ORGANIC PHASE IMMUNOASSAY FOR CHLORINATED DIBENZODIOXINS

氯化二苯并二恶英的有机相免疫测定法

• 批准号: 3496687
• 负责人: Gary Steven Bignami
• 金额: $ 5万
• 依托单位: HAWAII BIOTECH, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496687
• 关键词: antigen antibody reaction; dioxins; environmental contamination; environmental toxicology; ligands; method development; monoclonal antibody; nonwater solvent; radioimmunoassay; receptor binding; soil sampling; toluene; tritium

High affinity antigen-antibody binding in essentially anhydrous hydrophobic organic solvents has been demonstrated in our laboratory. Using an estradiol-antiestradiol system, a prototype organic phase radioimmunoassay (OPRIA) has been developed which detects 200 pg/ml or estradiol in a hexane medium containing less than 0.1% (w/w) water. The proposed research will result in the development of an OPRIA for polychlorinated dibenzo-p-dioxins (PCDDs). The assay will employ monoclonal antibodies developed at Lawrence Livermore National Laboratory an the cross-reactive antigen, [3H]1-N- acetamido-3,7,8-trichlorodibenzo-p-dioxin ([3H]ATriCDD). Atricdd is not a regulated hazardous waste, due to its low toxicity. The hypothesis that an OPRIA for PCDDs will allow direct analysis of these pollutants in organic solvent sample extracts will be tested. If successful, this technology will significantly reduce the cost and time required to screen water, soil, and other environmental samples potentially contaminated with PCDDS.

在基本上无水的疏水介质中的高亲和力抗原-抗体结合 有机溶剂在我们的实验室已经得到证实。 使用 雌二醇-抗雌二醇系统，原型有机相放射免疫分析 已经开发了在己烷中检测200 pg/ml雌二醇的OPRIA 含水量低于0.1%（w/w）的培养基。 拟议的研究将 导致制定了多氯二苯并对二恶英的OPRIA （PCDDs）。 该检测将采用劳伦斯开发的单克隆抗体 利弗莫尔国家实验室和交叉反应抗原，[3 H]1-N- 乙酰氨基-3，7，8-三氯二苯并-p-二恶英（[3 H]ATriCDD）。 Atricdd不是一个 危险废物，因为它的毒性低。 假设一个 多氯二苯并对二恶英的开放式放射免疫分析将允许直接分析有机污染物中的这些污染物。 将测试溶剂样品浸提液。 如果成功，这项技术 将显著减少筛选水，土壤， 以及其他可能受到多氯二苯并对二恶英污染的环境样本。