CORE 3 - Quantitative Metabolism and Imaging Core

项目总结 定量代谢和成像核心将为营养和肥胖研究人员提供 研究新陈代谢的先进技术。将为核心用户提供靶向代谢组学、稳定同位素 代谢流量的示踪测量，以及成像方法。该中心还将与调查人员进行磋商 关于实验设计和数据解释。代谢学方法将被用来量化中介 利用基于代谢组学的靶向MS研究血浆或组织提取物中的代谢物和辅因子。因为这些 分析可以快速而廉价地进行，它们将被用作调查的第一线 通知设计更密集的助熔剂实验。稳定同位素示踪实验将提供详细的 关于整个通路功能的信息，通过使用结合到 下游代谢物。将使用核磁共振和/或MS检测到的代谢物的标记特性 以确定代谢物周转或进行代谢流量分析。数据将被返还给调查员 将就数据解释征求核心工作人员的意见。这些方法可用于以下项目 细胞培养、小鼠模型和人类受试者。通量研究可以与额外的分析相协调 使用体内成像光谱学技术的身体成分和组织细胞内脂肪含量。 使用~(13)C-可实现组织特异性代谢通量的额外代谢成像研究 超极化成像。定量代谢和成像核心提供了访问许多状态的途径- NORC调查人员开展严谨和创新的研究所需的最先进方法 营养与肥胖研究。

PROJECT SUMMARY The Quantitative Metabolism and Imaging Core will provide nutrition and obesity researchers access to advanced techniques to study metabolism. Core users will be provided targeted metabolomics, stable isotope tracer measurements of metabolic flux, and imaging approaches. The Core will also consult with investigators on experimental design and data interpretation. Metabolomic approaches will be used to quantify intermediary metabolites and co-factors in plasma or tissue extracts using targeted MS based metabolomics. Since these analyses can be performed quickly and inexpensively, they will be used as the first line of investigation to inform the design of more intensive flux experiments. Stable isotope tracer experiments will provide detailed information about the function of entire pathways by using labeled substrates that incorporate into downstream metabolites. Labeling characteristics of metabolites, detected by NMR and/or MS, will be used to determine metabolite turnover or perform metabolic flux analysis. Data will be returned to the investigator and core staff will be consulted on data interpretation. These approaches can be used in projects involving cell culture, mouse models and human subjects. Flux studies can be coordinated with additional analysis of body composition and tissue intracellular lipid content using in vivo imaging spectroscopy techniques. Additional metabolic imaging studies of tissue specific metabolic flux can be achieved using 13C- hyperpolarized imaging. The Quantitative Metabolism and Imaging Core provides access to many of the state- of-the-art approaches necessary for NORC investigators to carry out rigorous and innovative studies of nutrition and obesity research.