidative stress. The multitude of resins, curing approaches, and environmental conditions along with the transient nature of worksites, impacts the ability to perform systematic evaluation of emissions and toxicity. Therefore, we have produced a novel curing chamber to characterize CIPP-related emissions and examine adverse biological responses. The central objective of this proposal is to investigate CIPP operational procedures that influence emissions and toxicity. Our hypothesis is that commonly utilized resin materials (styrene or non-styrene based) produce distinct emissions during the curing process that result in differential toxicity following inhalation. To address our hypothesis, we propose the following specific Aims: 1. Evaluation of differential CIPP-related emission profiles due to resin materials, and 2. Assessment of adverse health effects following inhalation exposure to CIPP-emissions. In Aim 1, tubing impregnated with either styrene or non-styrene based resin will be thermally cured within the curing chamber and emissions will be characterized via photoionization detectors and gas chromatography-mass spectrometry. In Aim 2, male and female mice will be exposed to filtered air (controls), CIPP emissions, or styrene-only and examined for pulmonary and liver toxicity (inflammation and oxidative stress) as well as alterations in serum metabolites. At the completion of this project, it is our expectation that we will have begun to elucidate the relationship between CIPP resin materials, emission profiles, and toxicity. Ultimately, the long-term impact of this research will be the potential to regulate risk, related to CIPP emission exposures by the general public through defining safe operational procedures.