Singlestage non-TransgEnic crispr for crop Productivity (STEP)

到2050年，作物产量必须提高70%，以满足全球人口的增长（世卫组织，2016年）。基因组编辑（GE）通过创造新的植物性状来提高生产力。单个物种使用该技术的费用在800万至8000万英镑之间（AgFunder, 2016）。I是一个复杂的两阶段的产生过程，其中第二阶段（非转基因）特别漫长。所有作物都有患病风险，例如香蕉黑叶斑病（BSD）导致50%的产量损失，迫使农民每年使用杀菌剂20-70次（公平贸易，2016年）。因此，为了让更多的物种获得非转基因转基因，比如无性作物和育种周期长的作物，这个过程需要更快、更便宜（Economist, 2016）。热带生物科学公司（TBio）创造了一种非转基因转基因（NTC）工具，这是一种单阶段的转基因过程，这意味着它可以应用于长育种周期和无性作物。他们的目标是在实验室条件下证明这一概念，用抗bsd香蕉特性来证明它，将GE过程的时间和成本减少70%，并使他们的NTC工具能够负担得起小型创新者的许可。

Crop productivity must increase 70% by 2050 to meet rising global population (WHO, 2016). Genome Editing (GE) increases productivity by creating new plant traits. Payments to use the technology for a single species range from £8m-80m (AgFunder, 2016). I is a complex 2-stage generation process, with the 2nd stage (non-transgenic GE) especially lengthy. All crops are at risk of disease, eg Black Sigatoka Disease (BSD) causes 50% yield losses in banana, obliging farmers to use fungicide 20-70 times/yr (Fairtrade, 2016). Thus, to make non-transgenic GE available to more species, such as asexual crops and crops with long breeding cycles the process needs to be faster and cheaper (Economist, 2016). Tropic Biosciences (TBio) has created a Non-Transgenic GE (NTC) Tool, a single-stage only generation GE process, meaning it can be applied to long breeding cycle and asexual crops. Their objective is to prove the concept in lab conditions, demonstrate it with a BSD-resistant banana trait, reduce the GE process time and cost by 70% and make their NTC Tool affordably licensable to small innovators.