PROSTAGLANDIN SYNTHASE-2--PURIFICATION AND IMMUNOASSAY

该项目的长期目标是开发大型 人前列腺素H合酶-2的规模生产和定量 （PGHS-2），一种最近发现的差异调节的酶， 具有与pGHS-1显著不同的性质。 获得的数据 在实验系统中的数据表明，PGHS-2亚型发挥作用， 在炎症、生殖中起重要作用， 致癌作用 用于大规模生产人PGHS-2 cDNA作为免疫调节剂来源的方法 人PGHS-2。 可获得大量纯化的、代谢活性的人 PGHS-2将加速非甾体类抗肿瘤药物的开发。 炎性药物（NSAID），对PGHS-2的选择性，以及 编码这些酶的mRNA分子，将允许监测 这些酶在血小板、白细胞和其他临床 标本 针对这些酶的特异性酶免疫测定将加速 PGHS异构体在不同组织中的差异表达研究 疾病状态，并监测个人服用低剂量阿司匹林的基础上， 其已报道的抗血栓形成和/或抗癌活性。

The long term objective of this project is to develop methods for large scale production and quantification of human prostaglandin H synthase-2 (PGHS-2), a recently discovered enzyme that is differentially regulated and has significantly different properties than the pGHS-1. Data obtained to date in experimental systems suggest that the PGHS-2 isoform plays a significant role in inflammation, reproduction, and possibly carcinogenesis. Methods for large scale production of human PGHS-2 cDNA as the source of human PGHS-2. Availability of large quantities of purified, metabolically active human PGHS-2 will permit accelerated development of non-steroidal anti- inflammatory drugs (NSAIDs) that are selective for PGHS-2, as well as the mRNA molecules that encode these enzymes, would permit the monitoring of the levels of these enzymes in platelets, leukocytes, and other clinical specimens. Specific enzyme immunoassays for these enzymes will accelerate research on the differential expression of PGHS isoforms in different disease states, and to monitor individuals taking low dose aspirin based on its reported antithrombotic and/or anticancer activities.