Immune signal perception and integration by cell surface receptors and peptide ligands

细胞表面受体和肽配体的免疫信号感知和整合

• 批准号: 10542334
• 负责人: Libo Shan
• 金额: --
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2023-08-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10542334
• 关键词: Animals; BAK1 gene; Cell Surface Receptors; Cell membrane; Cell surface; Cells; Complex; Cytoplasm; Eukaryota; Event; Feedback; Gene Expression; Goals; Growth; Homeostasis; Immune; Immune response; Immune signaling; Immunity; Infection; Interdisciplinary Study; Ligands; Mediating; Microbe; Modification; Molecular; Natural Immunity; Pathway interactions; Pattern; Pattern recognition receptor; Peptides; Perception; Phosphorylation; Phosphotransferases; Plants; Post-Translational Protein Processing; Postdoctoral Fellow; Process; Receptor Signaling; Reproduction; Research; Research Personnel; Resolution; Signal Pathway; Signal Transduction; Specificity; Ubiquitination; attenuation; environmental adaptation; fortification; graduate student; immunoregulation; microbial; novel; pathogen; receptor; spatiotemporal; training opportunity; undergraduate student

Immune signal perception and integration by cell surface receptors and peptide ligands Plants and animals rely on pattern-recognition receptors (PRRs) to detect infection by recognizing microbe- associated molecular patterns (MAMPs). Plant plasma membrane (PM)-resident receptor kinases (RKs) function as PRRs that sense MAMPs from diverse microbes and collectively contribute to plant immunity. Plant RKs also perceive a wide range of extrinsic and intrinsic signals and regulate growth, reproduction, and environmental adaptation. The signaling pathways mediated by many RKs converge at a subfamily of RKs, namely BAK1/SERKs, that function as shared coreceptors associating with various receptors upon ligand perception modulating plant immunity, growth, and immune homeostasis. The PM-tethered cytoplasmic kinase BIK1 associates with multiple PRR-BAK1 complexes and relays the intracellular signaling events. Microbial infection also induces the gene expression of many secreted peptides, which could function as immunomodulatory phytocytokines perceived by PRRs and fortify plant immunity. This proposal's long-term goal is to elucidate the mechanisms underlying pathogen signal perception and integration by PRR complexes via shared modules in coordination with phytocytokines for a rapid, specific, and robust immune response. The proposed research will address several key questions in the field. The project will elucidate how BIK1-associated PRR receptorsome dynamics are spatiotemporally regulated and relay the diverse immune signaling. As a critical kinase, BIK1 homeostasis, activities, and subcellular dynamics are regulated by the intertwined phosphorylation and ubiquitination modifications. The project will define how the shared BAK1/SERK coreceptors maintain the functional specificity in different receptorsomes. The studies will address how signaling specificity is regulated with the single-cell resolution at the organismal level. The project will also study how immunomodulatory phytocytokines coordinate with microbial patterns to mount effective immunity. The proposed interdisciplinary research will provide ample training opportunities for researchers at different levels, including underrepresented undergraduate and graduate students and postdoctoral fellows, and will advance our understanding of innate immunity and signal transduction at the whole organismal level by identifying new components and delineating novel pathways that regulate immune signal integration, signaling activation, attenuation, and specificity.

细胞表面受体和多肽配体对免疫信号的感知和整合 动植物依靠模式识别受体(PRRs)识别微生物，从而检测感染。 相关分子模式(MAMP)。植物质膜驻留受体蛋白(RKS)功能 作为PRR，从不同的微生物中感知MAMP，并共同促进植物免疫。工厂RKS也 感知广泛的外部和内部信号，并调节生长、繁殖和环境 适应。许多RK介导的信号通路汇聚在RK的一个亚家族，即 BAK1/SERKS，在配体感知时作为与各种受体相关的共享辅助受体发挥作用 调节植物免疫、生长和免疫动态平衡。PM-拴系胞浆蛋白激酶BIK1 与多个PRR-BAK1复合体结合，继而传递细胞内信号事件。微生物感染 还可诱导多种分泌肽的基因表达，这些多肽具有免疫调节作用。 PRRs感知的植物细胞因子，增强植物免疫力。这项提案的长期目标是澄清 PRR复合体通过共享模块感知和整合病原菌信号的机制 与植物细胞因子协调，以实现快速、特异和强大的免疫反应。拟议的研究将 解决该领域的几个关键问题。该项目将阐明BIK1相关的PRR受体是如何 动力学是时空调节的，并传递不同的免疫信号。作为一个关键的激酶，BIK1 动态平衡、活性和亚细胞动力学由相互交织的磷酸化和 泛素化修饰。该项目将定义共享的BAK1/Serk辅受体如何维持 不同受体的功能特异性。这些研究将解决信号特异性是如何被调节的 在生物体水平上使用单细胞分辨率。该项目还将研究免疫调节如何 植物细胞因子与微生物模式相协调，以建立有效的免疫。拟议的跨学科 研究将为不同级别的研究人员提供充足的培训机会，包括任职人数不足的人员。 本科生、研究生和博士后研究员，并将促进我们对先天的理解 通过识别新的成分和描绘整个生物体水平的免疫和信号转导 调节免疫信号整合、信号激活、衰减和特异性的新途径。