Determining the Role of Extracellular Matrix Compliance and Composition on Facial Morphogenesis

确定细胞外基质顺应性和成分对面部形态发生的作用

• 批准号: 10516742
• 负责人: Nicholas Jean Hanne
• 金额: $ 7.46万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10516742
• 关键词: 3-Dimensional; Actomyosin; Affect; Agreement; Area; Atomic Force Microscopy; Basement membrane; Cell Polarity; Cell Proliferation; Cell Shape; Cells; Cleft Palate; Cleft lip with or without cleft palate; Collagen; Confocal Microscopy; Congenital Abnormality; Craniofacial Abnormalities; DNA Sequence Alteration; Data; Development; Dysmorphology; Ectoderm; Embryo; Environment; Enzymes; Epithelium; Etiology; Exposure to; Extracellular Matrix; Extracellular Matrix Proteins; Face; Failure; Family; Feedback; Genes; Genetic; Goals; Growth; Growth Factor; Human; Knock-out; Laminin; Lathyrus; Limb Bud; Maps; Measures; Medial; Mesenchymal; Mesenchyme; Modeling; Molecular; Morphogenesis; Morphology; Movement; Mus; Mutation; Nose; Outcome Measure; Parents; Patients; Premaxillary palate; Primordium; Process; Proliferating; Property; Protein-Lysine 6-Oxidase; Publishing; Research; Role; S-Phase Fraction; Shapes; Signal Transduction; Spectroscopy, Fourier Transform Infrared; Structure; Surface; Testing; Thick; Thinness; Tissues; Transmission Electron Microscopy; Work; Zebrafish; cell behavior; cell growth; cell motility; craniofacial; crosslink; interest; member; physical property; prevent; regional difference; response; scaffold; small molecule

PROJECT SUMMARY Facial morphogenesis involves directed outgrowth of the facial primordia until they appose and fuse with one another. Currently, outgrowth of primordia is considered to be driven primarily by bulk tissue displacement caused by proliferation gradients in the mesenchymal tissue. However, our lab and others have found that other mechanisms, such as directed cellular migration and epithelium guided growth, likely contribute as well. In the limb bud, tensile forces generated by the ectoderm have been shown to direct cellular orientation and growth, and proliferation gradients across the limb bud cannot accurately model morphogenesis without incorporating directed cellular behaviors. Our lab has previously shown that mesenchymal cells in the face are polarized and that activation of fibroblastic growth factor (FGF) signaling disrupts this polarity while causing aberrant outgrowth of the primordium. The extracellular matrix (ECM) around cells provides the structural scaffolding required to generate and guide force through tissue, and the ECM in the epithelium may guide or constrain growth regionally. I hypothesize that the ECM directs morphogenesis in the face via regional differences in composition and compliance. I will test this hypothesis with two main aims. In the first aim I will examine how FGF signaling affects ECM physical properties and cellular organization in the facial primordium. I will quantitatively map the composition of ECM proteins and ECM compliance across the face with confocal microscopy and atomic force microscopy, then relate regional differences in these measures to facial shape change. This aim will elucidate mechanisms by which molecular signaling of fibroblastic growth factor controls morphogenesis through the ECM and cellular organization. In the second aim, I will increase or decrease tissue compliance directly by altering collagen crosslinking, then repeat the same analyses as the first aim. The second aim will determine if ECM stiffness directs outgrowth directly while the mesenchyme passively conforms around it, or if there is feedback between ECM and cells that actively remodel the environment to direct growth. Failure of the facial primordium to appose and fuse leads to facial clefting. Understanding the role of ECM in directional growth is important for understanding the etiology of birth defects in the face.

项目总结