CONTROL OF OOCYTE MATURATION, IVF AND EMBRYO DEVELOPMENT

控制卵母细胞成熟、IVF 和胚胎发育

• 批准号: 2198370
• 负责人: ROBERT H FOOTE
• 金额: $ 21.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2198370
• 关键词: cell differentiation; cow; early embryonic stage; egg /ovum; embryo /fetus culture; embryo /fetus transplantation; extracellular matrix; fertilization; graafian follicles; growth factor; in vitro fertilization; laboratory mouse; laboratory rabbit; mammalian embryology; meiosis; sperm

This application addresses key aspects of the research team's long range goals to identify and characterize factors critical to in vitro oocyte maturation (IVM) and fertilization (IVF), and the early development of mammalian embryos. Cattle and rabbits have been selected as experimental models because they are excellent species for comparative studies and are consistent with superb university facilities available for this project. Oocytes from ovaries of calves and slaughterhouse cattle, and embryos subsequently derived from these studies in vitro as well as in vivo following superovulation of cattle and rabbits, will provide a rich source of experimental material to complete the experiments described. Under the first three specific aims, regulation of events necessary for acquisition of full meiotic competence and maturation of bovine oocytes will be determined for oocytes from (1) pre-antral follicles in comparison with those from (2) small and (3) larger antral follicles. Expertise in follicular growth and differentiation, IVM and IVF will be combined to establish molecular components and other culture conditions required for optimal recovery of follicular oocytes which will support a high rate of IVM, IVF, cleavage and subsequent normal embryo development following transfer. Logically following are specific aims (4) and (5) which focus on molecular mechanisms that regulate the development of bovine and rabbit embryos cultured in defined media recently developed by this group. These novel studies provide a unique opportunity to establish conditions required for in vitro development of completely competent embryos. The expected outcome is a substantial increase in knowledge of essential regulatory elements in vitro that control growth and maturation of oocytes, fertilization and the production of viable mammalian embryos. This information can be applied to developing IVM, IVF and embryo culture procedures which can be used to address fundamental questions in development and for the production of live young from domestic and wildlife species of special concern.

该应用程序解决了研究团队长期研究的关键问题， 目的是确定和表征体外卵母细胞的关键因素 成熟（IVM）和受精（IVF），以及 哺乳动物胚胎 牛和兔子被选作实验动物 因为它们是比较研究的优秀物种， 与该项目可用的一流大学设施相一致。 来自小牛和屠宰场牛卵巢的卵母细胞和胚胎 随后从这些体外和体内研究中获得 在牛和兔子超数排卵后， 实验材料来完成所描述的实验。 在前三个具体目标下， 牛卵母细胞完全减数分裂能力的获得和成熟 将测定来自（1）腔前卵泡的卵母细胞， 与来自（2）小窦卵泡和（3）大窦卵泡的那些相比。 专门知识 卵泡生长和分化，IVM和IVF将结合起来， 建立分子组分和其它培养条件 卵泡卵母细胞的最佳回收率，这将支持高的 IVM，IVF，卵裂和随后的正常胚胎发育 转移 从逻辑上讲，以下是具体目标（4）和（5），重点是 调控牛和兔发育的分子机制 在该小组最近开发的成分确定的培养基中培养的胚胎。 这些 新的研究提供了一个独特的机会，建立所需的条件， 用于完全有能力的胚胎的体外发育。 预期的结果是， 控制卵母细胞生长和成熟的体外调节元件， 受精和生产可行的哺乳动物胚胎。 这 这些信息可以应用于开发IVM，IVF和胚胎培养 可用于解决以下基本问题的程序： 发展和生产活的年轻从国内和野生动物 特别关注的物种。