How does glycosylation shape protein function within Burkholderia?

糖基化如何塑造伯克霍尔德杆菌内蛋白质的功能？

• 批准号: FT200100270
• 负责人: A/Prof Nichollas Scott
• 金额: $ 60.3万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT200100270
• 关键词: How; does; glycosylation; shape; protein

Protein glycosylation, the chemical addition of sugars to proteins, is an important but poorly understood aspect of bacterial physiology. This project aims to build on our recent discovery of the conservation of O-linked glycosylation across the Burkholderia genus to understand the function of this modification. Using cutting-edge proteomics, novel expression systems and molecular approaches this project will reveal the role of glycosylation in Burkholderia species. This innovative project will provide a comprehensive understanding of how glycosylation contributes to Burkholderia protein function and how these systems can be harnessed for the creation of bespoke glycoconjugates

蛋白质糖基化，即糖与蛋白质的化学加成，是细菌生理学的一个重要但知之甚少的方面。该项目旨在建立在我们最近发现的O-连接糖基化在伯克霍尔德氏菌属中的保守性的基础上，以了解这种修饰的功能。利用尖端的蛋白质组学，新的表达系统和分子方法，该项目将揭示糖基化在伯克霍尔德菌属物种中的作用。这个创新项目将提供一个全面的了解如何糖基化有助于伯克霍尔德氏菌蛋白质的功能，以及如何利用这些系统来创建定制的糖缀合物