CONTROL OF OOCYTE MATURATION, IVF AND EMBRYO DEVELOPMENT

控制卵母细胞成熟、IVF 和胚胎发育

• 批准号: 3552519
• 负责人: ROBERT H FOOTE
• 金额: $ 20.68万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3552519
• 关键词: cell differentiation; cow; early embryonic stage; egg /ovum; embryo /fetus culture; embryo /fetus transplantation; extracellular matrix; fertilization; graafian follicles; growth factor; in vitro fertilization; laboratory mouse; laboratory rabbit; mammalian embryology; meiosis; sperm

This application addresses key aspects of the research team's long range goals to identify and characterize factors critical to in vitro oocyte maturation (IVM) and fertilization (IVF), and the early development of mammalian embryos. Cattle and rabbits have been selected as experimental models because they are excellent species for comparative studies and are consistent with superb university facilities available for this project. Oocytes from ovaries of calves and slaughterhouse cattle, and embryos subsequently derived from these studies in vitro as well as in vivo following superovulation of cattle and rabbits, will provide a rich source of experimental material to complete the experiments described. Under the first three specific aims, regulation of events necessary for acquisition of full meiotic competence and maturation of bovine oocytes will be determined for oocytes from (1) pre-antral follicles in comparison with those from (2) small and (3) larger antral follicles. Expertise in follicular growth and differentiation, IVM and IVF will be combined to establish molecular components and other culture conditions required for optimal recovery of follicular oocytes which will support a high rate of IVM, IVF, cleavage and subsequent normal embryo development following transfer. Logically following are specific aims (4) and (5) which focus on molecular mechanisms that regulate the development of bovine and rabbit embryos cultured in defined media recently developed by this group. These novel studies provide a unique opportunity to establish conditions required for in vitro development of completely competent embryos. The expected outcome is a substantial increase in knowledge of essential regulatory elements in vitro that control growth and maturation of oocytes, fertilization and the production of viable mammalian embryos. This information can be applied to developing IVM, IVF and embryo culture procedures which can be used to address fundamental questions in development and for the production of live young from domestic and wildlife species of special concern.

这个应用程序解决了研究团队长期研究的关键方面 目的鉴定和表征影响体外卵母细胞发育的关键因素 成熟(IVM)和受精(IVF)与胚胎早期发育 哺乳动物的胚胎。牛和兔子被选为实验动物 模型，因为它们是比较研究的优秀物种，而且 与这个项目可用的一流大学设施相一致。 来自小牛和屠宰场牛卵巢的卵母细胞和胚胎 随后从这些体外和体内研究中衍生出来 继牛和兔的超数排卵后，将提供丰富的来源 完成所述实验的实验材料。 根据前三个具体目标，对以下活动的监管是必要的 牛卵母细胞完全减数分裂能力的获得和成熟 将被确定为来自(1)腔前卵泡的卵母细胞 来自(2)小卵泡和(3)大有腔卵泡的卵泡。以下领域的专业知识 卵泡的生长和分化、体外成熟和体外受精将结合起来 建立所需的分子组成和其他培养条件 卵泡卵母细胞的最佳回收，将支持高比率 体外受精、体外受精、卵裂和随后的正常胚胎发育 调职。逻辑上紧随其后的是重点关注的具体目标(4)和(5 牛和兔发育调控的分子机制 该小组最近开发了在特定培养液中培养的胚胎。这些 新颖的研究提供了一个独特的机会来建立所需的条件 用于体外发育完全有能力的胚胎。 预期的结果是实质性地增加对基本知识的了解 控制卵母细胞生长和成熟的体外调节元件， 受精和生产可存活的哺乳动物胚胎。这 信息可以应用于体外受精、体外受精和胚胎培养 可用于解决以下基本问题的过程 开发和生产家养和野生动物的活幼鱼 特别关注的物种。