EXTRACELLULAR MATRIX AND CELL ATTACHMENT PROTEINS

细胞外基质和细胞附着蛋白

• 批准号: 2092408
• 负责人: HAROLD P ERICKSON
• 金额: $ 23.37万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2092408
• 关键词: Caenorhabditis elegans; Drosophilidae; X ray crystallography; affinity chromatography; binding proteins; cell adhesion molecules; embryogenesis; enzyme linked immunosorbent assay; extracellular matrix; gene expression; genetic library; genetically modified animals; immunochemistry; laboratory mouse; laminin; membrane proteins; neoplastic growth; northern blottings; polymerase chain reaction; protein structure function; tissue /cell culture; western blottings; wound healing

In the coming year we plan to pursue 4 lines of research: a) Electron microscopy of thin sections of fibrin. We will use tannic acid fixation in an attempt to visualize the packing of protofibrils in cross-sections of fibrin fibers. b) Electron microscopy of Factor VIII. These studies will be pursued in collaboration with Dr. P.A. McKee's lab for purification and characterization of Factor VIII. c) Electron microscopy of fibronectin. This is a serum protein closely related to the cell surface fibronectin. We have already observed the shape of the molecule (a long flexible chain) and its attachment to fibrinogen molecules. d) Nucleation and assembly of fibrinogen and fibronectin. We will use a combination light scattering and electron microscopy to characterize small polymers of these two proteins, and especially to investigate the question of nucleation of polymerization.

在接下来的一年里，我们计划进行4项研究：a)电子显微镜