ADAPTION TO CLIMATE CHANGE IN THE RHIZOSPHERE ACROSS THE MILLENNIA

千年来根际气候变化的适应

• 批准号: 10109155
• 金额: $ 65.6万
• 依托单位: HERIOT-WATT UNIVERSITY
• 依托单位国家: 英国
• 项目类别: EU-Funded
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10109155
• 关键词: ADAPTION; CLIMATE; CHANGE; RHIZOSPHERE; ACROSS

Starting from a unique collection of paleo-environmental samples (frozen Arctic soils and sediments) already at AWI and their corresponding ancient DNA (aDNA) metagenomes that stretch back up to a million years, we will retrieve information on how rhizosphere biodiversity and functionality responded to climate changes and extreme events. Preliminary metagenomic data from the collection suggests it is a gold mine of archaic DNA that represents a timeline of adaptions to climate change in the rhizosphere. By reconstructing and analyzing these ancient metagenomes and correlating with available historical climatic change data, we will identify molecular adaptions that impart climate tolerance (specifically resistance to increased temperature and drought). This will be used to i) produce and test engineered root-colonizing bacteria (Pseudomonas fluorescens and Pseudomonas chlororaphis) that will improve climate tolerance of plants (production of humidifying polysaccharides around the root) facilitating the ability to grow on marginal agricultural land (MAL), ii) inform ancestral reconstruction of thermostable and/or cold tolerant enzymes for industrial application and iii) produce engineered Pseudomonas putida strains tailored for bioproduction. For the latter application, we will select genes that encode biomolecules relevant for climate-tolerant phenotypes (humidifying polysaccharides and the biosurfactant betaines). The production of these molecules using biotechnology will be targeted in the project and their application in selected industrial products verified. Target end-user applications will include polysaccharides and betaines for the development of 3D printed organ-on-chip and drug delivery systems as well as the formulation of metalworking fluids, lubricants and industrial cleaning products.

从AWI已经收集的独特的古环境样本（冰冻的北极土壤和沉积物）及其相应的古代DNA（aDNA）宏基因组开始，追溯到一百万年前，我们将检索有关根际生物多样性和功能如何应对气候变化和极端事件的信息。初步的宏基因组数据表明，这是一个金矿的古代DNA，代表了时间轴的适应气候变化的根际。通过重建和分析这些古老的宏基因组，并与现有的历史气候变化数据相关联，我们将确定赋予气候耐受性（特别是对温度升高和干旱的抵抗力）的分子适应。这将用于i）生产和测试工程根定殖细菌（荧光假单胞菌和绿针假单胞菌），将提高植物的气候耐受性（在根部周围产生多糖）促进在边际农业用地（MAL）上生长的能力，ii）为工业应用提供耐热和/或耐寒酶的祖先重建信息，和iii）生产专为生物生产而设计的恶臭假单胞菌工程菌株。对于后一种应用，我们将选择编码与气候耐受表型相关的生物分子（多糖和生物表面活性剂甜菜碱）的基因。该项目将以利用生物技术生产这些分子为目标，并验证其在选定工业产品中的应用。目标最终用户应用将包括用于开发3D打印器官芯片和药物输送系统的多糖和甜菜碱，以及金属加工液，润滑剂和工业清洁产品的配方。