Immune signal perception and integration by cell surface receptors and peptide ligands

细胞表面受体和肽配体的免疫信号感知和整合

• 批准号: 10542334
• 负责人: Libo Shan
• 金额: --
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2023-08-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10542334
• 关键词: Animals; BAK1 gene; Cell Surface Receptors; Cell membrane; Cell surface; Cells; Complex; Cytoplasm; Eukaryota; Event; Feedback; Gene Expression; Goals; Growth; Homeostasis; Immune; Immune response; Immune signaling; Immunity; Infection; Interdisciplinary Study; Ligands; Mediating; Microbe; Modification; Molecular; Natural Immunity; Pathway interactions; Pattern; Pattern recognition receptor; Peptides; Perception; Phosphorylation; Phosphotransferases; Plants; Post-Translational Protein Processing; Postdoctoral Fellow; Process; Receptor Signaling; Reproduction; Research; Research Personnel; Resolution; Signal Pathway; Signal Transduction; Specificity; Ubiquitination; attenuation; environmental adaptation; fortification; graduate student; immunoregulation; microbial; novel; pathogen; receptor; spatiotemporal; training opportunity; undergraduate student

Immune signal perception and integration by cell surface receptors and peptide ligands Plants and animals rely on pattern-recognition receptors (PRRs) to detect infection by recognizing microbe- associated molecular patterns (MAMPs). Plant plasma membrane (PM)-resident receptor kinases (RKs) function as PRRs that sense MAMPs from diverse microbes and collectively contribute to plant immunity. Plant RKs also perceive a wide range of extrinsic and intrinsic signals and regulate growth, reproduction, and environmental adaptation. The signaling pathways mediated by many RKs converge at a subfamily of RKs, namely BAK1/SERKs, that function as shared coreceptors associating with various receptors upon ligand perception modulating plant immunity, growth, and immune homeostasis. The PM-tethered cytoplasmic kinase BIK1 associates with multiple PRR-BAK1 complexes and relays the intracellular signaling events. Microbial infection also induces the gene expression of many secreted peptides, which could function as immunomodulatory phytocytokines perceived by PRRs and fortify plant immunity. This proposal's long-term goal is to elucidate the mechanisms underlying pathogen signal perception and integration by PRR complexes via shared modules in coordination with phytocytokines for a rapid, specific, and robust immune response. The proposed research will address several key questions in the field. The project will elucidate how BIK1-associated PRR receptorsome dynamics are spatiotemporally regulated and relay the diverse immune signaling. As a critical kinase, BIK1 homeostasis, activities, and subcellular dynamics are regulated by the intertwined phosphorylation and ubiquitination modifications. The project will define how the shared BAK1/SERK coreceptors maintain the functional specificity in different receptorsomes. The studies will address how signaling specificity is regulated with the single-cell resolution at the organismal level. The project will also study how immunomodulatory phytocytokines coordinate with microbial patterns to mount effective immunity. The proposed interdisciplinary research will provide ample training opportunities for researchers at different levels, including underrepresented undergraduate and graduate students and postdoctoral fellows, and will advance our understanding of innate immunity and signal transduction at the whole organismal level by identifying new components and delineating novel pathways that regulate immune signal integration, signaling activation, attenuation, and specificity.

细胞表面受体和肽配体的免疫信号感知和整合