AGING-SPECIFIC GENE EXPRESSION IN DROSOPHILA

为了开始理解衰老的生物学，我们建议识别基因 它们在衰老过程中差异表达，并决定 这种监管机制。 热休克蛋白70（hsp 7 O）基因 发现在衰老过程中， 苍蝇，这种诱导被发现需要以前未确定的 启动子元件 这些相同的新元件也可能介导hsp 7 O 由氧自由基诱导。 我们建议详细分析 启动子序列和反式作用因子， 热休克蛋白7 O表达及其与氧自由基的关系 生产 为了鉴定具有衰老特异性表达模式的其他基因，我们 分析了600个含有转座子的果蝇品系 称为“增强子陷阱”，其充当表达的报告子。 基因的模式 12条线表现出各种老化和组织特异性 表达模式 这些增强子所识别的基因- 陷阱将被识别，克隆和分析，目标是 了解基因的组织特异性变化的机制 衰老过程中的表达

To begin to understand the biology of aging, we propose to identify genes which are differentially expressed during aging, and determine the mechanisms for this regulation. The heat shock protein 70 (hsp7O) gene was found to be induced in muscle during aging, and using transgenic flies, this induction was found to require previously unidentified promoter elements. These same novel elements may also mediate hsp7O induction by oxygen radicals. We propose to analyze in detail the promoter sequences and trans-acting factors which regulate aging-specific hsp7O expression, and their possible relationship to oxygen radical production. To identify additional genes with aging-specific expression patterns, we have analyzed 600 fly lines containing genetically engineered transposons called "enhancer-traps", which act as reporters for the expression patterns of genes. 12 lines exhibit various aging- and tissue-specific expression patterns. The genes identified by several of these enhancer- traps will be identified, cloned and analyzed, with the goal of understanding the mechanisms for tissue-specific changes in gene expression during aging.