DELETION MAPPING OF THE ZEBRAFISH GENOME
斑马鱼基因组的缺失图谱
基本信息
- 批准号:2283611
- 负责人:
- 金额:$ 5.23万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-09-15 至 1995-09-14
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Adapted from the applicant's abstract): The application
proposes to identify cellular and molecular components that contribute
to the differentiation and spatial organization of tissues in the early
vertebrate embryo. One avenue that has proven particularly informative
for elucidating how individual cell fates and overall body plan are
determined in invertebrates has been the analysis of developmental
mutations. Recent studies with the zebrafish (Brachydanio rerio) have
proven that this vertebrate has many of the attributes required for
developmental genetic studies. The longterm goal of the study is to
perform a detailed genetic and molecular characterization of genes that
contribute to early development and that lie in either of two regions
of the genome defined by the albino and yellow loci. The goal of this
application is to establish a series of overlapping deletion mutations
near the albino or yellow loci. Heritable deficiency mutations will be
induced following gamma irradiation of developing zebrafish embryos.
A total of 500 mutagenized fish will be mated with individuals harboring
recessive, viable, pigmentation mutations at albino or yellow to
identify the transmission of newly induced, non-complementing mutations.
Approximately 1 percent of the mutagenized fish are expected to harbor
a new mutation at each pre-specified locus. Each of the induced
mutations will be characterized genetically. The functions perturbed by
the mutations will be analyzed by examination of the phenotypes of
homozygous mutant embryos. Pairwise complementation tests between each
of the induced mutations will: 1) identify deletion mutations that
affect several gene functions; 2) help determine the minimal number of
distinct gene functions affected by each series of recessive lethal
mutations; and 3) permit construction of deficiency maps for the albino
and yellow regions of the genome. To demonstrate directly that some of
the rat-induced mutations are deletions, homozygous mutants will be
analyzed for loss of any of 50 specific DNA sequence loci. The proposed
project represents an efficient approach to the recovery of
developmental mutants in the zebrafish.The deletion mutations will
represent a first step toward complete genetic and molecular
characterization of the genes near albino and yellow that regulate early
development. Analysis of the developmental defects associated with these
mutations will reveal cellular and molecular functions that are essential
to early vertebrate embryogenesis.
描述(改编自申请人摘要):申请
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A mutation in zebrafish affecting a localized cellular function required for normal ear development.
斑马鱼的突变影响正常耳朵发育所需的局部细胞功能。
- DOI:10.1006/dbio.1996.0272
- 发表时间:1996
- 期刊:
- 影响因子:0
- 作者:Riley,BB;Grunwald,DJ
- 通讯作者:Grunwald,DJ
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DAVID J. GRUNWALD其他文献
DAVID J. GRUNWALD的其他文献
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{{ truncateString('DAVID J. GRUNWALD', 18)}}的其他基金
Establishing a new genetic mouse model of osteoarthritis
建立新型骨关节炎基因小鼠模型
- 批准号:
10260515 - 财政年份:2020
- 资助金额:
$ 5.23万 - 项目类别:
Establishing a new genetic mouse model of osteoarthritis
建立新型骨关节炎基因小鼠模型
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Gene targeting in zebrafish: building models to assay disease genes
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8684468 - 财政年份:2014
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Gene Targeting by Homologous Recombination in the Zebrafish
斑马鱼中同源重组的基因打靶
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8364774 - 财政年份:2012
- 资助金额:
$ 5.23万 - 项目类别:
Gene Targeting by Homologous Recombination in the Zebrafish
斑马鱼中同源重组的基因打靶
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8534228 - 财政年份:2012
- 资助金额:
$ 5.23万 - 项目类别:
Role of Intracellular Calcium Release in Hedgehog Growth Factor Signaling
细胞内钙释放在刺猬生长因子信号传导中的作用
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Role of Intracellular Calcium Release in Hedgehog Growth Factor Signaling
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- 资助金额:
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