Clay nanoparticle-facilitated RNAi for non-transgenic modification of crops

粘土纳米颗粒促进的 RNAi 用于作物的非转基因修饰

• 批准号: DP190103486
• 负责人: Prof Zhi Ping Xu
• 金额: $ 31.6万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-10 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190103486
• 关键词: Clay; nanoparticle; facilitated; RNAi; non

This project aims to define the most effective spray formulations, consisting of clay nanoparticles and induced RNA interference (RNAi) to manipulate gene expression in plants. Topical application of double-stranded RNA (dsRNA) for RNAi represents an attractive alternative to genetically engineered crops. However, naked dsRNA is unstable and is not efficiently taken up by plants. For these reasons, topical application of dsRNA has thus far produced only modest induction of RNAi in plants. Nanoparticle-facilitated manipulation of gene expression in plants will enable sustainable clean green strategies for protecting crops from diseases. This project will result in improved crop protection and productivity and boost the export potential of Australian crops.

该项目旨在定义最有效的喷雾制剂，由粘土纳米颗粒和诱导的RNA干扰（RNAi）组成，以操纵植物中的基因表达。用于RNAi的双链RNA（dsRNA）的局部应用代表了基因工程作物的有吸引力的替代方案。然而，裸dsRNA是不稳定的，不能被植物有效吸收。由于这些原因，dsRNA的局部施用迄今为止仅在植物中产生适度的RNAi诱导。纳米粒子促进植物基因表达的操纵将使可持续的清洁绿色策略成为可能，以保护作物免受疾病的侵害。该项目将改善作物保护和生产力，并提高澳大利亚农作物的出口潜力。