Role of MIF and CD74 in the pathogenesis of emphysema

MIF和CD74在肺气肿发病机制中的作用

• 批准号: 10543511
• 负责人: Maor Sauler
• 金额: $ 68.48万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10543511
• 关键词: Address; Agonist; Alleles; Apoptosis; Attenuated; Biology; Cause of Death; Cell Aging; Cells; Chronic; Chronic Obstructive Pulmonary Disease; Cigarette smoke-induced emphysema; Cytoprotection; DNA Damage; Data; Development; Disease; Disease Progression; Endothelial Cells; Endothelium; Flow Cytometry; Genetic; Genetic Polymorphism; Genetic study; Goals; Histology; Homeostasis; Human; Image; Immune; LoxP-flanked allele; Lung; Lung diseases; MAPK3 gene; Macrophage; Mediating; Methods; Migration Inhibitory Factor; Molecular; Mouse Strains; Mus; Oxidative Stress; Oxidative Stress Induction; Pathogenesis; Pathologic; Pathway interactions; Patients; Predisposition; Proteins; Pulmonary Emphysema; Reporter; Research Personnel; Risk Factors; Role; Severities; Severity of illness; Signal Pathway; Signal Transduction; Slice; Smoker; Study models; Testing; Therapeutic; Tissues; Translating; Treatment Efficacy; United States; Visualization; Work; X-Ray Computed Tomography; cell type; cigarette smoke; cigarette smoke-induced; cytokine; effective therapy; exposure to cigarette smoke; gain of function; improved; loss of function; migration; modifiable risk; mouse model; new therapeutic target; novel; oxidative DNA damage; pharmacologic; promoter; protective effect; receptor; resilience; response; senescence; small molecule; stress reduction

PROJECT SUMMARY Emphysema is a common pathologic manifestation of Chronic Obstructive Pulmonary Disease (COPD), the fourth leading cause of death in the United States. The most important modifiable risk factor for emphysema is chronic exposure to cigarette smoke (CS). Yet, not all smokers develop emphysema and, therefore, cellular responses to CS are important mechanisms underlying disease progression. Our long-term goal is to define the cellular responses to CS and oxidative stress that protect the lung from emphysema. We have identified a cytoprotective role for the innate immune cytokine Macrophage Migration Inhibitory (MIF). MIF decreases oxidative stress and consequences of oxidative stress (e.g. cellular senescence and apoptosis) in lung endothelial cells, and mice with a genetic deletion of Mif are susceptible to emphysema. MIF is secreted in response to CS, but, paradoxically, MIF is decreased in patients with severe COPD. Targeting MIF, or its downstream pathways, may be therapeutic. However, MIF is a pleiotropic molecule with broad regulatory effects. To translate MIF biology into therapy, it will be essential to dissect out its cytoprotective pathways. Our goal for this proposal is to determine the mechanisms through which MIF antagonizes the development of emphysema. Our preliminary data suggests MIF mediates its protective effects through its receptor CD74. Our hypothesis is MIF protects against the development of emphysema by mitigating CS-mediated endothelial senescence in a CD74 dependent manner. In Aim 1, we will define the mechanism through which the MIF-CD74 interaction reduces endothelial senescence. In Aim 2, we will determine the role of endothelial CD74 in regulating susceptibility to emphysema. In Aim 3, we will determine the therapeutic potential of targeting MIF-CD74 interactions in emphysema. Completion of these aims will significantly advance our understanding of the pathogenesis of emphysema and may identify precision-based approaches based on MIF biology for treating patients with emphysema.

项目摘要 肺气肿是慢性阻塞性肺病（COPD）的常见病理表现， 美国第四大死因肺气肿最重要的可改变风险因素是 长期暴露于香烟烟雾（CS）。然而，并非所有吸烟者都会患肺气肿，因此， 对CS的反应是疾病进展的重要机制。我们的长期目标是 对CS和氧化应激的细胞反应，保护肺免受肺气肿。我们已经确定了一 先天性免疫细胞因子巨噬细胞迁移抑制因子（MIF）的细胞保护作用。MIF降低 肺中的氧化应激和氧化应激的后果（例如细胞衰老和凋亡） 内皮细胞和Mif基因缺失的小鼠易患肺气肿。MIF分泌于 但是，矛盾的是，严重COPD患者的MIF降低。针对MIF，或其 下游通路，可能是治疗性的。然而，MIF是具有广泛调节作用的多效性分子。 为了将MIF生物学转化为治疗，必须剖析其细胞保护途径。我们的目标 该建议是为了确定MIF对抗肺气肿发展的机制。 我们的初步数据表明MIF通过其受体CD 74介导其保护作用。我们的假设是 MIF通过减轻CS介导的内皮细胞衰老来防止肺气肿的发展， CD 74依赖性。在目标1中，我们将定义MIF-CD 74相互作用的机制。 减少内皮衰老。在目标2中，我们将确定内皮细胞CD 74在调节细胞凋亡中的作用。 易患肺气肿在目标3中，我们将确定靶向MIF-CD 74的治疗潜力。 肺气肿中的相互作用。这些目标的实现将大大促进我们对 肺气肿的发病机制，并可以确定基于MIF生物学的精确方法，用于治疗 肺气肿患者。