ShEEP Request for High Resolution Desktop MicroCT System

SheEEP 请求高分辨率桌面 MicroCT 系统

• 批准号: 10538047
• 负责人: Joshua F. Yarrow
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10538047
• 关键词: 10 year old; 3-Dimensional; 3D Print; Address; Aging; Anatomy; Animal Model; Apatites; Award; Blood Vessels; Bone Density; Calcium; Chronic; Computer Workstations; Computer software; Core Facility; Cost Savings; Custom; Data; Data Set; Degenerative polyarthritis; Devices; Ensure; Equipment; Fascicle; Fees; Florida; Foundations; Funding; Future; Grant; Health; Health Benefit; Health system; Healthcare Systems; Hour; Image; Institution; Letters; Longevity; Maintenance; Malignant Neoplasms; Memory; Minor; Modeling; Morphology; Mus; Nerve; Nervous System Trauma; Obesity; Operating System; Osteoporosis; Phase; Positioning Attribute; Privatization; Procedures; Protocols documentation; Rattus; Research; Research Personnel; Resolution; Roentgen Rays; Rotation; Running; Sampling; Scanning; Schedule; Scientist; Services; Sheep; Source; Spinal cord injury; Spottings; Stains; Structure; Supervision; Surface; System; Technical Expertise; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Training; Tube; United States National Institutes of Health; Veterans; Wages; Work; Yarrow; base; bone; career development; contrast enhanced; cooperative study; cost; detector; end of life; high resolution imaging; imaging capabilities; improved; microCT; musculoskeletal imaging; operation; pre-clinical; preclinical imaging; programs; radiological imaging; reconstruction; recruit; rehabilitation research; response; soft tissue; submicron; success; three-dimensional visualization; time use; tomography

Requested Equipment: This request is for a Bruker Skyscan 1272 CMOS Edition high-resolution desktop microcomputed tomography (microCT) system, a 16-sample automatic tube changer with custom 3D printed sample tubes, a calcium-hydroxy apatite bone mineral density (BMD) phantom set, and a computer workstation with Bruker software necessary to operate the system and visualize / analyze 3D datasets. Equipment Justification: The North Florida / South Georgia Veterans Health System (NF/SGVHS) is the largest VA healthcare system in the US and has a robust research program with diverse imaging needs. To address this, NF/SGVHS established the Preclinical Musculoskeletal Imaging Core (PMIC) Lab, a centralized preclinical imaging core facility. For more than a decade, the PMIC Lab has provided access to and training on microCT systems, along with fee basis imaging, to scientists at NF/SGVHS and at other VA and academic centers. The Skyscan 1172 (old model) microCT is the most utilized PMIC Lab Core Equipment. However, this system is >10-years old and at its expected end-of-life. To facilitate our ongoing imaging needs, we request a Skyscan 1272 (new model) high-resolution microCT. The Skyscan 1272 is the highest resolution microCT on the market and images fine details as small as 0.45 µm (submicron resolution), using phase-contrast enhancement, which will improve our ability to image small anatomical structures (e.g., microvasculature, bone lacunae, and nerve fascicles). The 16-sample tube changer is an accessory that is exclusive to the Skyscan 1272, which automatically changes sample tubes upon completion of a scan and initiates each new scan via user programmed software. In doing so, the tube changer increases device availability by allowing continuous 24-hr/day microCT scanning without direct user supervision. This will permit us to grow our user base by simultaneously increasing device availability and by reducing microCT costs, due to less technician time. In doing so, we will be able to recruit Users who may not be able to typically afford high-resolution imaging (e.g., those collecting preliminary data for proposals), which will increase our likelihood of success on future VA and non-VA grants that are increasingly dependent upon the use of high-resolution imaging. Projected Use: We have 4 Major VA Users, 5 Minor VA Users, and 8 Academic Users (17 total users) who have ongoing or expected microCT needs. Major Users have VA Merit, Career Development, Cooperative Study, and/or Small Projects in Rehabilitation Research awards. Minor Users have funding via the same VA mechanisms and/or from NIH, DOD, or private foundations. Academic Users have tertiary microCT priority, which is facilitated via fee basis imaging, assuming the research does not interfere with Major / Minor Users and is relevant to Veterans’ health. We anticipate 40+ hours/week of microCT use throughout the lifespan of this equipment, based on past microCT use and future projections. VA Users will exceed 75% of machine time, with Major VA Users constituting >50% of use time. Projects Supported and Benefit to Veterans: The PMIC Lab has established numerous microCT protocols to assess the 3D microstructure of bone, vasculature, and other soft tissues. The Users on this proposal assess changes in these tissues in small animal models of spinal cord injury, osteoporosis, osteoarthritis, obesity, cancer, and other conditions that impact Veterans’ health. The requested Skyscan 1272 microCT system will allow the PMIC Lab to support the imaging needs of these investigators, which will ultimately benefit the health of Veterans, and will permit us to expand our microCT expertise to include the assessment of bone lacunae, microvasculature, and nerve fascicles that are best visualized at submicron resolutions.

请求的设备：此请求适用于 Bruker Skyscan 1272 CMOS 版高分辨率台式机 微计算机断层扫描 (microCT) 系统，具有定制 3D 打印功能的 16 个样本自动换管器 样品管、钙羟基磷灰石骨矿物质密度 (BMD) 模型组和计算机 工作站配备操作系统和可视化/分析 3D 数据集所需的布鲁克软件。 设备理由：北佛罗里达州/南乔治亚州退伍军人健康系统 (NF/SGVHS) 是 美国最大的 VA 医疗保健系统，拥有强大的研究计划，可满足不同的成像需求。到 为了解决这个问题，NF/SGVHS 建立了临床前肌肉骨骼成像核心 (PMIC) 实验室，这是一个集中化的实验室 临床前成像核心设施。十多年来，PMIC 实验室提供了以下方面的访问和培训： microCT 系统以及收费成像，为 NF/SGVHS 以及其他 VA 和学术机构的科学家提供 中心。 Skyscan 1172（旧型号）microCT 是最常用的 PMIC 实验室核心设备。然而，这 系统已使用超过 10 年并已达到预期使用寿命。为了满足我们持续的成像需求，我们要求 Skyscan 1272（新型号）高分辨率显微 CT。 Skyscan 1272 是目前分辨率最高的 microCT 市场和图像精细细节小至 0.45 µm（亚微米分辨率），使用相差 增强，这将提高我们对小解剖结构（例如微脉管系统、骨 腔隙和神经束）。 16 个样品试管更换器是 Skyscan 独有的配件 1272，扫描完成后自动更换样品管，并通过以下方式启动每次新扫描 用户编程的软件。在此过程中，换管器通过允许连续的 每天 24 小时 microCT 扫描，无需用户直接监督。这将使我们能够通过以下方式扩大我们的用户群 由于减少了技术人员的时间，同时提高了设备​​可用性并降低了 microCT 成本。在 这样做，我们将能够招募通常无法负担高分辨率成像的用户（例如， 那些为提案收集初步数据的人），这将增加我们在未来 VA 和 非 VA 拨款越来越依赖于高分辨率成像的使用。 预计用途：我们有 4 个主要 VA 用户、5 个次要 VA 用户和 8 个学术用户（总共 17 个用户） 有持续或预期的 microCT 需求。主要用户有VA优点，职业发展，合作 康复研究奖的研究和/或小型项目。未成年用户通过同一 VA 获得资金 机制和/或来自 NIH、DOD 或私人基金会。学术用户拥有第三级 microCT 优先权， 假设研究不会干扰主要/次要用户，这是通过收费成像来促进的 并且与退伍军人的健康有关。我们预计在整个生命周期中每周使用 microCT 的时间超过 40 小时 该设备基于过去的 microCT 使用和未来预测。 VA 用户将超过 75% 的机器时间， 主要 VA 用户占使用时间的 50% 以上。 支持的项目和对退伍军人的好处：PMIC 实验室已建立了许多 microCT 协议 评估骨骼、脉管系统和其他软组织的 3D 微观结构。此提案的用户 评估脊髓损伤、骨质疏松症、骨关节炎的小动物模型中这些组织的变化， 肥胖、癌症和其他影响退伍军人健康的疾病。请求的 Skyscan 1272 microCT 系统将使 PMIC 实验室能够支持这些研究人员的成像需求，最终将 有利于退伍军人的健康，并使我们能够扩展我们的 microCT 专业知识，以包括评估 骨腔、微脉管系统和神经束在亚微米分辨率下可视化效果最佳。