NEUTRALIZING MONOCLONAL ANTIBODIES TO MAITOTOXIN

将选择中和蜂毒毒素的鼠源性单抗 使用一种利用毛霉毒素的溶血作用的功能性测试。 由此产生的抗体应该对几个目的有用。他们会 提供一种测量鱼粗提物中毒素含量的方法 组织或甲藻培养的溶血中和试验。 更重要的是，中和单抗可能提供了一种方法 被动保护或逆转毛霉毒素中毒。这样的试剂可能 为临床治疗应用而人性化。抗体可以 也可用于其他目的，包括研究受体 毛霉毒素和钙离子通道的药理学；鉴定和 分离产生毛霉毒素的甲藻；以及分离纯化 用免疫亲和层析法测定蜂毒素。猪瘟抗独特型抗体 那些中和毛霉毒素的品种也应该是有兴趣的。 分析和生物医学应用。

Murine monoclonal antibodies which neutralize maitotoxin will be selected using a functional assay that exploits the hemolytic action of maitotoxin. The resulting antibodies should be useful for several purposes. They will provide a means to measure the toxin content in crude extracts of fish tissues or dinoflagellate cultures by hemolysis neutralization assay. More importantly, neutralizing monoclonal antibodies may provide a means to passively protect or reverse maitotoxin poisoning. Such reagents could be humanized for clinical therapeutic applications. The antibodies may also be useful for other purposes including, studying the receptor pharmacology of maitotoxin and calcium ion channels; identifying and isolating maitotoxin-producing dinoflagellates; and, isolating purified maitotoxin by immunoaffinity chromatography. Anti-idiotypic antibodies of those which neutralize maitotoxin should also be of interest for a variety of analytical and biomedical applications.