CLASS II GENES IN AUTOIMMUNE ANIMALS
CLASS II GENES IN AUTOIMMUNE ANIMALS
批准号:
2063808
负责人:
JOEL SCHIFFENBAUER
金额:
$18.11万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-09-01 至 1995-07-31
关键词:
DNA MHC class II antigen autoimmunity electrophoresis genetic library genetic manipulation genetic regulatory element genetically modified animals laboratory mouse monoclonal antibody nucleic acid hybridization protein sequence protein structure function transcription factor transfection western blottings
中文摘要
II类分子在正常和异常中都起着核心作用
免疫反应和一些自身免疫性疾病
老鼠似乎与第二类分子相连。我们建议评估
II类分子的定性和定量贡献
从NZW小鼠品系到(NZWxNZB)F小鼠的自身免疫。这个
分子中II类分子的定性结构功能关系
自身免疫性将通过寻找NZW基因中的不寻常序列来评估。
我们将开发针对NZW/NZB的单抗
杂化II类分子来检查杂化II类分子
,并确定这些杂化分子是否可能对
自身免疫的发展。使用转基因技术,我们还将
确定“u”单倍型是否是免疫显性的,这将有助于
解释NZW对加速自身免疫的贡献。此外,我们
计划生产转基因NZB小鼠,其中NZW II类转基因
放入NZB小鼠体内。
评估是否存在II类的数量贡献
我们将分离NZB和NZW基因并检测
调节类基因表达的顺式和反式作用因子
II基因。顺位作用因素将通过确定是否
NZW和NZB的II类基因5‘侧翼区有异常
序列。编码反式作用因子的基因将被分离出来
西南方法学,由此标记的寡核苷酸对应于
NZB和NZW 5‘调控区用于探测NZB和NZW lambda
Gt11文库。这些cdna将被检查以寻找不寻常的序列。
这些研究将对定量和定性两方面进行评估
第二类基因对自身免疫的贡献。
英文摘要
The class II molecules play a central role in both normal and abnormal
immune responses, and a number of autoimmune diseases both in man and
mouse appear to be linked to class II molecules. We propose to evaluate
the qualitative and quantitative contribution of the class II molecules
from the NZW mouse strain to autoimmunity in the (NZWxNZB)F mouse. The
qualitative structure function relationship of class II molecules in
autoimmunity will be evaluated by seeking unusual sequences in NZW cDNA's.
We will develop monoclonal antibodies specific for the NZW/NZB
hybrid class II molecules to examine whether hybrid class II molecules
exist in vivo, and determine if these hybrid molecules might contribute to
the development of autoimmunity. Using transfections we will also
determine whether the "u" haplotype is immunodominant, which would help
explain the NZW contribution to accelerated autoimmunity. In addition, we
plan to produce transgenic NZB mice, in which NZW class II transgenes are
placed into NZB mice.
To evaluate whether there is a quantitative contribution of class II
molecules to autoimmunity, we will isolate NZB and NZW genes and examine
both cis and trans acting factors which regulate the expression of class
II genes. Cis acting factors will be evaluated by determining whether the
5' flanking region of the class II genes of NZW and NZB have unusual
sequences. cDNA's encoding trans acting factors will be isolated by a
Southwestern methodology whereby labeled oligonucleotides corresponding to
the NZB and NZW 5' regulatory regions are used to probe NZB and NZW lambda
gt11 libraries. These cDNA's will be examined for unusual sequences.
These studies will evaluate both the quantitative and qualitative
contributions of class II genes to autoimmunity.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Hyperproliferation of BXSB B cells is linked to the Yaa allele.
BXSB B 细胞的过度增殖与 Yaa 等位基因有关。
DOI:
10.1006/clin.1996.0170
发表时间:
1996
期刊:
Clinical immunology and immunopathology
影响因子:
--
作者:
[DesJardin,LE, Butfiloski,EJ, Sobel,ES, Schiffenbauer,J]
通讯作者:
Schiffenbauer,J
Sequence of class II major histocompatibility complex genes from MRL mice. Conserved amino acids in the I-E beta chains from autoimmune mice.
MRL 小鼠的 II 类主要组织相容性复合体基因序列。
DOI:
10.1002/art.1780341111
发表时间:
1991
期刊:
Arthritis and rheumatism
影响因子:
--
作者:
[Schiffenbauer,J, Wegrzyn,L]
通讯作者:
Wegrzyn,L
Background genes mediate the development of autoimmunity in (NZB x PL/J)F1 or (NZB x BIO.PL)F1 mice.
背景基因介导 (NZB x PL/J)F1 或 (NZB x BIO.PL)F1 小鼠自身免疫的发展。
DOI:
10.1016/0090-1229(92)90076-z
发表时间:
1992
期刊:
Clinical immunology and immunopathology
影响因子:
--
作者:
[Schiffenbauer,J, Wegrzyn,L, Croker,BP]
通讯作者:
Croker,BP
Autoimmune toxicity of chlorinated compounds
-
批准号:6664570
-
项目类别:
-
资助金额:$13.16万
-
财政年份:2002
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
Autoimmune toxicity of chlorinated compounds
-
批准号:6580398
-
项目类别:
-
资助金额:$13.16万
-
财政年份:2002
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
Autoimmune toxicity of chlorinated compounds
-
批准号:6443387
-
项目类别:
-
资助金额:$13.16万
-
财政年份:2001
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
GENETIC LOCI CONTRIBUTING TO AUTOIMMUNITY IN BXSB MICE
-
批准号:6201320
-
项目类别:
-
资助金额:$15.69万
-
财政年份:1999
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
GENETIC LOCI CONTRIBUTING TO AUTOIMMUNITY IN BXSB MICE
-
批准号:6100098
-
项目类别:
-
资助金额:$0.0万
-
财政年份:1998
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
GENETIC LOCI CONTRIBUTING TO AUTOIMMUNITY IN BXSB MICE
-
批准号:6268254
-
项目类别:
-
资助金额:$14.93万
-
财政年份:1998
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
GENETIC LOCI CONTRIBUTING TO AUTOIMMUNITY IN BXSB MICE
-
批准号:6235517
-
项目类别:
-
资助金额:$14.36万
-
财政年份:1997
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
ENVIONMENTAL AGENTS IN THE INDUCTION OF AUTOIMMUNITY
-
批准号:2018976
-
项目类别:
-
资助金额:$7.25万
-
财政年份:1996
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
Autoimmune toxicity of chlorinated compounds
-
批准号:6330919
-
项目类别:
-
资助金额:$13.16万
-
财政年份:1995
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
CLASS II GENES IN AUTOIMMUNE RODENTS
-
批准号:3141525
-
项目类别:
-
资助金额:$15.34万
-
财政年份:1990
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
CLASS II GENES IN AUTOIMMUNE RODENTS
-
批准号:3141526
-
项目类别:
-
资助金额:$16.74万
-
财政年份:1990
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
CLASS II GENES IN AUTOIMMUNE RODENTS
-
批准号:3141527
-
项目类别:
-
资助金额:$18.46万
-
财政年份:1990
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
CLASS II GENES IN AUTOIMMUNE RODENTS
-
批准号:3141528
-
项目类别:
-
资助金额:$18.11万
-
财政年份:1990
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:
GENETIC LOCI CONTRIBUTING TO AUTOIMMUNITY IN BXSB MICE
-
批准号:5206018
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:JOEL SCHIFFENBAUER
-
依托单位:--
海外基金