TRANSFORMING GENES OF AVIAN SARCOMA VIRUSES
转化禽肉瘤病毒的基因
基本信息
- 批准号:3168663
- 负责人:
- 金额:$ 16.42万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1981
- 资助国家:美国
- 起止时间:1981-02-01 至 1992-01-31
- 项目状态:已结题
- 来源:
- 关键词:Retroviridae disease Rous sarcoma virus antiserum autoradiography avian sarcoma virus chickens chromosome translocation complementary DNA gel electrophoresis gene expression genetic manipulation genetic mapping immunofluorescence technique laboratory mouse laboratory rabbit laboratory rat molecular cloning molecular oncology mutant nucleic acid sequence oncogenes oncogenic virus point mutation protein tyrosine kinase protooncogene viral carcinogenesis virus RNA virus genetics virus protein
项目摘要
The objective of this proposal is to understand the nature and
functions of the transforming genes of avian sarcoma viruses
(ASVs) and their normal cellular counter-part proto-oncogenes.
The transforming gene of ASV UR2, v-ros, its corresponding
cellular gene, c-ros, and the proto-oncogene c-src will be studied.
The functional domains of v-ros will be studied by constructing
various site-specific mutants, and ros and src recombinants.
Synthesis and subcellular location of the ros protein, its
interaction with potential cellular substrates and transforming
ability will be compared among parental UR2 and the mutants.
The expression of c-ros gene in avian and mammalian tissues at
various developmental stages will be examined at RNA and
protein levels. The c-ros product will be identified and
characterized using ros-specific antisera. In situ
immunofluorescence will be performed to identify the specific
cells in the tissue expressing the c-ros protein. The cDNA clone
of c-ros mRNA will be constructed and sequenced to elucidate the
complete c-ros product, which appears to mimic growth factor
receptor molecules. To identify the sequence differences
between c-ros and v-ros that may be responsible for the different
transforming potential of the two genes, c-ros virus and c-ros
recombinants will be constructed and compared with respect to
their gene products and oncogenicity.
Chicken c-src is known to direct the synthesis of a 4 kb mRNA
which codes for a 60,000 dalton tyrosine-specific protein kinase.
In muscle, while the 4 kb RNA is absent, a 3 kb c-src mRNA
lacking most the kinase domain is expressed instead. Both the 4
kb and the 3 kb RNAs will be cloned and sequence to elucidate
their nature of synthesis. The potential protein product of the 3
kb RNA will be identified by using antisera against peptides
decoded from the nucleotide sequence. The role of this src-
related protein in muscle development and function will be
studied.
本提案的目的是了解自然和
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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LU-HAI WANG其他文献
LU-HAI WANG的其他文献
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{{ truncateString('LU-HAI WANG', 18)}}的其他基金
Anti-tumor mechanism of a dietary and herbal mixture
饮食和草药混合物的抗肿瘤机制
- 批准号:
6818852 - 财政年份:2004
- 资助金额:
$ 16.42万 - 项目类别:
Anti-tumor mechanism of a dietary and herbal mixture
饮食和草药混合物的抗肿瘤机制
- 批准号:
6947926 - 财政年份:2004
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
- 批准号:
2837652 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
- 批准号:
2470509 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
- 批准号:
3199489 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
- 批准号:
2096289 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
- 批准号:
2096288 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS
胰岛素和 IGFI 受体的细胞转化
- 批准号:
2096287 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
CELL TRANSFORMATION BY INSULIN AND IGF1 RECEPTORS
胰岛素和 IGF1 受体的细胞转化
- 批准号:
6475785 - 财政年份:1992
- 资助金额:
$ 16.42万 - 项目类别:
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