Fetal Alcohol Syndrome Biomarkers by Antibody Microarr

描述(由申请人提供)：胎儿酒精综合症(FAS)被定义为酗酒妇女所生孩子的一种发育迟缓、特征性面部畸形和智力低下的模式。及早开始对Fas儿童进行补救治疗是至关重要的。因此，人们正在寻找与注定会患上Fas的婴儿有关的生物标记物。Fas生物标志物的发现也有助于确定酒精致畸的潜在生理机制。我们的长期目标是为大鼠、小鼠和人类制作抗体微阵列，并筛选各种生物液和组织，以识别Fas和其他胎儿酒精效应的化学、基因和途径生物标记物。我们将为选定的(A)主要与氧化应激有关的蛋白质和化学物质，(B)通过蛋白质磷酸化参与细胞信号传递的磷酸化/非磷酸化蛋白质对，以及(C)具有生物活性的脂肪酸及其乙酯，生产假说驱动的靶向抗体阵列。我们还将生产蛋白质芯片来筛选自身抗体和蛋白质之间的相互作用。第一阶段，我们将生产大鼠氧化应激芯片和磷酸化/非磷酸化蛋白质对芯片，并通过筛选从胎肝组织和大鼠胎盘获得的蛋白质来寻找Fas的生物标志物。此外，我们还将生产生物活性脂肪酸的抗体。

DESCRIPTION (provided by applicant): Fetal Alcohol Syndrome (FAS) is defined as a pattern of growth retardation, characteristic facial anomalies and mental retardation in children born to alcoholic women. It is essential to begin remedial treatment of FAS children as early as possible. Therefore biomarkers linked to infants destined to develop FAS are being sought. The discovery of biomarkers for FAS could also help to identify potential physiological mechanisms that mediate alcohol teratogenesis. Our Iong-term goal is to produce antibody microarrays for rat, mouse and human and screen various biological fluids and tissues to identify chemical, gene and pathway biomarkers for FAS and other fetal alcohol effects. We will produce hypothesis-driven targeted antibody arrays for selected (a) proteins and chemicals primarily involved with oxidative stress, (b) phosphorylated/non-phosphorylated protein pairs involved in cellular signaling through protein phosphorylation and (c) biologically active fatty acids and their ethyl esters. We will also produce protein chips to screen autoantibodies and protein-protein interactions. Durin.q Phase I, we will produce rat oxidative stress chips and phosphorylated/non-phosphorylated protein pair chips and biomarkers of FAS will be searched by screening proteins obtained from fetal liver tissues and rat placentas. In addition, we will produce antibodies for biologically active fatty acids.