Actin Cytoskeleton Network Self-Organization
Actin Cytoskeleton Network Self-Organization
批准号:
10543764
负责人:
David R Kovar
金额:
$40.94万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2007
资助国家:
美国
项目状态:
已结题
起止时间:
2007-09-01 至 2024-12-31
关键词:
Actin-Binding ProteinActinsArchitectureBehaviorBehavioral MechanismsBindingBiochemicalBiologicalBiological AssayCellsCharacteristicsCollectionColorComplexCytoplasmCytoskeletonDevelopmentDiseaseEndocytosisExcisionF-ActinFeedbackFimbrinFission YeastFundingG ActinGoalsImageIn VitroIndividualInvestigationMediatingMicrofilamentsMolecularPeptide Initiation FactorsPlayProcessPropertyProtein IsoformsProtein SortingsProteinsRoleShapesSortingSurveysTestingTotal Internal Reflection FluorescentTropomyosinWorkalpha Actinincell assemblycell motilitycofilindensitygenetic regulatory proteinimaging approachmonomermutantprofilinreconstitutionself organizationsingle molecule
中文摘要
项目摘要/总结
英文摘要
PROJECT ABSTRACT/SUMMARY
Cells assemble functionally diverse actin cytoskeleton networks with distinct architectures and
dynamics to drive fundamental processes such as polarization, endocytosis, motility and division. The
specific characteristics of different actin filament networks (actin filament density, organization and
dynamics) are determined through the coordination action of specific sets of actin binding proteins
(ABPs) with complementary binding properties. Most investigations primarily focus on individual F-actin
networks. However, this provides limited overall understanding of F-actin network organization and
function because cells typically assemble and use multiple F-actin networks simultaneously within the
same cytoplasm. Consequently, F-actin networks must self-organize from a common pool of shared
actin monomers and overlapping sets of ABPs. We have predicted that there are important interactions
(cross talk) between networks that are critical for their form and function. Our long-term goal is to
discover the direct and indirect interactions between self-organized F-actin networks, which are critical
for establishing their unique identities and functions within a common cytoplasm, and to determine the
underlying molecular mechanistic principles that govern these interactions.
We are investigating two major actin cytoskeleton self-organization questions. The first is to
determine the mechanisms by which the size and density of F-actin networks are regulated by
competition for a limiting amount of actin monomers (Aim I). Although unassembled G-actin was not
thought to be limiting, we systematically showed that competition for G-actin helps control the size and
density of competitive F-actin networks in fission yeast, and that the actin monomer protein profilin plays
a major role in regulating competition for limiting G-actin. Our goal is to determine the underlying
mechanism by which profilin and other ABPs contribute to the proper distribution of G-actin between
functionally diverse actin cytoskeleton networks. The second is to determine how diverse F-actin
networks acquire the specific set of ABPs whose complementary biochemical activities help define their
form and function (Aim II). We will investigate the underlying intrinsic molecular mechanisms by which
ABPs self-sort to particular F-actin networks within a common cytoplasm, including (1) the contribution of
competition and cooperation between ABPs for associating with actin filaments, and (2) whether actin
assembly factors initiate self-sorting by biasing the association of particular ABPs.
期刊论文(37)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
When Is "Enough" Enough?
什么时候“够了”?
DOI:
10.1016/j.cels.2017.05.007
发表时间:
2017
期刊:
Cell systems
影响因子:
9.3
作者:
[Suarez,Cristian, McCall,PatrickM, Gardel,MargaretL, Kovar,DavidR]
通讯作者:
Kovar,DavidR
DOI:
10.1016/j.cub.2015.06.033
发表时间:
2015-08-03
期刊:
Current biology : CB
影响因子:
--
作者:
[Zimmermann D, Santos A, Kovar DR, Rock RS]
通讯作者:
Rock RS
DOI:
10.1038/ncb2109
发表时间:
2010-11
期刊:
Nature cell biology
影响因子:
21.3
作者:
[Haglund CM, Choe JE, Skau CT, Kovar DR, Welch MD]
通讯作者:
Welch MD
Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast.
DOI:
10.1016/j.cub.2010.06.020
发表时间:
2010-08-24
期刊:
CURRENT BIOLOGY
影响因子:
9.2
作者:
[Skau, Colleen T., Kovar, David R.]
通讯作者:
Kovar, David R.
Cytoskeletal dynamics: a view from the membrane.
细胞骨架动力学:膜的视图。
DOI:
10.1083/jcb.201502062
发表时间:
2015-05-11
期刊:
The Journal of cell biology
影响因子:
--
作者:
[Bezanilla M, Gladfelter AS, Kovar DR, Lee WL]
通讯作者:
Lee WL
共 18 条
Dynamic control of actin network architecture in early C. elegans embryos
-
批准号:10280989
-
项目类别:
-
资助金额:$47.34万
-
财政年份:2021
-
负责人:David R Kovar
-
依托单位:
Dynamic control of actin network architecture in early C. elegans embryos
-
批准号:10461861
-
项目类别:
-
资助金额:$47.34万
-
财政年份:2021
-
负责人:David R Kovar
-
依托单位:
Dynamic control of actin network architecture in early C. elegans embryos
-
批准号:10630246
-
项目类别:
-
资助金额:$47.35万
-
财政年份:2021
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
-
批准号:8136522
-
项目类别:
-
资助金额:$26.7万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly - Renewal 01 - Resubmission
-
批准号:8985681
-
项目类别:
-
资助金额:$38.66万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
-
批准号:7928718
-
项目类别:
-
资助金额:$26.97万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Actin Cytoskeleton Network Self-Organization
-
批准号:10319960
-
项目类别:
-
资助金额:$40.94万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
-
批准号:7318798
-
项目类别:
-
资助金额:$25.91万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
-
批准号:7482445
-
项目类别:
-
资助金额:$26.49万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Actin Cytoskeleton Network Self-Organization
-
批准号:9887915
-
项目类别:
-
资助金额:$43.88万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Actin Cytoskeleton Network Self-Organization
-
批准号:10083213
-
项目类别:
-
资助金额:$40.93万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly - Renewal 01 - Resubmission
-
批准号:8632044
-
项目类别:
-
资助金额:$38.66万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
-
批准号:7679411
-
项目类别:
-
资助金额:$27.26万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Mechanisms of Formin-Mediated Actin Filament Assembly - Renewal 01 - Resubmission
-
批准号:8788039
-
项目类别:
-
资助金额:$38.66万
-
财政年份:2007
-
负责人:David R Kovar
-
依托单位:
Analysis of Actomyosin Ring Assembly and Contraction
-
批准号:6551593
-
项目类别:
-
资助金额:$3.83万
-
财政年份:2002
-
负责人:David R Kovar
-
依托单位:
Analysis of Actomyosin Ring Assembly and Contraction
-
批准号:6758568
-
项目类别:
-
资助金额:$2.45万
-
财政年份:2002
-
负责人:David R Kovar
-
依托单位:
Analysis of Actomyosin Ring Assembly and Contraction
-
批准号:6603291
-
项目类别:
-
资助金额:$4.64万
-
财政年份:2002
-
负责人:David R Kovar
-
依托单位:
海外基金