权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Optimal Tissue Engineering Approach for Treating Injured Vocal Folds

治疗受损声带的最佳组织工程方法

基本信息

批准号：
10530598
负责人：
Donald O Freytes
金额：
$ 57.03万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-12-01 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10530598
关键词：
Address American Anatomy Architecture Area Autologous Biocompatible Materials Biomechanics Bladder Cell Therapy Cells Cellular Structures Cicatrix Clinical Clinical Trials Communication impairment Data Disease Dysphonia Engineering Etiology Extracellular Matrix Fibroblasts Fibrosis Foundations Gel Human Hydrogels Impairment In Vitro Inflammation Inflammatory Injectable Injury Investigation Laboratories Lamina Propria Location Macrophage Mechanics Mesenchymal Stem Cells Methods Modality Mucous Membrane National Institute on Deafness and Other Communication Disorders Organ Outcome Outpatients Particulate Patient-Focused Outcomes Patients Persons Physiology Pliability Pre-Clinical Model Predisposition Procedures Process Production Public Health Reflux Regenerative response Reporting Role Site Small Intestinal Submucosa Source Stimulus Stomach Strategic Planning Structure Symptoms Therapeutic Therapeutic Effect Time Tissue Engineering Tissues Trauma Treatment Cost Voice Voice Disorders Wages Work biomaterial development clinical application clinical implementation clinical material clinical practice delivery vehicle experimental study functional restoration healing iatrogenic injury implantation improved in vitro activity in vivo individual variation injured insight minimally invasive novel regenerative repaired response response to injury scaffold stem cells vocal cord wound healing

项目摘要

PROJECT SUMMARY Voice impairment is the most common communication disorder with nearly 20 million people in the US reporting symptoms of dysphonia annually. The cost of treatment and lost wages for this disorder is approaching $13 billion. The etiology of these disorders is diverse, but given their anatomic location, the vocal folds (VFs) are susceptible to a multitude of injurious stimuli, including reflux of gastric contents, iatrogenic injury, and the inherent trauma associated with voice production. Injury can result in altered lamina propria (LP) architecture resulting in aberrant phonatory physiology. To date, no treatment restores the native VF extracellular matrix (ECM) composition, structure, and function following VF injury which likely underlies suboptimal outcomes for patients with VF scar. ECMs, if appropriately processed, can be used as resorbable, clinically safe, and naturally derived biomaterials that promote tissue remodeling while reducing fibrosis. Injectable ECM hydrogels are ideal for clinical application and cell delivery, particularly given the emerging practice and distinct advantages of in-office procedures using minimally invasive approaches. Work from our group and others provide promising data regarding the role of decellularized vocal fold lamina propria (VFLP-ECM) as an injectable agent or as a delivery vehicle for therapeutic cells such as mesenchymal stem cells. We broadly hypothesize that VFLP-ECM hydrogels (with or without stem cells) will promote functional repair of the VFs.

项目概要声音障碍是最常见的沟通障碍，美国有近 2000 万人报告有声音障碍每年都会出现发声困难的症状。这种疾病的治疗费用和工资损失接近 13 美元十亿。这些疾病的病因多种多样，但鉴于其解剖位置，声带 (VF) 容易受到多种伤害性刺激，包括胃内容物反流、医源性损伤和与发声相关的固有创伤。损伤可能导致固有层 (LP) 结构改变导致发声生理异常。迄今为止，尚无任何治疗方法可以恢复天然心室颤动细胞外基质 VF 损伤后的 (ECM) 组成、结构和功能可能是导致结果不佳的基础有 VF 疤痕的患者。 ECM 如果经过适当处理，可用作可吸收的、临床安全的、天然衍生的生物材料促进组织重塑，同时减少纤维化。可注射 ECM 水凝胶是临床应用的理想选择和细胞递送，特别是考虑到使用办公室内程序的新兴实践和独特优势微创方法。我们小组和其他人的工作提供了有关作用的有希望的数据脱细胞声带固有层（VFLP-ECM）作为注射剂或作为递送载体治疗细胞，例如间充质干细胞。我们广泛假设 VFLP-ECM 水凝胶（带有或没有干细胞）将促进 VF 的功能修复。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro.

DOI：
10.1021/acsbiomaterials.9b01837
发表时间：
2020-03-09
期刊：
ACS biomaterials science & engineering
影响因子：
5.8
作者：
Mora-Navarro C;Badileanu A;Gracioso Martins AM;Ozpinar EW;Gaffney L;Huntress I;Harrell E;Enders JR;Peng X;Branski RC;Freytes DO
通讯作者：
Freytes DO

Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials.

DOI：
10.1039/d2bm01012g
发表时间：
2023-01-17
期刊：
BIOMATERIALS SCIENCE
影响因子：
6.6
作者：
Biehl, Andreea;Martins, Ana M. Gracioso M.;Davis, Zachary G. G.;Sze, Daphne;Collins, Leonard;Mora-Navarro, Camilo;Fisher, Matthew B. B.;Freytes, Donald O. O.
通讯作者：
Freytes, Donald O. O.

Mast Cell-Biomaterial Interactions and Tissue Repair.

DOI：
10.1089/ten.teb.2020.0275
发表时间：
2020-11
期刊：
Tissue engineering. Part B, Reviews
影响因子：
0
作者：
E. Ozpinar;Ariana L. Frey;G. Cruse;D. Freytes
通讯作者：
E. Ozpinar;Ariana L. Frey;G. Cruse;D. Freytes

Fast Automated Approach for the Derivation of Acellular Extracellular Matrix Scaffolds from Porcine Soft Tissues.

DOI：
10.1021/acsbiomaterials.0c00265
发表时间：
2020-07-13
期刊：
ACS biomaterials science & engineering
影响因子：
5.8
作者：
Badileanu A;Mora-Navarro C;Gracioso Martins AM;Garcia ME;Sze D;Ozpinar EW;Gaffney L;Enders JR;Branski RC;Freytes DO
通讯作者：
Freytes DO

Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds.