EXTRACELLULAR MATRIX AND CELL ATTACHMENT PROTEINS

细胞外基质和细胞附着蛋白

• 批准号: 2092408
• 负责人: HAROLD P ERICKSON
• 金额: $ 23.37万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2092408
• 关键词: Caenorhabditis elegans; Drosophilidae; X ray crystallography; affinity chromatography; binding proteins; cell adhesion molecules; embryogenesis; enzyme linked immunosorbent assay; extracellular matrix; gene expression; genetic library; genetically modified animals; immunochemistry; laboratory mouse; laminin; membrane proteins; neoplastic growth; northern blottings; polymerase chain reaction; protein structure function; tissue /cell culture; western blottings; wound healing

In the coming year we plan to pursue 4 lines of research: a) Electron microscopy of thin sections of fibrin. We will use tannic acid fixation in an attempt to visualize the packing of protofibrils in cross-sections of fibrin fibers. b) Electron microscopy of Factor VIII. These studies will be pursued in collaboration with Dr. P.A. McKee's lab for purification and characterization of Factor VIII. c) Electron microscopy of fibronectin. This is a serum protein closely related to the cell surface fibronectin. We have already observed the shape of the molecule (a long flexible chain) and its attachment to fibrinogen molecules. d) Nucleation and assembly of fibrinogen and fibronectin. We will use a combination light scattering and electron microscopy to characterize small polymers of these two proteins, and especially to investigate the question of nucleation of polymerization.

在来年，我们计划进行4条研究：a）电子显微镜 纤维蛋白的薄部分。 我们将使用单宁酸固定来尝试 可视化原纤维在纤维纤维纤维的横截面中的包装。 b） VIII因子的电子显微镜。 这些研究将在 与P.A.博士合作 麦基的净化和表征实验室 VIII因子。 C）纤连蛋白的电子显微镜。 这是一种血清蛋白 与细胞表面纤连蛋白密切相关。 我们已经观察到 分子的形状（长柔性链）及其附着在纤维蛋白原上 分子。 d）纤维蛋白原和纤连蛋白的成核和组装。 我们将 使用组合光散射和电子显微镜表征小 这两种蛋白质的聚合物，尤其是为了研究 聚合的成核。