RIBOZYMES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 像蛋白质一样，RNA折叠成功能所需的明确定义的三维结构。RNA折叠问题旨在理解RNA结构建立的分子和动力学原理。解决RNA折叠问题需要了解折叠中间体的结构。我们的长期目标是确定一系列平衡和动力学折叠中间体的结构，以充分描绘不同三级RNA的整个折叠途径。我们的短期目标是建立的cryo-EM技术，重量小于100 kDa的RNA的天然和折叠中间体的结构研究的可行性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Like proteins, RNA folds into well-defined three-dimensional structures required for function. The RNA folding problem aims to understand the molecular and dynamic principles upon which RNA structures are built. Solving the RNA folding problem requires knowledge of the structure of folding intermediates. Our long-term goal is to determine the structures of a series of equilibrium and kinetic folding intermediates to fully delineate an entire folding pathway for different tertiary RNAs. Our short term goal is to establish the feasibility of the cryo-EM technique for structure studies of native and folding intermediates of RNAs weighing less than 100 kDa.