5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids

在亚细胞和分子水平上快速和准确地量化活的生物流体性质构成了生物流体科学的基石。大多数生物流体设备依赖于在体外流式细胞仪(FC)中将其从体内移除后对其进行量化。FC面临许多警告，如生物降解和小体积等。在这个项目中，我们将设计第一台活体内5D成像流式细胞仪(5D IFC)，能够在不清除体内液体的情况下连续评估活着的小鼠的潜在整个血液容量。该项目代表着超越任何现有流式细胞仪的重大进步，并克服了最先进的激光扫描成像设备的工程限制。

Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging flow cytometer (5D IFC) capable of continuous assessment of potentially entire blood volume in a living mice without removing fluid out of the body. The project represents a major advancement beyond any existing flow cytometer and overcome the engineering limits of state-of-art laser scanning imaging devices.