Yale Macaque Brain Resource

项目摘要 由于其与人类的亲缘关系密切，非人灵长类动物（NHP）的大脑持有 在理解人脑的结构、功能、发育和病理学方面具有巨大的潜力。 然而，许多障碍阻碍了NHP的研究（其中，重要的后勤，伦理和财务 负担）。耶鲁大学神经科学系收藏了一批独特的脑组织， NHP猕猴，年龄从胚胎到成年，在近五十年的时间里产生， Pasko Rakic博士和已故的Patricia Goldman-Rakic博士的实验室。该集合包括幻灯片集 用：（1）[3 H]胸腺嘧啶标记的连续切割的大脑，用于细胞出生日期测定;（2）用氚化氨基标记的大脑 用于神经束示踪的酸;（3）摘除和选择性皮质病变的猴脑;（4） 出生前X射线照射和超声暴露的猴，出生后处死;（5）EM块，从多个 胎儿和出生后的猴子的大脑区域;以及，在去年增加了，（6）组织化学和生物化学 处理的脑组织和（7）未处理的NHP脑组织块。 虽然MBR的过度具体目标保持不变，但已进行了适当的调整，有利于 支持正在进行的研究项目，并扩大MBR的范围，以适应新的。此续订 建议：1）继续对MBR集合1至4进行清点、编目、恢复和添加材料。2)到 组织化学和免疫化学处理组织以扩大收集6并添加未处理组织块 至收藏7。3)继续宣传和传播NHP大脑信息。4)继续促进 资源的使用。当前更新申请的主要目的是维持MBR的运行， 通过为不同神经元和非神经元标记物的组织处理提供资金来促进其生长（在M & 7个发育不同年龄组的F标本）添加到第6批标本中。此外，脑组织 对于不同处理可行的块将继续被添加到集合7中。这项资助的宏伟目标 保持不变，使这些独特的收藏成为随时可用的国际研究资源 对神经科学界的影响如果获得资助，MBR将继续允许和促进NHP大脑研究 而不需要额外的动物牺牲。这避免了昂贵的重复实验和不必要的 使用动物。

PROJECT SUMMARY Because of its close phylogenetic relationship to the human, the non-human primate (NHP) brain holds enormous potential for understanding structure, function, development and pathology of the human brain. However, many obstacles impede studies of NHPs (among these, significant logistic, ethical, and financial burdens). The Department of Neuroscience at Yale University houses a unique collection of brain tissue from NHP macaques, ranging in age from embryonic to adult, which was generated over almost five decades in the laboratories of Dr. Pasko Rakic and the late Dr. Patricia Goldman-Rakic. The collection includes sets of slides with: (1) [3H]thymidine-labeled serially cut brains for cell birth dating; (2) brains labeled with tritiated amino acids for tract tracing; (3) brains from monkeys with enucleations and selected cortical lesions; (4) brains from prenatally X-irradiated and ultrasound-exposed monkeys sacrificed postnatally; (5) EM blocks from multiple brain regions of fetal and postnatal monkeys; and, added in the last year, (6) histo- and immunohistochemically processed brain tissue and (7) unprocessed NHP brain tissue blocks. While MBR’s overreaching Specific Aims remain the same, proper adjustments have been made, conducive to support ongoing research projects and to grow MBR in scope to accommodate new ones. This renewal proposes: 1) To continue to inventory, catalogue, restore and add materials to MBR Collections 1 to 4. 2) To histo- and immunohistochemically process tissue to expand collection 6 and to add unprocessed tissue blocks to collection 7. 3) To continue to advertise and disseminate NHP brain information. 4) To continue to facilitate use of the resource. A main purpose of the current renewal application is maintain MBR’s operations and to facilitate its growth by funding the processing of tissue for different neuronal and non-neuronal markers (in M & F specimens of 7 developmentally distinct age groups) to be added to collection 6. In addition, brain tissue blocks viable for different treatments will continue to be added to collection 7. The grand objective of this grant remains the same, to make these unique collections an international research resource that is readily available to the neuroscience community at-large. If funded, MBR will continue to permit and foster NHP brain research without requiring additional animal sacrifice. This avoids costly duplication of experiments and unnecessary use of animals.