NEUTRALIZING MONOCLONAL ANTIBODIES TO MAITOTOXIN

将选择中和舞毒鱼毒素的鼠单克隆抗体 使用利用maitotoxin的溶血作用的功能测定。 所得抗体应可用于若干目的。 他们将 提供一种测量鱼类粗提取物中毒素含量的方法 组织或甲藻培养物通过溶血中和测定。 更重要的是，中和性单克隆抗体可能提供一种手段， 被动保护或逆转舞毒鱼毒素中毒。 这些试剂可以 用于临床治疗应用。 抗体可以 也可用于其他目的，包括研究受体 蝎毒素和钙离子通道的药理学;鉴定和 分离产生甲藻毒素的甲藻;以及 用免疫亲和层析法测定美托鱼毒素。 抗独特型抗体 中和舞毒鱼毒素的那些也应该是各种 分析和生物医学应用。

Murine monoclonal antibodies which neutralize maitotoxin will be selected using a functional assay that exploits the hemolytic action of maitotoxin. The resulting antibodies should be useful for several purposes. They will provide a means to measure the toxin content in crude extracts of fish tissues or dinoflagellate cultures by hemolysis neutralization assay. More importantly, neutralizing monoclonal antibodies may provide a means to passively protect or reverse maitotoxin poisoning. Such reagents could be humanized for clinical therapeutic applications. The antibodies may also be useful for other purposes including, studying the receptor pharmacology of maitotoxin and calcium ion channels; identifying and isolating maitotoxin-producing dinoflagellates; and, isolating purified maitotoxin by immunoaffinity chromatography. Anti-idiotypic antibodies of those which neutralize maitotoxin should also be of interest for a variety of analytical and biomedical applications.