ISOTYPE SWITCHING IN A NEOPLASTIC B-CELL MODEL BCL 1
肿瘤 B 细胞模型 BCL 1 中的同种型转换
基本信息
- 批准号:2088142
- 负责人:
- 金额:$ 23.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1982
- 资助国家:美国
- 起止时间:1982-03-01 至 1995-04-30
- 项目状态:已结题
- 来源:
- 关键词:B lymphocyte DNA binding protein DNA replication cell differentiation cellular oncology complementary DNA disease /disorder model gene expression gene rearrangement genetic mapping genetic transcription hybridomas immunogenetics immunoglobulin genes interleukin 5 interleukin 6 laboratory mouse laboratory rabbit lymphocytic leukemia lymphokines molecular cloning neoplasm /cancer genetics neoplasm /cancer immunology nucleic acid sequence protein biosynthesis tissue /cell culture transfection
项目摘要
This grant has supported studies on various aspects of class switching and
lymphokine induction in the spontaneous murine B leukemia, BCL1. Our
findings during the last period have prompted a broader investigation of
B cell differentiation using this model system and normal B cells. Based
on significant supporting data, we have condensed our continuing efforts
into three projects. First, we want to determine the precise mechanism and
its control for allelically excluded, dual synthesis of mu and gamma1
chains in BCL1 subclones. We know that this occurs by some form of
discontinuous transcription, and not by production of a 'long transcript'
covering the entire VDJ to C-gamma1 region. Second, we want to determine
precisely how IL-5 and IL-6 in conjunction with antigen induce increased
rates of u transcription and DNA replication in BCL1 cells rendered
antigen-specific by transfection. We have identified the target sequences
and the protein-DNA interactions for IL-5+Ag. We want to do the same with
IL-6 plus go after the factors induced by both lymphokines. Third, we want
to study proteins expressed only at the immature/mature B cell
differentiation stage based on the premise that functions relevant to Ig
gene expression will be discovered. We have cloned from BCL1 two mRNAs that
satisfy the criteria of cell type- and stage-specificity.
这项拨款资助了有关转班的各个方面的研究,
自发性小鼠B白血病中的淋巴因子诱导,BCL 1。我们
上一个时期的调查结果促使对
使用该模型系统的B细胞分化和正常B细胞。基于
在重要的支持数据上,我们浓缩了我们持续的努力,
分为三个项目。首先,我们要确定确切的机制,
其对Mu和γ 1等位基因排斥的双重合成的控制
BCL 1亚克隆中的链。我们知道这是通过某种形式的
不连续转录,而不是通过产生“长转录本”
覆盖整个VDJ到C-γ 1区域。第二,我们要确定
IL-5和IL-6如何与抗原联合诱导增加
在BCL 1细胞中的u转录和DNA复制率呈现
抗原特异性转染。我们已经确定了目标序列
IL-5+Ag的蛋白质-DNA相互作用。我们想做同样的事情,
IL-6加IL-6后,两种淋巴因子诱导的因子。第三,我们要
研究仅在未成熟/成熟B细胞中表达的蛋白质
分化阶段基于与IG相关的功能
基因表达将被发现。我们从BCL 1中克隆了两种mRNA,
满足细胞类型和阶段特异性的标准。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('HALEY O TUCKER', 18)}}的其他基金
A TRANSCRIPTION FACTOR WITHIN LIPID RAFTS MODULATES B CELL SIGNALING VIA THE BCR
脂筏内的转录因子通过 BCR 调节 B 细胞信号传导
- 批准号:
7849906 - 财政年份:2009
- 资助金额:
$ 23.86万 - 项目类别:
ROLE OF eARIDs IN LYMPHOCYTE FUNCTION AND DEVELOPMENT
EARID 在淋巴细胞功能和发育中的作用
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6762317 - 财政年份:2004
- 资助金额:
$ 23.86万 - 项目类别:
ROLE OF eARIDs IN LYMPHOCYTE FUNCTION AND DEVELOPMENT
EARID 在淋巴细胞功能和发育中的作用
- 批准号:
7347004 - 财政年份:2004
- 资助金额:
$ 23.86万 - 项目类别:
ROLE OF eARIDs IN LYMPHOCYTE FUNCTION AND DEVELOPMENT
EARID 在淋巴细胞功能和发育中的作用
- 批准号:
7009961 - 财政年份:2004
- 资助金额:
$ 23.86万 - 项目类别:
ROLE OF eARIDs IN LYMPHOCYTE FUNCTION AND DEVELOPMENT
EARID 在淋巴细胞功能和发育中的作用
- 批准号:
7176203 - 财政年份:2004
- 资助金额:
$ 23.86万 - 项目类别:
ROLE OF eARIDs IN LYMPHOCYTE FUNCTION AND DEVELOPMENT
EARID 在淋巴细胞功能和发育中的作用
- 批准号:
6929261 - 财政年份:2004
- 资助金额:
$ 23.86万 - 项目类别:
Role of Bop in Cardiac Development and Function
BOP 在心脏发育和功能中的作用
- 批准号:
6744117 - 财政年份:2003
- 资助金额:
$ 23.86万 - 项目类别:
Role of Bop in Cardiac Development and Function
BOP 在心脏发育和功能中的作用
- 批准号:
6874501 - 财政年份:2003
- 资助金额:
$ 23.86万 - 项目类别:
Role of Bop in Cardiac Development and Function
BOP 在心脏发育和功能中的作用
- 批准号:
7054705 - 财政年份:2003
- 资助金额:
$ 23.86万 - 项目类别:
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正常和恶性 B 细胞发育中的 BCL11 基因
- 批准号:
6364329 - 财政年份:2001
- 资助金额:
$ 23.86万 - 项目类别:
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