Lipid Peroxidation Analytical Core

脂质过氧化分析核心

• 批准号: 10544053
• 负责人: SEAN Stephen DAVIES
• 金额: $ 24.65万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10544053
• 关键词: Animal Model; Animals; Atherosclerosis; Biological; Blood; Cell model; Chemicals; Chronic Kidney Failure; Cultured Cells; Disease; Ensure; F2-Isoprostanes; Familial Hypercholesterolemia; Functional disorder; Goals; Half-Life; High Density Lipoproteins; Human; Individual; Instruction; Investigation; Lipid Peroxidation; Lipids; Liquid substance; Lysine; Malondialdehyde; Measurement; Measures; Medicine; Metabolism; Modification; Participant; Phosphatidylethanolamine; Post-Translational Protein Processing; Proteins; Pyridoxamine; Reaction; Reagent; Research Personnel; Rheumatoid Arthritis; Role; Sampling; Testing; Tissue Sample; Tissues; Vitamin B6; adduct; analog; atherosclerosis risk; experimental study; human disease; in vivo; instrumentation; methyl group; programs; small molecule

CORE C PROJECT SUMMARY The overall goal of this program project is to test the contribution of changes in high density lipoprotein (HDL) function in human disease, specifically atherosclerosis. One of the major hypotheses tested is that the lipid peroxidation that occurs during various condition that increase the risk for atherosclerosis (e.g. familial hypercholesterolemia and chronic kidney disease) causes HDL to become modified by reactive lipid dicarbonyls and that these modifications markedly alter HDL function. This core will provide synthetic versions of these reactive dicarbonyls to project investigators so that they can test the effects of HDL modification. This core will also provide synthetic compounds that act as dicarbonyl scavengers and that can be used in both cultured cells, in animals, and in humans to block the reaction of dicarbonys with proteins and phosphatidylethanolamine. These scavengers are useful both to test the contribution of these dicarbonyls and as potential medicinal agents. Finally, this core will measure dicarbonyl adducts on proteins and phosphatidylethanolamine. Aim 1: Synthesize IsoLG and other dicarbonyls for testing in cultured cell and animal models. Aim 2: Synthesize dicarbonyl scavengers for testing in cultured cell and animal models. Aim 3: Measure the amounts of dicarbonyl modified proteins and phosphatidylethanolamines that are formed in tissue samples and fluids in animal models and humans with disease.

Core c项目总结