Characterising O-linked glycosylation across Burkholderia

伯克霍尔德杆菌中 O-连接糖基化的表征

• 批准号: DP210100362
• 负责人: A/Prof Nichollas Scott
• 金额: $ 37.62万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-03-23 至 2024-04-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210100362
• 关键词: Characterising; linked; glycosylation; across; Burkholderia

Protein glycosylation, the chemical addition of sugars to proteins, enables the augmentation of protein properties. Across the Burkholderia genus we have shown O-linked glycosylation is both conserved as well as essential for bacterial fitness. Yet, we have little understanding of how glycosylation modulates the proteome of this genus. This project aims to characterise the glycoproteomes of Burkholderia species and track the impact of glycosylation on both the proteome and protein stability. By understanding how glycosylation shapes the proteome we will gain a greater understanding of the role of bacterial glycosylation in Burkholderia physiology as well as how we may better utilise microbial glycosylation for glycoprotein production.

蛋白质糖基化，即糖与蛋白质的化学加成，能够增强蛋白质的性质。在伯克霍尔德氏菌属中，我们已经表明O-连接的糖基化是保守的，也是细菌适应性所必需的。然而，我们对糖基化如何调节这一属的蛋白质组知之甚少。该项目旨在对伯克霍尔德菌属物种的糖蛋白质组进行测序，并跟踪糖基化对蛋白质组和蛋白质稳定性的影响。通过了解糖基化如何塑造蛋白质组，我们将更好地了解细菌糖基化在伯克霍尔德氏菌生理学中的作用，以及我们如何更好地利用微生物糖基化生产糖蛋白。