CONTACT BEHAVIOR OF DEVELOPING AND TRANSFORMED CELLS

这项研究的总体目标是了解正常组织是如何 细胞和癌细胞在生物体内移动。重点放在：(1) 胚胎和成体内细胞运动的机制，特别是 在定向形态发生运动和伤口闭合过程中，以及(2) 侵袭性癌细胞的运动机制--它们如何接触 与彼此，与正常组织细胞，以及与各种底物，以及 这种行为与获取侵入性有何关系。这两个 重点内容都与构成单元格的属性有关 运动：附着在底物上(无论它是否由其他细胞组成 或细胞外基质)，细胞表面的突起，脱离， 以及后缘、细胞表面流动和质膜的回缩 组装、细胞质流动、微丝和细胞质收缩， 微管和细胞质粘度，以及接触抑制关系 在细胞之间。将使用的主要技术是CELL 培养，高分辨率Nomarski干涉，相位对比和 干涉反射显微镜、延时电影显微摄影术、 透射和扫描电子显微镜、微操作、药物 抑制微管组装、收缩和能量代谢 以及用免疫荧光抗体标记收缩蛋白。(A)

The overall objective of this research is to understand how normal tissue cells and cancer cells move within organisms. The emphasis is on: (1)\the mechanism of cell locomotion within both embryos and adults, particularly during directional morphogenetic movements and wound closure, and (2)\the mechanism of locomotion of invasive cancer cells--how they make contact with each other, with normal tissue cells, and with various substrata, and how this behavior relates to the acquisition of invasiveness. These two lines of emphasis are both concerned with the properties that make for cell locomotion: adhesion to the substratum (whether it consists of other cells or of extracellular matrix), protrusions of the cell surface, detachment, and retraction of the trailing edge, cell surface flow and plasma membrane assembly, cytoplasmic flow, microfilaments, and cytoplasmic contractility, microtubules and cytoplasmic viscosity, and contact inhibitory relations between cells. The principal techniques that will be utilized are cell culture, high-resolution Nomarski interference, phase contrast and interference reflection microscopy, time-lapse cinemicrography, transmission and scanning electron microscopy, micromanipulation, drug inhibition of microtubule assembly, contractility, and energy metabolism and labeling of contractile proteins with immunofluorescent antibodies. (A)