DYNAMICS OF SPERM MEMBRANES, FERTILIZATION AND EMBRYOS

精子膜、受精和胚胎的动力学

• 批准号: 3552516
• 负责人: ROBERT H FOOTE
• 金额: $ 11.99万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3552516
• 关键词: acrosome; cell membrane; cow; early embryonic stage; egg /ovum; embryo /fetus culture; embryo /fetus transplantation; fertilization; image enhancement; in vitro fertilization; laboratory rabbit; mammalian embryology; membrane lipids; mixed tissue /cell culture; sperm; sperm capacitation; video recording system

The long term objective is to better understand the dynamic process of mammalian fertilization. The development of many tools in molecular biology and the emerging technologies for in vitro maintenance of sperm cells, oocytes and finally fertilization in vitro enhances the opportunity to isolate and identify critical morphological, biochemical and physiological changes vital to the formation and development of a normal conceptus. Specifically, the objectives of this research are: (1) to characterize changes in lipid composition of sperm call membranes associated with capacitation and the acrosome reaction; (2) to improve existing systems of in vitro fertilization; (3) to record on video tape and analyze through computer-enhanced image analysis the dynamic events surrounding in vitro fertilization; and finally (4) to extend this knowledge and technology to the study of early post-fertilization development. Much of the previous research in this area has involved a series of isolated observations. By combining the techniques of biochemical and ultrastructural analysis at various stages in well-controlled systems, and continuously recording changes associated with fertilization this research should be helpful in interpreting and integrating the dynamic events associated with fertilization. The development of these techniques will (1) facilitate the opportunity to detect when and how various environmental conditions impinge on early development and (2) directly improve the process of in vitro fertilization in humans and in animals.

长期目标是更好地了解 哺乳动物受精 分子生物学中许多工具的发展 生物学和体外精子维持的新兴技术 细胞、卵母细胞和最后的体外受精提高了机会 分离和鉴定关键的形态学、生化和 生理变化对正常组织的形成和发展至关重要 孕体 具体而言，本研究的目标是：（1）表征 精子膜脂质成分的变化与 获能和顶体反应;（2）改进现有的 体外受精;（3）用录像带记录并通过 计算机增强图像分析体外动态事件 施肥;最后（4）将这些知识和技术推广到 受精后早期发育的研究。 以前在这一领域的许多研究都涉及一系列 孤立的观察 通过结合生物化学技术和 在良好控制的系统中的各个阶段进行超微结构分析，以及 这项研究连续记录了与施肥有关的变化， 应该有助于解释和整合动态事件 与受精有关。 这些技术的发展将 (1)促进机会，以检测何时以及如何各种环境 条件冲击早期发展和（2）直接改善 人类和动物的体外受精过程。