Actin Cytoskeleton Network Self-Organization
肌动蛋白细胞骨架网络自组织
基本信息
- 批准号:10543764
- 负责人:
- 金额:$ 40.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-09-01 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:Actin-Binding ProteinActinsArchitectureBehaviorBehavioral MechanismsBindingBiochemicalBiologicalBiological AssayCellsCharacteristicsCollectionColorComplexCytoplasmCytoskeletonDevelopmentDiseaseEndocytosisExcisionF-ActinFeedbackFimbrinFission YeastFundingG ActinGoalsImageIn VitroIndividualInvestigationMediatingMicrofilamentsMolecularPeptide Initiation FactorsPlayProcessPropertyProtein IsoformsProtein SortingsProteinsRoleShapesSortingSurveysTestingTotal Internal Reflection FluorescentTropomyosinWorkalpha Actinincell assemblycell motilitycofilindensitygenetic regulatory proteinimaging approachmonomermutantprofilinreconstitutionself organizationsingle molecule
项目摘要
PROJECT ABSTRACT/SUMMARY
Cells assemble functionally diverse actin cytoskeleton networks with distinct architectures and
dynamics to drive fundamental processes such as polarization, endocytosis, motility and division. The
specific characteristics of different actin filament networks (actin filament density, organization and
dynamics) are determined through the coordination action of specific sets of actin binding proteins
(ABPs) with complementary binding properties. Most investigations primarily focus on individual F-actin
networks. However, this provides limited overall understanding of F-actin network organization and
function because cells typically assemble and use multiple F-actin networks simultaneously within the
same cytoplasm. Consequently, F-actin networks must self-organize from a common pool of shared
actin monomers and overlapping sets of ABPs. We have predicted that there are important interactions
(cross talk) between networks that are critical for their form and function. Our long-term goal is to
discover the direct and indirect interactions between self-organized F-actin networks, which are critical
for establishing their unique identities and functions within a common cytoplasm, and to determine the
underlying molecular mechanistic principles that govern these interactions.
We are investigating two major actin cytoskeleton self-organization questions. The first is to
determine the mechanisms by which the size and density of F-actin networks are regulated by
competition for a limiting amount of actin monomers (Aim I). Although unassembled G-actin was not
thought to be limiting, we systematically showed that competition for G-actin helps control the size and
density of competitive F-actin networks in fission yeast, and that the actin monomer protein profilin plays
a major role in regulating competition for limiting G-actin. Our goal is to determine the underlying
mechanism by which profilin and other ABPs contribute to the proper distribution of G-actin between
functionally diverse actin cytoskeleton networks. The second is to determine how diverse F-actin
networks acquire the specific set of ABPs whose complementary biochemical activities help define their
form and function (Aim II). We will investigate the underlying intrinsic molecular mechanisms by which
ABPs self-sort to particular F-actin networks within a common cytoplasm, including (1) the contribution of
competition and cooperation between ABPs for associating with actin filaments, and (2) whether actin
assembly factors initiate self-sorting by biasing the association of particular ABPs.
项目摘要/总结
细胞组装具有不同结构的功能多样的肌动蛋白细胞骨架网络,
动力学驱动基本过程,如极化,内吞作用,运动和分裂。的
不同肌动蛋白丝网络的具体特征(肌动蛋白丝密度,组织和
动力学)是通过特定的肌动蛋白结合蛋白的协调作用来确定的
(ABPs)具有互补结合特性。大多数研究主要集中在单个F-肌动蛋白
网络.然而,这提供了对F-肌动蛋白网络组织的有限的整体理解,
因为细胞通常在细胞内同时组装和使用多个F-肌动蛋白网络,
相同的细胞质。因此,F-肌动蛋白网络必须从一个共享的共同池中自组织起来。
肌动蛋白单体和ABP重叠组。我们已经预测到,
(串扰)网络之间的形式和功能至关重要。我们的长期目标是
发现自组织F-肌动蛋白网络之间的直接和间接相互作用,这是至关重要的
在一个共同的细胞质中建立它们独特的身份和功能,并确定它们的基因型。
控制这些相互作用的潜在分子机械原理。
我们正在研究两个主要的肌动蛋白细胞骨架自组织的问题。一是
确定F-肌动蛋白网络的大小和密度受以下因素调节的机制:
竞争有限数量的肌动蛋白单体(目的I)。虽然未组装的G-肌动蛋白
被认为是限制,我们系统地表明,竞争G-肌动蛋白有助于控制大小,
在分裂酵母中竞争性F-肌动蛋白网络的密度,以及肌动蛋白单体蛋白profilin发挥的作用,
在限制G-肌动蛋白的竞争中起主要作用。我们的目标是确定
profilin和其他ABPs有助于G-肌动蛋白在细胞间的适当分布的机制
功能多样的肌动蛋白细胞骨架网络。第二个是确定F-肌动蛋白
网络获得一组特定的ABP,其互补的生化活动有助于定义其功能。
形式和功能(目标二)。我们将研究潜在的内在分子机制,
ABPs在一个共同的细胞质内自我分选到特定的F-肌动蛋白网络,包括(1)
ABPs之间的竞争和合作与肌动蛋白丝,以及(2)是否肌动蛋白
组装因子通过偏置特定ABP的缔合来启动自分选。
项目成果
期刊论文数量(37)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
When Is "Enough" Enough?
什么时候“够了”?
- DOI:10.1016/j.cels.2017.05.007
- 发表时间:2017
- 期刊:
- 影响因子:9.3
- 作者:Suarez,Cristian;McCall,PatrickM;Gardel,MargaretL;Kovar,DavidR
- 通讯作者:Kovar,DavidR
Actin age orchestrates myosin-5 and myosin-6 run lengths.
- DOI:10.1016/j.cub.2015.06.033
- 发表时间:2015-08-03
- 期刊:
- 影响因子:0
- 作者:Zimmermann D;Santos A;Kovar DR;Rock RS
- 通讯作者:Rock RS
Rickettsia Sca2 is a bacterial formin-like mediator of actin-based motility.
- DOI:10.1038/ncb2109
- 发表时间:2010-11
- 期刊:
- 影响因子:21.3
- 作者:Haglund CM;Choe JE;Skau CT;Kovar DR;Welch MD
- 通讯作者:Welch MD
Fimbrin and tropomyosin competition regulates endocytosis and cytokinesis kinetics in fission yeast.
- DOI:10.1016/j.cub.2010.06.020
- 发表时间:2010-08-24
- 期刊:
- 影响因子:9.2
- 作者:Skau, Colleen T.;Kovar, David R.
- 通讯作者:Kovar, David R.
Cytoskeletal dynamics: a view from the membrane.
细胞骨架动力学:膜的视图。
- DOI:10.1083/jcb.201502062
- 发表时间:2015-05-11
- 期刊:
- 影响因子:0
- 作者:Bezanilla M;Gladfelter AS;Kovar DR;Lee WL
- 通讯作者:Lee WL
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
David R Kovar其他文献
David R Kovar的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('David R Kovar', 18)}}的其他基金
Dynamic control of actin network architecture in early C. elegans embryos
早期秀丽隐杆线虫胚胎中肌动蛋白网络结构的动态控制
- 批准号:
10461861 - 财政年份:2021
- 资助金额:
$ 40.94万 - 项目类别:
Dynamic control of actin network architecture in early C. elegans embryos
早期秀丽隐杆线虫胚胎中肌动蛋白网络结构的动态控制
- 批准号:
10280989 - 财政年份:2021
- 资助金额:
$ 40.94万 - 项目类别:
Dynamic control of actin network architecture in early C. elegans embryos
早期秀丽隐杆线虫胚胎中肌动蛋白网络结构的动态控制
- 批准号:
10630246 - 财政年份:2021
- 资助金额:
$ 40.94万 - 项目类别:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
裂殖酵母中福尔明介导的肌动蛋白丝组装机制
- 批准号:
8136522 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
Mechanisms of Formin-Mediated Actin Filament Assembly - Renewal 01 - Resubmission
福尔明介导的肌动蛋白丝组装机制 - 续订 01 - 重新提交
- 批准号:
8985681 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
裂殖酵母中福尔明介导的肌动蛋白丝组装机制
- 批准号:
7928718 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
裂殖酵母中福尔明介导的肌动蛋白丝组装机制
- 批准号:
7318798 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
Mechanisms of Formin-Mediated Actin Filament Assembly in Fission Yeast
裂殖酵母中福尔明介导的肌动蛋白丝组装机制
- 批准号:
7482445 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
相似海外基金
A novel motility system driven by two classes of bacterial actins MreB
由两类细菌肌动蛋白 MreB 驱动的新型运动系统
- 批准号:
22KJ2613 - 财政年份:2023
- 资助金额:
$ 40.94万 - 项目类别:
Grant-in-Aid for JSPS Fellows
The structural basis of plasmid segregation by bacterial actins
细菌肌动蛋白分离质粒的结构基础
- 批准号:
342887 - 财政年份:2016
- 资助金额:
$ 40.94万 - 项目类别:
Operating Grants
The structural basis for plasmid segregation by bacterial actins
细菌肌动蛋白分离质粒的结构基础
- 批准号:
278338 - 财政年份:2013
- 资助金额:
$ 40.94万 - 项目类别:
Operating Grants
Cytoplasmic Actins in Maintenance of Muscle Mitochondria
细胞质肌动蛋白在维持肌肉线粒体中的作用
- 批准号:
8505938 - 财政年份:2012
- 资助金额:
$ 40.94万 - 项目类别:
Differential Expression of the Diverse Plant Actins
多种植物肌动蛋白的差异表达
- 批准号:
7931495 - 财政年份:2009
- 资助金额:
$ 40.94万 - 项目类别:
Studies on how actins and microtubules are coordinated and its relevancy.
研究肌动蛋白和微管如何协调及其相关性。
- 批准号:
19390048 - 财政年份:2007
- 资助金额:
$ 40.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Interaction of myosin with monomeric actins
肌球蛋白与单体肌动蛋白的相互作用
- 批准号:
5311554 - 财政年份:2001
- 资助金额:
$ 40.94万 - 项目类别:
Priority Programmes
STRUCTURE/INTERACTIONS OF ACTINS AND ACTIN-BINDING PROTEIN
肌动蛋白和肌动蛋白结合蛋白的结构/相互作用
- 批准号:
6316669 - 财政年份:2000
- 资助金额:
$ 40.94万 - 项目类别: