GENETIC & BIOCHEMICAL REGULATION OF PP60-V-SRC ACTIVITY
基因
基本信息
- 批准号:3197639
- 负责人:
- 金额:$ 13.89万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-07-10 至 1995-06-30
- 项目状态:已结题
- 来源:
- 关键词:alleles cell cycle chickens laboratory mouse laboratory rabbit molecular cloning molecular genetics molecular oncology mutant neoplastic transformation nucleic acid sequence oncogenes phosphorylation protein biosynthesis protein tyrosine kinase protooncogene site directed mutagenesis transposon /insertion element
项目摘要
The long-term goal of these studies is to identify biochemical steps unique
to the transformed cell which might be amenable to interference by specific
pharmacological agents. Experiments are proposed which will help unravel
the cell's genetic and biochemical contributions to the process of
neoplastic transformation induced by the viral oncogene v-src. Cancer is
an intrinsically complex phenomenon which is poorly understood at the
molecular level. The current lack of understanding at the molecular level
has prevented such a directed pharmacological approach; commonly used drugs
attack all dividing cells, limiting the dose employed and inducing serious
side effects.
Three particular sets of experiments are proposed: (1) experiments
involving further characterization of the unique mutant allele v-src-L, and
the subsequent manipulation of v-src-L by further mutation; (2) experiments
to test the role of mitotic specific phosphorylations in the process of
transformation by v-src; and (3) (in collaboration with Dr. Harold Varmus,
UCSF) analysis of a unique set of mutant of v-src.
v-src-L transforms chicken but not rat cells; it is host-range dependent
for transformation. The pp60v-src-L protein encoded by v-src-L will be
characterized in detail for the Km and Vmax of the intrinsic tyrosine-
specific protein kinase activity against several substrates; preliminary
evidence suggests it exhibits a host-dependent substrate specificity (a
unique property among all oncogenes reported thus far). Proposed
biochemical and genetic experiments will address how this host-dependent
regulation is accomplished. Further experiments will identify the relevant
chicken and rat cellular genes involved in this regulation.
Site-directed mutagenesis will be used to analyze the necessity of mitosis-
specific phosphorylation of wt pp60v-src for (a) increased kinase activity
associated with pp60v-src during mitosis and (b) transformation by v-src.
Work by others in this field has focused on pp60c-src.
Finally, a collection of naturally occurring, biologically selected v-src
alleles (generated by H. Varmus) will be analyzed with regard to sequence
in order to search for domains of pp60v-src which are critical for
transformation. This collection of mutants is unique in that each member
is (a) naturally occurring (implying no investigator bias), and (b) a point
mutant (implying a relatively subtle lesion).
这些研究的长期目标是确定独特的生化步骤
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MICHAEL F VERDERAME其他文献
MICHAEL F VERDERAME的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MICHAEL F VERDERAME', 18)}}的其他基金
相似海外基金
Investigating ubiquitination-regulated cell cycle events underpinning malaria transmission
研究泛素化调节的细胞周期事件支撑疟疾传播
- 批准号:
MR/Y013174/1 - 财政年份:2024
- 资助金额:
$ 13.89万 - 项目类别:
Research Grant
Investigating cell cycle vulnerabilities in TP53 mutant cancers
研究 TP53 突变癌症的细胞周期脆弱性
- 批准号:
MR/Y01264X/1 - 财政年份:2024
- 资助金额:
$ 13.89万 - 项目类别:
Research Grant
Conference: FASEB Yeast Chromosome and Cell Cycle Conference 2024
会议:2024 年 FASEB 酵母染色体和细胞周期会议
- 批准号:
2403471 - 财政年份:2024
- 资助金额:
$ 13.89万 - 项目类别:
Standard Grant
MRC TS Award: Regulation of neutrophil functions by cell cycle proteins
MRC TS 奖:细胞周期蛋白调节中性粒细胞功能
- 批准号:
MR/X023087/1 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别:
Fellowship
Developmental regulation of the cell cycle machinery
细胞周期机制的发育调控
- 批准号:
10714634 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别:
Cell cycle timing and molecular mechanisms of structural variant formation following incomplete replication
不完全复制后结构变异形成的细胞周期时间和分子机制
- 批准号:
10656861 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别:
Cell cycle control of cell polarity and fate in epidermal morphogenesis
表皮形态发生中细胞极性和命运的细胞周期控制
- 批准号:
10608036 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别:
Cell cycle-dependent dynein adaptor switching
细胞周期依赖性动力蛋白适配器转换
- 批准号:
23KF0285 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Regulation of Cell Cycle progression by the nuclear envelope
核膜对细胞周期进程的调节
- 批准号:
10659597 - 财政年份:2023
- 资助金额:
$ 13.89万 - 项目类别: