Core B: Biospecimen Core

核心 B：生物样本核心

• 批准号: 10555894
• 负责人: Alifiya Kapasi
• 金额: $ 24.77万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-30 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10555894
• 关键词: 3-Dimensional; Affect; Aging; Alzheimer' s Disease; Alzheimer' s disease brain; Alzheimer' s disease pathology; Area; Atlases; Atrophic; Attention; Autopsy; Brain; Brain region; Cells; Cerebral Amyloid Angiopathy; Characteristics; Clinical; Collaborations; Collection; Communities; Computer Analysis; Consent; Data; Databases; Development; Diagnosis; Elderly; Ensure; Evaluation; Generations; Genetic; Goals; Human; Image; Impaired cognition; Indirect Immunofluorescence; International; Laboratories; Link; Longevity; Management Information Systems; Measurement; Measures; Methodology; Minority; Molecular; Monitor; Neocortex; Neurodegenerative Disorders; New York; Office of Administrative Management; Outcome Measure; Participant; Pathologic; Pathology; Phenotype; Positioning Attribute; Prefrontal Cortex; Preparation; Process; Production; Proteomics; Protocols documentation; Resources; Sampling; Services; Shipping; Site; Source; Specimen; Standardization; Structure; Superior temporal gyrus; Techniques; Testing; Tissue Banks; Tissues; Training Programs; Universities; age effect; aging brain; biobank; brain tissue; clinical phenotype; cohort; data sharing; experimental study; improved; innovation; interest; method development; multidimensional data; multiple omics; neocortical; pathological aging; phenotypic data; programs; prospective; research study; sample collection; therapeutic development; tissue processing; trait; transcriptomics

BIOSPECIMEN CORE: PROJECT SUMMARY/ABSTRACT The overarching goal of our “3-D Aging & Alzheimer Brain Program” is to define, using single cell-based approaches with spatially-resolved transcriptomic and proteomic measurements, the effect of aging and Alzheimer’s disease (AD)-related pathologies on the brain in three dimensions and the relation of these effects on cognitive decline. Crucial to achieving this goal is consistent access to well-phenotyped, high quality, human brain tissue that has been consented for broad data sharing. The Biospecimen (BIO) Core will be responsible for compiling one of the largest collections of tissue across the lifespan and known pathological hallmarks of AD by continuing prospective collection of comparably phenotyped high quality cases at the University of Edinburgh, the Rush Alzheimer’s Disease Center, and the New York Brain Bank (NYBB). The goal of the BIO Core is to link clinical-pathologic data with contemporary molecular techniques from brain tissue to facilitate innovative research studies on aging and AD. The BIO core will (1) support each of the four Projects by identifying, preparing, and distributing the best available samples for each aim, (2) determine the plane of section for each sample, and (3) assemble standardized outcome measures from each sample. The BIO Core will also support the innovative methodological development of the Spatial Multiomics (SM) Core through the acquisition and provision of high-quality molecular grade tissues. In parallel, the harmonized phenotypic data will flow to the Integrated Computational Analysis (ICA) Core which will align the phenotypes with data for each of the Projects. A Laboratory Information Management System (LIMS), managed by the Administrative Core, will be used to organize and track all sample characteristics, enabling a structured evaluation of the sample selection and preparation process to uncover any areas of weakness and identify new quality measures. In summary, the BIO Core will significantly enhance the quality, annotation, and re-purposability of the data produced by this Program and its processes and protocols will help guide and enhance improvements in tissue processing for the wider neurodegenerative disease community.

生物样本核心：项目总结/摘要 我们的“3-D衰老和阿尔茨海默病大脑计划”的首要目标是定义，使用基于单细胞的 空间分辨转录组学和蛋白质组学测量的方法，衰老的影响， 阿尔茨海默病（AD）相关的病理学在三维脑和这些影响的关系 认知能力下降实现这一目标的关键是始终如一地获得表型良好、高质量、人类 已经同意进行广泛数据共享的脑组织。生物样本（BIO）核心将负责 收集了AD患者一生中最大的组织样本和已知的病理特征 通过在爱丁堡大学继续前瞻性收集具有可比表型的高质量病例， 拉什阿尔茨海默病中心和纽约脑库（NYBB）。BIO Core的目标是将 临床病理数据与现代分子技术从脑组织，以促进创新 关于衰老和AD的研究BIO核心将（1）通过确定， 准备和分配每个目标的最佳可用样品，（2）确定每个目标的截面平面 样本，（3）从每个样本中收集标准化的结果测量。BIO Core还将支持 空间多组学（SM）核心的创新方法开发， 提供高质量的分子级组织。同时，协调的表型数据将流向 集成计算分析（伊卡）核心，将表型与每个项目的数据相匹配。 将使用由行政核心管理的实验室信息管理系统（LIMS）， 组织和跟踪所有样本特征，实现对样本选择的结构化评估， 准备过程，以发现任何薄弱环节，并确定新的质量措施。总而言之，BIO Core将显著提高本计划生成的数据的质量、注释和可重复性 它的过程和协议将有助于指导和加强组织处理的改进， 神经退行性疾病社区。