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Elucidating the Role of Dynamic X-Chromosome Inactivation Maintenance in the Pathogenesis of Systemic Sclerosis

阐明动态 X 染色体失活维持在系统性硬化症发病机制中的作用

基本信息

批准号：
10511513
负责人：
Montserrat C Anguera
金额：
$ 21.45万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-12 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10511513
关键词：
Affect Attention Autoimmune Diseases Bleomycin CD3 Antigens CD8-Positive T-Lymphocytes CREST Syndrome CXCR3 gene Candidate Disease Gene Cell physiology Cells Cicatrix Collagen Cutaneous Cytology Data Defect Development Disease Embryonic Development Epigenetic Process Exhibits FOXP3 gene Female Fibrosis Fluorescent in Situ Hybridization Future Gene Dosage Gene Expression Gene Expression Profile Gene Expression Regulation Gene Silencing Genes Genetic Risk Genetic Transcription Goals Harvest Heterochromatin Hormonal Human IL13RA1 gene Immune Immune system Immunofluorescence Immunologic Impairment In Vitro Inflammation Investigation Knockout Mice Laboratories Link Lupus Lymphocyte Maintenance Mediating Modeling Modification Molecular Monozygotic twins Morbidity - disease rate Mus Nuclear Organ Outcome Pathogenesis Pathogenicity Pathology Patients Pattern Peripheral Predisposition Process Pulmonary Fibrosis RNA RNA Interference Rest Role Scleroderma Serum Sex Bias Skin Skin Tissue Somatic Cell Splenic Tissue Structure of parenchyma of lung Systemic Scleroderma T-Lymphocyte T-Lymphocyte Subsets TIMP1 gene TNFSF5 gene Testing Therapeutic Therapeutic Intervention Tissues Untranslated RNA Vascular Diseases Wild Type Mouse Woman Work X Chromosome X Inactivation base biological sex cell type cellular imaging comparative epigenetic regulation epigenetic silencing histone modification immune function improved insight male men mortality mouse model novel novel therapeutics overexpression peripheral blood prevent programs recruit risk variant single molecule skin fibrosis subcutaneous systemic autoimmune disease systemic autoimmunity transcriptome transcriptome sequencing transcriptomics

项目摘要

Project Summary/Abstract: Systemic sclerosis (SSc) is a systemic autoimmune disease that predominantly affects women and is associated with significant morbidity and mortality due to fibrosis of the skin and internal organs. The molecular origin of this striking female bias and its relationship to core aspects of the pathogenesis of SSc, including immune dysregu- lation, multiorgan fibrosis, and vasculopathy, remains unclear. While there are known genetic risk variants asso- ciated with SSc, the low monozygotic twin concordance rate has prompted investigation into the role of epige- netic dysregulation in its pathogenesis. Importantly, the X-chromosome contains several proinflammatory and profibrotic genes, and their appropriate expression in females is epigenetically regulated in part through X-chro- mosome inactivation (XCI), a female-specific epigenetic mechanism that silences one of the two X chromosomes in order to equalize X-linked gene dosage between XX females and XY males. XCI is maintained in somatic cells by a variety of epigenetic modifications that are enriched on the inactive X chromosome (Xi), including the long noncoding RNA XIST and additional heterochromatic histone modifications. We recently discovered that XCI maintenance in T-cells from both humans and mice is “dynamic.” In “dynamic XCI maintenance”, XIST RNA and heterochromatic marks are cytologically absent from the Xi in resting T-cells and subsequently relocalize to the Xi upon in vitro cellular activation. We have found that dynamic XCI maintenance is impaired in T-cells from women with lupus, another female-biased autoimmune disease, and is associated with aberrant X-linked gene expression from the Xi. Based on the well-established role of T-cells in SSc-mediated pathology, the number of X-linked proinflammatory and profibrotic genes, the aberrantly increased expression of many of these genes in SSc T-cells, and our preliminary data, we hypothesize that impaired dynamic XCI maintenance in T-cells is a pathogenic mechanism in SSc that accordingly contributes to the observed female bias. In Aim 1, we will use single cell imaging to determine whether XCI maintenance is impaired in T-cells from females with SSc. We will couple these analyses with transcriptomic studies to determine the contribution of the Xi to the observed X-linked gene expression profile. In Aim 2, we will induce scleroderma-like disease in a novel mouse model of impaired XCI maintenance to determine how impaired XCI maintenance in T-cells impacts T-cell-specific transcriptional programs conferring susceptibility to fibrosis in SSc-relevant organs. Collectively, this work has the potential to identify a novel epigenetic mechanism of female bias in SSc and will simultaneously identify which X-linked genes become aberrantly expressed in T-cells during the development of fibrotic disease. These findings will improve our understanding of how biological sex contributes to the pathogenesis of SSc and related female- biased autoimmune disease and may enable the eventual discovery of new therapies for SSc.

项目总结/文摘: