FIBER-INDUCED DNA DAMAGE & CARCINOGENICITY

纤维引起的 DNA 损伤

• 批准号: 2108198
• 负责人: BISHARAH LIBBUS
• 金额: $ 7.63万
• 依托单位: INTEGRATED LABORATORY SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2108198
• 关键词: DNA damage; asbestos; carcinogen testing; carcinogens; ceramics; gel electrophoresis; glass; method development; mutagen testing; mutagens; tissue /cell culture

The introduction and increasing use of several man-made and synthetic fibers have raised concern over their possible health effects in view of their similarity to asbestos, a known carcinogen. Animal studies have clearly indicated the carcinogenic effects of asbestos fibers, Furthermore, the effects of asbestos on cultured cells include cytotoxicity and the induction of chromosome aberrations and cell transformation. We have recently documented the induction of DNA strand damage by asbestos using nick translation. We propose to use the induction of DNA for evaluating fiber genotoxicity and carcinogenicity. Two techniques, nick translation and the single cell gel (SCG) assay, will be used to measure DNA damage induced by asbestos fibers in cultured rat mesothelial cells. The more sensitive technique will then be utilized to quantitate the damage induced by a number of asbestiform, man-made and synthetic fibers. The induced damage will be correlated with animal carcinogenicity data available for the same fibers. Our goal is to determine the applicability of DNA damage assays to anticipate carcinogenicity of natural and artificial fibers.

几种人造和合成药物的引进和日益增加的使用 纤维引起了人们对它们可能对健康造成的影响的关注， 它们与已知的致癌物质石棉相似。 动物研究 清楚地表明了石棉纤维的致癌作用， 此外，石棉对培养细胞的影响包括 细胞毒性和诱导染色体畸变和细胞 转型 我们最近记录了DNA链的诱导 使用缺口平移的石棉损伤。 我们建议使用 DNA诱导用于评估纤维遗传毒性和致癌性。 两种技术，缺口平移和单细胞凝胶（SCG）测定，将 石棉纤维对培养大鼠DNA损伤的检测 间皮细胞 然后将利用更敏感的技术来 量化了石棉、人造石棉和 合成纤维。 诱发的损伤与动物 相同纤维的致癌性数据。 我们的目标是 确定DNA损伤检测的适用性， 天然和人造纤维的致癌性。