Singlestage non-TransgEnic crispr for crop Productivity (STEP)

到2050年，作物生产力必须提高70%，以满足全球人口增长的需求（WHO，2016）。基因组编辑（GE）通过创造新的植物性状来提高生产力。将该技术用于单一物种的费用从800万英镑到8000万英镑不等（AgFunder，2016）。I是一个复杂的2阶段生成过程，第2阶段（非转基因GE）特别漫长。所有作物都面临着疾病的风险，例如黑色香蕉叶斑病（BSD）导致香蕉产量损失50%，迫使农民每年使用20-70次杀菌剂（Fairtrade，2016）。因此，为了使非转基因转基因技术能够应用于更多物种，如无性繁殖作物和育种周期长的作物，这一过程需要更快、更便宜（经济学人，2016）。Tropic Biosciences（TBio）创建了一种非转基因GE（NTC）工具，这是一种单阶段的遗传工程方法，这意味着它可以应用于长育种周期和无性繁殖作物。他们的目标是在实验室条件下证明这一概念，用抗BSD的香蕉性状来证明这一点，将GE的加工时间和成本减少70%，并使他们的NTC工具能够负担得起小型创新者的许可。

Crop productivity must increase 70% by 2050 to meet rising global population (WHO, 2016). Genome Editing (GE) increases productivity by creating new plant traits. Payments to use the technology for a single species range from £8m-80m (AgFunder, 2016). I is a complex 2-stage generation process, with the 2nd stage (non-transgenic GE) especially lengthy. All crops are at risk of disease, eg Black Sigatoka Disease (BSD) causes 50% yield losses in banana, obliging farmers to use fungicide 20-70 times/yr (Fairtrade, 2016). Thus, to make non-transgenic GE available to more species, such as asexual crops and crops with long breeding cycles the process needs to be faster and cheaper (Economist, 2016). Tropic Biosciences (TBio) has created a Non-Transgenic GE (NTC) Tool, a single-stage only generation GE process, meaning it can be applied to long breeding cycle and asexual crops. Their objective is to prove the concept in lab conditions, demonstrate it with a BSD-resistant banana trait, reduce the GE process time and cost by 70% and make their NTC Tool affordably licensable to small innovators.