Zebrafish International Resource Center

项目概要/摘要 斑马鱼是研究脊椎动物发育、生理、行为、遗传和 疾病强大的技术可以有效地产生和恢复斑马鱼突变， 调节发育模式、器官形成、生理和行为的基因。最近 这些进展使研究转基因斑马鱼中的基因功能变得容易， 寡核苷酸这些基因的功能在脊椎动物中是保守的， 组因此，对斑马鱼突变的分析提供了对其他脊椎动物基因功能的深入了解， 包括人类 斑马鱼国际资源中心已经建立，作为一个储存库， 动物，材料和服务的研究社区。本建议寻求继续供资1） 作为斑马鱼遗传资源和研究材料的中央储存库，2）提供 咨询和病理学服务，以及3）开发一个平台，基于一组PCR检测， 筛选实验室斑马鱼最常见的病原体。这些材料、服务和 将向研究界广泛提供资料。 多样性补充摘要/摘要 我们申请斑马鱼国际资源P40补助金（OD 011021）的多样性补充 中心（ZIRC）为美国公民和Yakama民族成员Corbin Jaid Schuster先生提供服务。的 研究计划将ZIRC P40赠款的目标2和3与科尔宾的长期职业目标联系起来， 研究斑马鱼模型，并开发类似的野生和水产养殖鱼类诊断筛选服务 为亚卡玛国效力反过来，ZIRC将受益于采用环境取样方法， dPCR技术，Corbin在他的博士学位期间开发。

Overall Project Summary/Abstract Zebrafish is a premiere organism to study vertebrate development, physiology, behavior, genetics and disease. Powerful techniques allow efficient generation and recovery of zebrafish mutations affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Recent advances make it easy to study gene function in transgenic zebrafish and with antisense oligonucleotides. The functions of many, if not most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene function in other vertebrates, including humans. The Zebrafish International Resource Center has been established as a repository that provides animals, materials and services to the research community. This proposal seeks continued funding 1) to serve as a central repository for zebrafish genetic stocks and research materials, 2) to provide consultation and pathology services, and 3) to develop a platform, based on a panel of PCR assays, to screen for the most prevalent pathogens of laboratory zebrafish. These materials, services and information will be made widely available to the research community. Diversity Supplement Summary/Abstract We apply for a diversity supplement to the P40 grant (OD011021) of the Zebrafish International Resource Center (ZIRC) for Mr. Corbin Jaid Schuster, an American Citizen and member of the Yakama Nation. The research plan ties Aims 2 and 3 of the ZIRC P40 grant to Corbin’s long-term career goal to employ the zebrafish model in research and to develop similar wild and aquaculture fish diagnostic screening services for the Yakama Nation. In turn, ZIRC will benefit from adopting the environmental sampling method and dPCR technology, which Corbin developed during his Ph.D.