CORE 3 - Quantitative Metabolism and Imaging Core

CORE 3 - 定量代谢和成像核心

• 批准号: 10657785
• 负责人: Shawn C Burgess
• 金额: $ 16.4万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10657785
• 关键词: Animal Model; Biochemical Pathway; Body Composition; Carbon; Cell Culture Techniques; Characteristics; Consult; Data; Data Analyses; Dual-Energy X-Ray Absorptiometry; Experimental Designs; Image; Imaging Device; Investigation; Isotope Labeling; Label; Lipids; Measurement; Metabolic; Metabolic Pathway; Metabolic dysfunction; Metabolism; Obesity; Pathway interactions; Pattern; Plasma; Research; Research Personnel; Spectrum Analysis; Techniques; Tissue Extracts; Tissue imaging; Tissues; Tracer; cofactor; design; experimental study; human model; human subject; imaging approach; imaging study; in vivo imaging; innovation; mathematical model; metabolic abnormality assessment; metabolic imaging; metabolomics; mouse model; nutrition; spectroscopic imaging; stable isotope

PROJECT SUMMARY The Quantitative Metabolism and Imaging Core will provide nutrition and obesity researchers access to advanced techniques to study metabolism. Core users will be provided targeted metabolomics, stable isotope tracer measurements of metabolic flux, and imaging approaches. The Core will also consult with investigators on experimental design and data interpretation. Metabolomic approaches will be used to quantify intermediary metabolites and co-factors in plasma or tissue extracts using targeted MS based metabolomics. Since these analyses can be performed quickly and inexpensively, they will be used as the first line of investigation to inform the design of more intensive flux experiments. Stable isotope tracer experiments will provide detailed information about the function of entire pathways by using labeled substrates that incorporate into downstream metabolites. Labeling characteristics of metabolites, detected by NMR and/or MS, will be used to determine metabolite turnover or perform metabolic flux analysis. Data will be returned to the investigator and core staff will be consulted on data interpretation. These approaches can be used in projects involving cell culture, mouse models and human subjects. Flux studies can be coordinated with additional analysis of body composition and tissue intracellular lipid content using in vivo imaging spectroscopy techniques. Additional metabolic imaging studies of tissue specific metabolic flux can be achieved using 13C- hyperpolarized imaging. The Quantitative Metabolism and Imaging Core provides access to many of the state- of-the-art approaches necessary for NORC investigators to carry out rigorous and innovative studies of nutrition and obesity research.

项目摘要 定量代谢和成像核心将为营养和肥胖研究人员提供 研究新陈代谢的先进技术。核心用户将获得靶向代谢组学、稳定同位素 代谢通量的示踪剂测量和成像方法。核心还将与调查人员协商 关于实验设计和数据解释。代谢组学方法将用于定量中间体 使用基于靶向MS的代谢组学分析血浆或组织提取物中的代谢物和辅因子。由于这些 分析可以快速和廉价地进行，它们将被用作调查的第一线， 为设计更密集的通量实验提供信息。稳定同位素示踪实验将提供详细的 通过使用标记的底物， 下游代谢物。将使用通过NMR和/或MS检测的代谢物的标记特征 以确定代谢物周转或进行代谢通量分析。数据将返回给研究者 并会就数据的解释咨询核心人员。这些方法可用于涉及 细胞培养、小鼠模型和人类受试者。通量研究可以与以下附加分析相协调： 身体组成和组织细胞内脂质含量使用体内成像光谱技术。 可以使用13 C-12 C来实现组织特异性代谢通量的另外的代谢成像研究。 超极化成像定量代谢和成像核心提供了许多国家的访问- NORC研究人员进行严格和创新的研究所需的最先进的方法， 营养和肥胖研究。