Mouse Behavior Phenotyping Core

小鼠行为表型核心

• 批准号: 10556540
• 负责人: Rachel Penrod-Martin
• 金额: $ 30.18万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10556540
• 关键词: Address; Adult; Animal Model; Animal Technicians; Animals; Anxiety; Behavior; Behavior assessment; Behavioral; Biological Assay; Breeding; Centers of Research Excellence; Child; Communication; Communities; Consultations; Darkness; Data; Data Analyses; Disease; Distress; Electroencephalography; Environment; Equilibrium; Equipment; Etiology; Family; Fright; Functional disorder; Funding; Genetic Models; Genotype; Goals; Grant; Human; Impulsivity; Individual; Infrastructure; Leadership; Learning; Light; Manuscripts; Marble; Measures; Memory; Mentors; Methods; Modeling; Modernization; Monitor; Moods; Morbidity - disease rate; Motor; Mus; Neurodevelopmental Disorder; Output; Patients; Phenotype; Physiological; Preparation; Procedures; Protocols documentation; Reaction Time; Reproducibility; Research; Research Design; Research Personnel; Resources; Running; Scheme; Seizures; Self Administration; Sleep; Social Interaction; Standardization; Structure; Sucrose; Symptoms; Technical Expertise; Technology; Telemetry; Test Result; Testing; Training; Translational Research; Tube; United States National Institutes of Health; Variant; behavior test; behavioral phenotyping; conditioned fear; design; empowerment; experience; experimental study; forced swim test; interest; long term memory; member; mouse genetics; mouse model; neurodevelopment; peer; peer coaching; preference; programs; pup; skills; social; stereotypy

MOUSE BEHAVIORAL PHENOTYPING CORE – PROJECT SUMMARY The COBRE in Neurodevelopmental Disorders Mouse Behavioral Phenotyping Core (MBPC) provides equipment, expertise, and training to NDD investigators integrating mouse behavioral assessment into their research programs. The core provides support for investigators at all points in their studies, including consultations on appropriate animal models, husbandry training (genotyping, breeding), designing and running experiments, data analysis, display, and interpretation. The goal of the MBPC is to maintain and expand the use of behavior assays with relevance to NDD, provide technical expertise in the use of multiple mouse behavior paradigms, and remove barriers to entry and provide detailed training for COBRE investigators interested in using mouse behavior methods in their studies. The MBPC houses an array of behavioral apparatuses that allow for phenotyping of motor, mood, learning and memory, social, and other NDD-relevant phenotypes. This includes 1) assessment of ambulatory, rearing, and stereotypy behaviors using open fields, locomotor chambers with multi-level IR-beam arrays, rotorods, and balance beam; 2) multiple anxiety assays (elevated plus, open field, marble burying, light/dark box) and mood-related tests (sucrose preference, forced swim test, tube test, novelty preference) and 5-choice serial reaction time testing for impulsivity; 3) assays designed to test working, short, and long-term memory including Barnes maze, T-maze, fear conditioning, fear potentiated startle, sucrose self- administration; 4) social assays including social interaction and preference in open field and 3-chamber variations, USV communication assays for distress and social calls in pups and adults. In addition, the MBPC proposes to expand offerings to include EEG and telemetry monitoring to provide information on sleep, seizure, and other physiological measures and advanced behavior analysis using AI-assisted pose estimation and motif identification. In addition to providing training and access to available equipment, NDD investigators also have the option for studies to be run by the MBPC animal technician. To achieve the stated objectives, the MBPC will be composed of three aims as follows: 1) Maintain, expand, and develop behavior assays with relevance to NDD, 2) Provide access to expert technical assistance to design, conduct, and analyze mouse behavior assays, and 3) Support and mentor Junior Investigators and CNDD members as they develop and integrate mouse behavior models into their research programs.

小鼠行为表型核心-项目总结 COBRE在神经发育障碍小鼠行为表型核心（MBPC）提供 设备，专业知识和培训NDD研究人员将小鼠行为评估纳入他们的 研究项目。核心为研究者在研究中的各个方面提供支持，包括 就适当的动物模型、畜牧业培训（基因分型、育种）、设计和运行进行咨询 实验、数据分析、显示和解释。MBPC的目标是保持和扩大使用 与NDD相关的行为测定，提供使用多种小鼠行为的技术专长 范例，并消除进入障碍，并为对COBRE感兴趣的调查人员提供详细的培训， 在他们的研究中使用老鼠行为方法。MBPC容纳了一系列的行为装置， 用于运动、情绪、学习和记忆、社交和其他NDD相关表型的表型分析。这包括 1)使用开放场地、运动室和 多级红外线束阵列、旋转杆和平衡木; 2）多种焦虑测定（升高的正，开放视野， 大理石掩埋、明/暗箱）和情绪相关测试（蔗糖偏好、强迫游泳测试、试管测试、新奇感 偏好）和5-选择系列反应时间测试冲动性; 3）设计用于测试工作，短， 和长时记忆，包括巴恩斯迷宫、T-迷宫、恐惧条件反射、恐惧增强惊吓、蔗糖自我记忆。 4）开放视野和三腔室中的社会性测定，包括社会互动和偏好 变异，USV通信测定的痛苦和社会呼吁在幼崽和成年人。此外，MBPC 提议扩大服务范围，包括EEG和遥感监测，以提供有关睡眠、癫痫发作的信息， 以及其他生理测量和高级行为分析，使用AI辅助的姿势估计和主题 识别.除了提供培训和使用可用设备外，NDD调查人员还 由MBPC动物技术员进行研究的选项。为了实现上述目标，MBPC将 由以下三个目标组成：1）维护，扩展和开发与以下相关的行为测定： NDD，2）提供专家技术援助，以设计、进行和分析小鼠行为测定， 和3）支持和指导初级研究员和CNDD成员，因为他们开发和整合鼠标 行为模型融入到他们的研究项目中。