课题基金 / 基金详情

MOLECULAR CYTOGENETICS OF PROSTATE CANCER

MOLECULAR CYTOGENETICS OF PROSTATE CANCER
前列腺癌的分子细胞遗传学
批准号:
2099461
负责人:
Amato J. Giaccia
金额:
$19.1万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-02-01 至 1996-01-31

项目摘要

项目成果

Amato J. Giaccia的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
The incidence of prostate cancer is rapidly overcoming all other cancers in men 50 years and older and is now the second leading cause of cancer deaths in men. Prostate cancer can be broadly classified into three forms: latent, aggressive and metastatic. Each of these forms can be pathologically "staged" with respect to their anatomical location in or beyond the prostate and their degree of differentiation (Gleason's grade). Since a tumor results from a series of chromosomal alterations which allow the cell to escape from the normal mechanisms which control its growth, then it would be a logical first step to identify the specific chromosomal changes which are associated with tumor development. However, a paucity of information exists on the genetic and molecular evolution of events which are responsible for prostate cancer. To date, most attempts at identifying karyotypic chromosomal rearrangements in prostate cancer biopsies have proven to be frustrating. In part this is due to the cellular growth characteristics of prostate cancers which can lie dormant for months before division. In addition to their slow growth rate, obtaining metaphases from prostate cancer biopsies also has the inherent problem of selection for rapidly growing cells, biasing the results to cells that may not be representative of the tumor. Therefore, to avoid the problems associated with conventional cytogenetic banding techniques, we intend to apply the techniques of fluorescent in situ hybridization combine with premature chromosome condensation to karyotype prostate cancer cells from biopsies. With this new approach, the need to grow cells for even short periods of time can be avoided, and the population of cells to be analyzed is only limited by the size of the biopsy. this will allow direct analysis of tumor cells in situ, a goal that is difficult to achieve by conventional cytogenetic analysis because of the requirement for cell cultures to obtain metaphase chromosomes for banding analysis. We will use chromosome specific DNA libraries as probes to detect gross structural aberrations for each human chromosome; chromosome specific repetitive probes such as alpha satellite DNA (centromere specific probes) to detect numerical chromosome changes; and cosmid or YAC (Yeast Artificial Chromosomes) probes specific for microchromosomal regions that have putatively been implicated in prostate cancer such as 7q24 and 10q24 as well as those we will find during the course of this study. The ultimate goal of this study is to identify chromosome alterations which are stage or differentiation specific for prostate cancer. This goal has not yet been achieved for prostate cancer, mainly due to the problems of obtaining sufficient material for cytogenetic analysis. In addition, knowledge of known cytogenetic changes in prostate cancers will be useful both for prognosis and detection of minimal residual disease.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Project 1: Inhibition of Complement C5aR1 Radioprotects Normal Tissue and Radiosensitizes Tumors
  • 批准号:
    10707880
  • 项目类别:
  • 资助金额:
    $22.78万
  • 财政年份:
    2022
  • 负责人:
    Amato J. Giaccia
  • 依托单位:
Project 1: Inhibition of Complement C5aR1 Radioprotects Normal Tissue and Radiosensitizes Tumors
  • 批准号:
    10334199
  • 项目类别:
  • 资助金额:
    $25.93万
  • 财政年份:
    2022
  • 负责人:
    Amato J. Giaccia
  • 依托单位:
Preclinical Testing of a Novel Therapy Targeting AXL in Advanced Kidney Cancer
  • 批准号:
    8949353
  • 项目类别:
  • 资助金额:
    $50.78万
  • 财政年份:
    2016
  • 负责人:
    Amato J. Giaccia
  • 依托单位:
The Impact of Mitochondrial Repression and Lipid Accumulation by HIF on Tumor Growth
  • 批准号:
    10212325
  • 项目类别:
  • 资助金额:
    $67.29万
  • 财政年份:
    2015
  • 负责人:
    Amato J. Giaccia
  • 依托单位:
海外基金