NEUTRALIZING MONOCLONAL ANTIBODIES TO MAITOTOXIN

中和麦芽毒素单克隆抗体

• 批准号: 2155783
• 负责人: Gary Steven Bignami
• 金额: $ 8.03万
• 依托单位: HAWAII BIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2155783
• 关键词: Dinoflagellates; animal extract; antibody specificity; antibody titering; antitoxins; bovine serum albumin; chemical conjugate; enzyme linked immunosorbent assay; fish; fish poison; haptens; hemocyanin; hemolysis; laboratory mouse; method development; microorganism toxin; monoclonal antibody; neutralizing antibody; okadaic acid

Murine monoclonal antibodies which neutralize maitotoxin will be selected using a functional assay that exploits the hemolytic action of maitotoxin. The resulting antibodies should be useful for several purposes. They will provide a means to measure the toxin content in crude extracts of fish tissues or dinoflagellate cultures by hemolysis neutralization assay. More importantly, neutralizing monoclonal antibodies may provide a means to passively protect or reverse maitotoxin poisoning. Such reagents could be humanized for clinical therapeutic applications. The antibodies may also be useful for other purposes including, studying the receptor pharmacology of maitotoxin and calcium ion channels; identifying and isolating maitotoxin-producing dinoflagellates; and, isolating purified maitotoxin by immunoaffinity chromatography. Anti-idiotypic antibodies of those which neutralize maitotoxin should also be of interest for a variety of analytical and biomedical applications.

选择能中和maitotoxin的小鼠单克隆抗体