Zebrafish International Resource Center

总体项目摘要/摘要 斑马鱼是研究脊椎动物发育、生理、行为、遗传学和 疾病。强大的技术允许高效地产生和恢复斑马鱼突变，影响 调节发育模式、器官发生、生理和行为的基因。近期 研究转基因斑马鱼和反义斑马鱼基因功能的进展 寡核苷酸。这些基因中的许多(如果不是大部分)的功能在脊椎动物中是保守的 组。因此，对斑马鱼突变的分析提供了对其他脊椎动物基因功能的洞察， 包括人类。 斑马鱼国际资源中心已经建立，作为一个提供 向研究界提供动物、材料和服务。该提案寻求继续提供资金1) 作为斑马鱼遗传资源和研究材料的中央储存库，2)提供 咨询和病理服务，以及3)开发一个基于一组聚合酶链式反应分析的平台， 筛选实验室斑马鱼中最流行的病原体。这些材料、服务和 将向研究界广泛提供信息。 多样性补充摘要/摘要 我们申请了斑马鱼国际资源P40基金(OD011021)的多样性补充 中心(Zirc)为美国公民和Yakama Nation成员Corbin Jaid Schuster先生提供服务。这个 研究计划将Zirc P40赠款的目标2和3与科尔宾的长期职业目标联系在一起，即雇用 研究斑马鱼模式并开发类似野生和水产养殖鱼类的诊断筛查服务 为了Yakama Nation。反过来，ZIRC将受益于采用环境抽样方法和 DPCR技术，科尔宾在他的博士期间开发的。

Overall Project Summary/Abstract Zebrafish is a premiere organism to study vertebrate development, physiology, behavior, genetics and disease. Powerful techniques allow efficient generation and recovery of zebrafish mutations affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Recent advances make it easy to study gene function in transgenic zebrafish and with antisense oligonucleotides. The functions of many, if not most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene function in other vertebrates, including humans. The Zebrafish International Resource Center has been established as a repository that provides animals, materials and services to the research community. This proposal seeks continued funding 1) to serve as a central repository for zebrafish genetic stocks and research materials, 2) to provide consultation and pathology services, and 3) to develop a platform, based on a panel of PCR assays, to screen for the most prevalent pathogens of laboratory zebrafish. These materials, services and information will be made widely available to the research community. Diversity Supplement Summary/Abstract We apply for a diversity supplement to the P40 grant (OD011021) of the Zebrafish International Resource Center (ZIRC) for Mr. Corbin Jaid Schuster, an American Citizen and member of the Yakama Nation. The research plan ties Aims 2 and 3 of the ZIRC P40 grant to Corbin’s long-term career goal to employ the zebrafish model in research and to develop similar wild and aquaculture fish diagnostic screening services for the Yakama Nation. In turn, ZIRC will benefit from adopting the environmental sampling method and dPCR technology, which Corbin developed during his Ph.D.