TRANSFORMING GENES OF AVIAN SARCOMA VIRUSES

转化禽肉瘤病毒的基因

基本信息

  • 批准号:
    3168663
  • 负责人:
  • 金额:
    $ 16.42万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1981
  • 资助国家:
    美国
  • 起止时间:
    1981-02-01 至 1992-01-31
  • 项目状态:
    已结题

项目摘要

The objective of this proposal is to understand the nature and functions of the transforming genes of avian sarcoma viruses (ASVs) and their normal cellular counter-part proto-oncogenes. The transforming gene of ASV UR2, v-ros, its corresponding cellular gene, c-ros, and the proto-oncogene c-src will be studied. The functional domains of v-ros will be studied by constructing various site-specific mutants, and ros and src recombinants. Synthesis and subcellular location of the ros protein, its interaction with potential cellular substrates and transforming ability will be compared among parental UR2 and the mutants. The expression of c-ros gene in avian and mammalian tissues at various developmental stages will be examined at RNA and protein levels. The c-ros product will be identified and characterized using ros-specific antisera. In situ immunofluorescence will be performed to identify the specific cells in the tissue expressing the c-ros protein. The cDNA clone of c-ros mRNA will be constructed and sequenced to elucidate the complete c-ros product, which appears to mimic growth factor receptor molecules. To identify the sequence differences between c-ros and v-ros that may be responsible for the different transforming potential of the two genes, c-ros virus and c-ros recombinants will be constructed and compared with respect to their gene products and oncogenicity. Chicken c-src is known to direct the synthesis of a 4 kb mRNA which codes for a 60,000 dalton tyrosine-specific protein kinase. In muscle, while the 4 kb RNA is absent, a 3 kb c-src mRNA lacking most the kinase domain is expressed instead. Both the 4 kb and the 3 kb RNAs will be cloned and sequence to elucidate their nature of synthesis. The potential protein product of the 3 kb RNA will be identified by using antisera against peptides decoded from the nucleotide sequence. The role of this src- related protein in muscle development and function will be studied.
本提案的目的是了解自然和

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

LU-HAI WANG其他文献

LU-HAI WANG的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('LU-HAI WANG', 18)}}的其他基金

Anti-tumor mechanism of a dietary and herbal mixture
饮食和草药混合物的抗肿瘤机制
  • 批准号:
    6818852
  • 财政年份:
    2004
  • 资助金额:
    $ 16.42万
  • 项目类别:
Anti-tumor mechanism of a dietary and herbal mixture
饮食和草药混合物的抗肿瘤机制
  • 批准号:
    6947926
  • 财政年份:
    2004
  • 资助金额:
    $ 16.42万
  • 项目类别:
SYMPOSIUM--ONCOGENES AND CELL SIGNALING
研讨会--癌基因和细胞信号转导
  • 批准号:
    2377300
  • 财政年份:
    1997
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
  • 批准号:
    2837652
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
  • 批准号:
    2470509
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
  • 批准号:
    3199489
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
  • 批准号:
    2096289
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
  • 批准号:
    2096288
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
  • 批准号:
    2096287
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
  • 批准号:
    6475785
  • 财政年份:
    1992
  • 资助金额:
    $ 16.42万
  • 项目类别:

相似海外基金

CRYSTALLOGRAPHY OF N TERMINAL DOMAIN OF ROUS SARCOMA VIRUS CAPSID PROTEIN: HIV
劳斯肉瘤病毒衣壳蛋白 N 末端结构域的晶体学:HIV
  • 批准号:
    6483466
  • 财政年份:
    2001
  • 资助金额:
    $ 16.42万
  • 项目类别:
CRYSTALLOGRAPHY OF N TERMINAL DOMAIN OF ROUS SARCOMA VIRUS CAPSID PROTEIN: HIV
劳斯肉瘤病毒衣壳蛋白 N 末端结构域的晶体学:HIV
  • 批准号:
    6339290
  • 财政年份:
    2000
  • 资助金额:
    $ 16.42万
  • 项目类别:
CRYSTALLOGRAPHY OF N TERMINAL DOMAIN OF ROUS SARCOMA VIRUS CAPSID PROTEIN: HIV
劳斯肉瘤病毒衣壳蛋白 N 末端结构域的晶体学:HIV
  • 批准号:
    6315670
  • 财政年份:
    1999
  • 资助金额:
    $ 16.42万
  • 项目类别:
CRYSTAL STRUCTURE OF CATALYTIC CORE FRAGMENT OF ROUS SARCOMA VIRUS INTEGRASE
劳斯肉瘤病毒整合酶催化核心片段的晶体结构
  • 批准号:
    6120546
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA SPLICING CONTROL IN ROUS SARCOMA VIRUS
劳斯肉瘤病毒中的 RNA 剪接控制
  • 批准号:
    6513153
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA Splicing Control in Rous Sarcoma Virus
劳斯肉瘤病毒中的 RNA 剪接控制
  • 批准号:
    7895522
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA SPLICING CONTROL IN ROUS SARCOMA VIRUS
劳斯肉瘤病毒中的 RNA 剪接控制
  • 批准号:
    6377180
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA SPLICING CONTROL IN ROUS SARCOMA VIRUS
劳斯肉瘤病毒中的 RNA 剪接控制
  • 批准号:
    2896626
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA Signaling Control in Rous Sarcoma Virus
劳斯肉瘤病毒中的 RNA 信号传导控制
  • 批准号:
    6951420
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
RNA Splicing Control in Rous Sarcoma Virus
劳斯肉瘤病毒中的 RNA 剪接控制
  • 批准号:
    7580324
  • 财政年份:
    1998
  • 资助金额:
    $ 16.42万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了