DNA HELICASES AND REPLICATION OF BOVINE PAPILLOMA VIRUS
牛乳头瘤病毒的 DNA 解旋酶和复制
基本信息
- 批准号:2097379
- 负责人:
- 金额:$ 27.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-04-01 至 1997-01-31
- 项目状态:已结题
- 来源:
- 关键词:DNA binding protein DNA footprinting DNA replication DNA replication origin DNA topoisomerases HeLa cells affinity chromatography biological models bovine papillomavirus cell cycle cell cycle proteins enzyme mechanism gel electrophoresis gel mobility shift assay helicase mutant open reading frames phosphorylation posttranslational modifications protein purification protein sequence protein structure scanning transmission electron microscopy virus DNA
项目摘要
The replication of duplex DNA requires the formation of single stranded
DNA in order to support Watson-Crick base pairing of entering nucleotides
in the synthesis of daughter strands. DNA helicases are enzymes which
lead to the unwinding of duplex DNA and are found at the replication
fork. The long-term objective of this proposal is to identify and
characterize DNA helicases that are important in the replication of DNA.
The proposal includes the identification, isolation and characterization
of DNA helicases that are dependent on the human DNA binding protein for
their activity, The three subunit human DNA binding protein is the only
binding protein which supports the replication of DNA containing the SV40
origin. This binding protein was also shown to be required for the
repair of alkylated or ultraviolet damaged DNA.
The replication of Bovine Papilloma virus DNA in vitro by mouse cell free
extracts depends upon the viral encoded proteins El and E2. The El
protein has been shown to contain DNA helicase activity and binds to the
minimal BPV origin of replication. We plan to investigate the mechanism
by which the replication of this DNA is regulated. We shall examine the
cell cycle control of the unwinding reaction dependent on the BPV core
origin catalyzed by the El protein.
The regulation of DNA replication is of considerable importance in normal
cells. The information derived from the studies described here may
contribute to our knowledge of how cancer cells escape this control.
双链DNA的复制需要形成单链
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jerard Hurwitz其他文献
Jerard Hurwitz的其他文献
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{{ truncateString('Jerard Hurwitz', 18)}}的其他基金
Studies with the human Cdc45-Mcm2-7-GINS helicase complex
人类 Cdc45-Mcm2-7-GINS 解旋酶复合物的研究
- 批准号:
8671152 - 财政年份:2014
- 资助金额:
$ 27.38万 - 项目类别:
Studies on the Checkpoint Regulation of DNA Synthesis
DNA合成检查点调控的研究
- 批准号:
6421479 - 财政年份:2002
- 资助金额:
$ 27.38万 - 项目类别:
Studies on the Checkpoint Regulation of DNA Synthesis
DNA合成检查点调控的研究
- 批准号:
6696966 - 财政年份:2002
- 资助金额:
$ 27.38万 - 项目类别:
Studies on the Checkpoint Regulation of DNA Synthesis
DNA合成检查点调控的研究
- 批准号:
6620749 - 财政年份:2002
- 资助金额:
$ 27.38万 - 项目类别:
Studies on the Checkpoint Regulation of DNA Synthesis
DNA合成检查点调控的研究
- 批准号:
6844860 - 财政年份:2002
- 资助金额:
$ 27.38万 - 项目类别:
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- 批准号:
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