Single-Cell Epigenomics, Transcriptomics, and Bioinformatics Core

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SUMMARY The PCEN at UVA will support a combined biomedical research core to assist investigators in two important areas: 1) Transcriptome and epigenome technologies and 2) Bioinformatics. The Single Cell Epigenomics, Transcriptomics, and Bioinformatics core will be directed by experienced researchers (Silvia Medrano, Ph.D. and Nathan Sheffield, Ph.D.) and staffed with highly trained personnel. Through the Single Cell Epigenomics and Transcriptomics component of the core, the PCEN will generate new data that will require substantial single cell and bioinformatics resources and expertise. The Bioinformatics component of the core will work closely with the single cell component and provide both computational infrastructure and analytical support to ensure that the investigators convert this important data into knowledge. By integrating all these services within a single core, we will ensure that PCEN investigators carry out their research in a coordinated, cost-effective, and efficient manner. The core will serve as a resource for the two PCEN main Research Projects and Pilot Projects at all times. Furthermore, the core will support the broader pediatric kidney research community at UVA and other institutions. In addition to offering a variety of specialized, innovative and essential research services, techniques, and instrumentation, the core will provide hands-on training to students, postdocs, fellows and young scientists as well as consultation services and access to state-of-the-art research equipment. Moreover, the core will provide support for preparation of manuscripts and grant proposals. The scope of the core is well in line with the overall theme of this PCEN that is “Regulation of cell fate during kidney development and disease”. The experimental approaches of the PCEN involve examination of epigenetic and transcriptional mechanisms that control cell fate, plasticity, and identity and the cellular signals underlying the phenotypic transformation that occurs during kidney injury and repair. The proposed core will provide the specialized technologies and expertise needed to accomplish the stated goals of the PCEN.