Mouse Behavior Phenotyping Core

小鼠行为表型核心--项目总结 神经发育障碍小鼠行为表型核心(MBPC)提供 向NDD调查人员提供设备、专业知识和培训，将老鼠行为评估整合到他们的 研究项目。该核心为调查人员在其研究的各个方面提供支持，包括 就适当的动物模型、畜牧业培训(基因分型、育种)、设计和运行提供咨询 实验、数据分析、展示和解释。MBPC的目标是维护和扩大使用 与NDD相关的行为分析，提供使用多个鼠标行为的技术专业知识 范例，消除进入障碍，并为有兴趣的Cobre调查人员提供详细的培训 在他们的研究中使用了老鼠行为的方法。MBPC包含一系列行为设备，这些设备允许 用于运动、情绪、学习和记忆、社交和其他与NDD相关的表型的表型。这包括 1)使用开阔的场地、运动室和 多水平红外光束阵列、转杆和平衡束；2)多重焦虑分析(高架加、开阔视野、 大理石掩埋、亮/暗盒子)和与情绪有关的测试(蔗糖偏好、强迫游泳、试管测试、新颖性 偏好)和5个选项的连续反应时间脉冲测试；3)设计用于测试工作的、短的、 而长期记忆包括巴恩斯迷宫、T迷宫、恐惧条件反射、恐惧强化惊吓、蔗糖自我 行政管理；4)社会评估，包括社会互动和在开阔场地和三室的偏好 变种，USV通讯测试幼崽和成年幼崽的痛苦和社交电话。此外，MBPC 建议扩大服务范围，包括脑电和遥测监测，以提供有关睡眠、癫痫、 以及使用人工智能辅助姿势估计和Motif的其他生理测量和高级行为分析 身份证明。除了提供培训和获得可用的设备外，新城疫调查人员还 由MBPC动物技术员进行研究的选项。为实现所述目标，MBPC将 由以下三个目标组成：1)维护、扩展和开发与以下相关的行为分析 NDD，2)提供专家技术援助，以设计、执行和分析老鼠行为测试， 3)支持和指导初级调查人员和CNDD成员开发和集成MICE 将行为模型纳入他们的研究计划。

MOUSE BEHAVIORAL PHENOTYPING CORE – PROJECT SUMMARY The COBRE in Neurodevelopmental Disorders Mouse Behavioral Phenotyping Core (MBPC) provides equipment, expertise, and training to NDD investigators integrating mouse behavioral assessment into their research programs. The core provides support for investigators at all points in their studies, including consultations on appropriate animal models, husbandry training (genotyping, breeding), designing and running experiments, data analysis, display, and interpretation. The goal of the MBPC is to maintain and expand the use of behavior assays with relevance to NDD, provide technical expertise in the use of multiple mouse behavior paradigms, and remove barriers to entry and provide detailed training for COBRE investigators interested in using mouse behavior methods in their studies. The MBPC houses an array of behavioral apparatuses that allow for phenotyping of motor, mood, learning and memory, social, and other NDD-relevant phenotypes. This includes 1) assessment of ambulatory, rearing, and stereotypy behaviors using open fields, locomotor chambers with multi-level IR-beam arrays, rotorods, and balance beam; 2) multiple anxiety assays (elevated plus, open field, marble burying, light/dark box) and mood-related tests (sucrose preference, forced swim test, tube test, novelty preference) and 5-choice serial reaction time testing for impulsivity; 3) assays designed to test working, short, and long-term memory including Barnes maze, T-maze, fear conditioning, fear potentiated startle, sucrose self- administration; 4) social assays including social interaction and preference in open field and 3-chamber variations, USV communication assays for distress and social calls in pups and adults. In addition, the MBPC proposes to expand offerings to include EEG and telemetry monitoring to provide information on sleep, seizure, and other physiological measures and advanced behavior analysis using AI-assisted pose estimation and motif identification. In addition to providing training and access to available equipment, NDD investigators also have the option for studies to be run by the MBPC animal technician. To achieve the stated objectives, the MBPC will be composed of three aims as follows: 1) Maintain, expand, and develop behavior assays with relevance to NDD, 2) Provide access to expert technical assistance to design, conduct, and analyze mouse behavior assays, and 3) Support and mentor Junior Investigators and CNDD members as they develop and integrate mouse behavior models into their research programs.